Sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and preparation method of sunflower calathide extract
A technology of sunflower disc and extract, applied in the field of sunflower disc extract, can solve the problems of single application of sunflower disc and waste of resources, etc., and achieve the effect of remarkable clinical medical effect, easy operation and high safety
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Embodiment 1
[0031] Preparation steps of sunflower disk extract:
[0032] 1) Take 1 kg of sunflower disk and grind it, add 4 mass times of 50% ethanol to heat and reflux for extraction once, each time for 1 hour, combine the extracts, filter, and concentrate the filtrate under reduced pressure to 0.5 times the mass of the medicinal materials to obtain the alcohol extract;
[0033] 2), the alcohol extract obtained in step 1) is filtered, and the filtrate is taken;
[0034] 3) After the filtrate obtained in step 2) is fully adsorbed by 732 type strong acidic cation exchange resin, first wash away the water-soluble impurities with distilled water at a flow rate of 4BV / h, and then use 5 mass multiples of 50%% ethanol at 1BV / h The flow rate of h is eluted, the eluate is collected, concentrated under reduced pressure, and dried to obtain the total flavonoids of sunflower disc; its content is 61.4% in terms of rutin;
[0035] 4) The 732 type strong acidic cation exchange resin that has been elut...
Embodiment 2
[0040] Preparation steps of sunflower disk extract:
[0041] 1) Take 1 kg of sunflower discs and crush them, add 6 mass multiples of 75% ethanol to heat and reflux for extraction twice, each time for 2 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to 1 times the mass of the medicinal materials to obtain the alcohol extract;
[0042] 2), the alcohol extract obtained in step 1) is filtered, and the filtrate is taken;
[0043] 3) After the filtrate obtained in step 2) is fully adsorbed by HZ002 strong acidic cation exchange resin, the water-soluble impurities are washed away with distilled water at a flow rate of 5BV / h, and then 75% ethanol with 8 mass multiples is used at 2BV / h The flow rate is eluted, the eluate is collected, concentrated under reduced pressure, and dried to obtain the total flavonoids of sunflower disc; its content is 66.4% in terms of rutin;
[0044] 4) Elute the HZ002 type strongly acidic cation exchange resin that h...
Embodiment 3
[0049] Preparation steps of sunflower disk extract:
[0050] 1) Crush 1 kg of sunflower discs and add 10 mass multiples of 75% ethanol to heat and reflux for extraction 3 times, each time for 3 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to twice the mass of the medicinal material to obtain an alcohol extract;
[0051] 2), the alcohol extract obtained in step 1) is filtered, and the filtrate is taken;
[0052] 3) After the filtrate obtained in step 2) is fully adsorbed by HZ002 strong acidic cation exchange resin, first wash away water-soluble impurities with distilled water at a flow rate of 6BV / h, and then use 10 mass multiples of 95% ethanol at a concentration of 3BV / h flow rate for elution, collect the eluate, concentrate under reduced pressure, and dry to obtain the total flavonoids of sunflower disc; its content is 70.5% in terms of rutin;
[0053] 4) Elute the HZ002 type strongly acidic cation exchange resin that has been e...
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