Preparation method of low temperature resistance polysaccharide microbial flocculant and application thereof

A microbial flocculant and low-temperature-resistant technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of saving production costs and good flocculation

Inactive Publication Date: 2016-08-31
克雷伯氏环保科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome the problem of preparation cost of the existing microbial flocculant, the present invention provides a preparation method of a low temperature resistan

Method used

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  • Preparation method of low temperature resistance polysaccharide microbial flocculant and application thereof

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Experimental program
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Effect test

Embodiment 1

[0022] The preparation method of this microbial flocculant, its step comprises:

[0023] (1) Take the Klebsiella oxytoca GS-4-08 strain cultured in the laboratory and grow aerobically at 35°C for 12 hours;

[0024] The components of the strain growth medium are: glucose 5.92g / L, KH 2 PO 4 2g / L, Na 2 HPO 4 12H 2 O 10.3g / L, NaCl 0.05g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, urea 2.13g / L, adjust the pH value to be 7; cultivate a large amount of Klebsiella oxytoca GS-4-08 bacterial classification that obtains, and the preservation number of this bacterial classification is CGMCCNo.5237;

[0025] (2) Add the culture solution when the aerobic growth reaches the end of exponential growth into the mixed waste water with a treatment volume of 1 liter according to the inoculum rate of 10%, and carry out aeration treatment;

[0026] Respectively adjust the ratio of sucrose factory wastewater and nitrile wastewater in the mixed wastewater to be: 1:1, 3:1, 5:1, 10:1, 20...

Embodiment 2

[0033] The application of the microbial flocculant in the flocculation treatment of soybean product wastewater comprises the following steps:

[0034] To the COD concentration is 12000mg / L bean product former waste water (50mL), add microbial flocculant 0.3mg among the embodiment 1, leave standstill 10min after stirring 30s rapidly with the stirring speed of 240rpm, measure the COD concentration of waste water supernatant, The treatment water temperatures are 4, 25, 35, 45, 55, 65°C respectively. The results of COD removal rate are shown in Table 2. As can be seen from Table 2, when the present invention is treated at room temperature, the COD removal efficiency of the bio-flocculant prepared by the present invention is higher to the bean product wastewater, and the COD removal efficiency of the bio-flocculant of the present invention is processed when the temperature is 4 It can also reach 81.16, which has high efficiency, which shows that the biological flocculant can treat...

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Abstract

The invention discloses a preparation method of a low temperature resistance polysaccharide microbial flocculant. The method comprises the following steps that strains Klebsiella oxytoca GS-4-08 are used for synchronous processing of saccharose wastewater and nitrile wastewater; the wastewater obtained after processing is utilized for alcohol precipitation, other steps are executed for collecting an extracellular polymeric substance secreted by bacterial strains, and the microbial flocculant is obtained. The invention further discloses application of the obtained microbial flocculant for processing wastewater, particularly bean products wastewater. The method has the advantages that according to the method of the produced microbial flocculant, the strains Klebsiella oxytoca GS-4-08 are used for synchronous processing of saccharose and nitrile mixed wastewater, the microbial flocculant produced by a carbon source and a nitrogen source in the wastewater is effectively utilized, no carbon source or nitrogen source needed for culturing the microbial flocculant needs to be added, and therefore a large number of production cost can be saved. The prepared microbial flocculant can perform flocculation on bean product wastewater, and the COD removal effect can reach 82.18%.

Description

technical field [0001] The invention relates to an energy-saving preparation of a low-temperature-resistant polysaccharide microbial flocculant and its application in flocculation treatment of various chemical wastewater, belonging to the technical field of industrial wastewater treatment. Background technique [0002] Microbial flocculant is a kind of viscous substance secreted by microorganisms during the growth process, mostly biological macromolecules of glycoprotein, proteoglycan or polysaccharide type. Its application in wastewater treatment can achieve the same treatment effect as chemical flocculants, but microbial flocculants have biodegradable characteristics, and their use in wastewater treatment will not cause secondary pollution to water bodies, which is environmentally friendly flocculant. However, at present, its preparation method mostly adopts the culture medium prepared in the laboratory for batch cultivation, and while the yield is high, it is also diffic...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12P19/04C12R1/22C02F103/26
CPCC02F3/34C02F2103/26C12P19/04
Inventor 虞磊
Owner 克雷伯氏环保科技(苏州)有限公司
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