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Indian kalimeris herb active component and compounds as well as preparation and application

A technology for active components and flavonoid glycoside compounds, applied in the field of medicine, can solve problems such as serious drug resistance, environmental pollution, etc., and achieve the effects of simple preparation method and high product purity

Active Publication Date: 2016-09-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long-term and extensive application of chemical pesticides has also produced serious drug resistance and environmental pollution

Method used

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  • Indian kalimeris herb active component and compounds as well as preparation and application
  • Indian kalimeris herb active component and compounds as well as preparation and application
  • Indian kalimeris herb active component and compounds as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The preparation method of Malan water-soluble active components and two flavonoid glycoside compounds kaempferol-3-O-rutinoside and isorhamnetin-3-O-rutinoside

[0024] (1) Crushing and extraction:

[0025] Take 1 kg of dried Malan and grind it, extract it twice with 10L methanol (industrial grade) at room temperature, and concentrate it by suction filtration for 1 day each time to obtain a crude sample of the methanol extract, extract and separate it with ethyl acetate and water, and let it stand , after concentrating the aqueous layer under reduced pressure and spinning to dryness, 28 g of a crude sample of the aqueous layer of B was obtained.

[0026] (2) Separation and purification:

[0027] The crude sample of the aqueous layer is first separated by an ODS open column (the solvent system is methanol: water = 3:7, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0 by volume) The active sample is separated with a silica gel open column (200-300 mesh, the following solvent sys...

Embodiment 1

[0029] Qualitative identification of the physical and chemical characteristics and chemical structure of the compound kaempferol-3-O-rutinoside and isorhamnetin-3-O-rutinoside obtained in Example 1:

[0030] The structures of compounds kaempferol-3-O-rutinoside and isorhamnetin-3-O-rutinoside have been 1 H NMR, MS and literature comparison confirmed

[0031] Physicochemical properties of compound kaempferol-3-O-rutinoside: yellow solid, Molecular formula is C 27 h 30 o 15 ; ESI-TOF-MS: m / z 595.1663 (M+H) + , hydrogen spectrum data: 1 H NMR (500MHz, CD 3 OD): δ=1.12(3H,d,J=6.0Hz,H-6"'),3.25-2.81(10H,m,H-2"-6" / 2"'-5"'),4.51( 1H, s, H-1"'), 5.12 (1H, d, J=7.5Hz, H-1"), 6.89 (2H, d, J=8.5Hz, H-3' / 5'), 8.07( 2H,d,J=8.5Hz,H-2' / 6'), 6.41(1H,s,H-8), 6.21(1H,s,H-6).

[0032] Physicochemical properties of compound isorhamnetin-3-O-rutinoside: yellow solid, Molecular formula is C 28 h 32 o 16 ,ESI-TOF-MS:m / z 625.1765(M+H) + , hydrogen spectral data: 1 H NMR (500MHz, CD ...

Embodiment 3

[0034] The water-soluble active components and compounds of Malan can significantly inhibit the production of Phytophthora sporangia in the screening model of Phytophthora capsici.

[0035] At present, the research on active substances against Phytophthora mainly focuses on inhibiting the growth of mycelia and germination of zoospores. There are very few studies on active substances that can inhibit the production of sporangia in the asexual reproduction stage of Phytophthora. Metalaxyl is currently the most widely used pesticide in the prevention and treatment of plant diseases. It can strongly inhibit the growth of Phytophthora mycelium and the production of asexual sporangia. The present invention finds that the water-soluble active component of Malan and its two flavonoid glycoside compounds, kaempferol-3-O-rutinoside and isorhamnetin-3-O-rutinoside, can significantly inhibit the production of Phytophthora sporangia in the Phytophthora capsici model.

[0036] 1. Experiment...

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Abstract

The invention provides an Indian kalimeris herb water-soluble active component and two flavonoid glycoside compounds. After being crushed, traditional Chinese medicine Indian kalimeris herb is extracted with methanol and then is filtered and concentrated; after distribution is carried out through water and an ethyl acetate solvent, a water layer is concentrated; ODS opening column separation is carried out and silica gel opening column separation is carried out to obtain the Indian kalimeris herb active component; the Indian kalimeris herb active component is purified to obtain the two flavonoid glycoside compounds. By inhibiting a biological active system generated by phytophthora sporangium, the Indian kalimeris herb active component and the two flavonoid glycoside compounds can be used for remarkably inhibiting the generation of the phytophthora sporangium, have an anti-phytophthora effect and can be used for preparing anti-phytophthora pesticides. A preparation method is simple and the purity of an extracted product is high; the Indian kalimeris herb is vegetable and traditional Chinese medicine and is low in price and easy to obtain; the medicine usage of the Indian kalimeris herb is expanded and evidences are provided for developing innovative pesticides by utilizing traditional Chinese medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a water-soluble active component and a compound of Malan, a preparation method and an application in preparation of a phytophthora anti-phytophthora pesticide. Background technique [0002] At present, there are more than 100 species of Phytophthora, which are increasing month by month. They can cause the most devastating plant diseases in the world and cause serious economic losses every year. In the middle of the 19th century, the late blight of potatoes caused by Phytophthora infestans led to the Great Irish Famine, which eventually caused the death of more than one million people and the human tragedy of more than one million people leaving their homes. Eucalyptus root rot caused by Phytophthora camphora has caused major changes in forest structure and plant populations in Southwest Australia, which has seriously threatened local biodiversity and natural ecosystems. The long-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/07A01N43/16A01P3/00
CPCA01N43/16C07H1/08C07H17/07
Inventor 戚建华张慧王源超
Owner ZHEJIANG UNIV
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