An analytical method for dynamic tracer monitoring of nicotine and its metabolites in animals

Abstract

An analysis method for dynamic tracing and monitoring of nicotine and its metabolites in animals, characterized in that nicotine ([2H4]‑(±)‑Nicotine) labeled with a pyridine ring 2H is mixed in equal proportions with unlabeled nicotine After exposure to animals, use the microdialysis system to collect blood and brain dialysis fluid samples respectively in microcentrifuge tubes, add an internal standard for analysis, and centrifuge at low speed for a short time, and then directly load the samples into ultra-high performance liquid chromatography-Orbitrap high-resolution mass spectrometry analysis and detection microdialysis The nicotine metabolites and their pyridine ring 2H markers in the sample were identified according to the test results and the corresponding time-concentration curves were drawn for dynamic monitoring and analysis. The present invention has the advantages of accurately tracing and identifying various nicotine metabolites in animals, greatly simplifying the sample pretreatment steps, and realizing accurate analysis and synchronous comparison of changes in nicotine metabolites in different systems in animals. Created a new method for the study of nicotine metabolism in animals.

Description

technical field [0001] The invention relates to a method for analyzing nicotine metabolism in animals. In this method, stable isotopes are used to trace the metabolic process of nicotine, rat brain tissue and blood samples are collected synchronously through microdialysis technology, and nicotine metabolites in the samples are traced and detected by ultra-high performance liquid chromatography-Orbitrap high-resolution mass spectrometry Analysis, so as to comprehensively reveal the metabolic characteristics of nicotine in animals. Background technique [0002] Nicotine (Nic) is an important characteristic component of tobacco, and the occurrence of nicotine dependence is closely related to the individual's ability to metabolize nicotine. Although the nicotine ingested in the body will quickly pass through the blood-brain barrier and enter the brain tissue, most of the body's metabolism of nicotine is completed in the liver. The transformation and metabolism process of nicot...

AI Technical Summary

Problems solved by technology

This method uses a pyridine ring 2 H-labeled nicotine ([ 2 h 4 ]-(±)-Nicotine) to trace the metabolic transformation process of nicotine, combined with high-resolution mass spectrometry, not only has high sensitivity, can obtain accurate molecular mass information of metabolites, but also can use the pyridine ring 2 The pairing of H-labeled isotope peaks and unlabeled nicotine metabolite peaks further confirms the metabolite information of nicotine in vivo, especially in brain tissue, and overcomes the low sensitivity and easy radioactive signal detection of radioisotope labeling. In addition, this method also uses microdialysis sampling technology to realize the direct injection analysis of samples and the simultaneous and dynamic analysis of the metabolism of nicotine in the blood and brain systems of living rats.

Images