Embedded nano iron/two microbial agents and preparation method thereof
A microbial agent and nano-iron technology, which is applied in the field of triclosan wastewater treatment, can solve the problems of poor stability of nano-zero-valent iron, achieve the effects of low microbial toxicity, in-situ repair, and wide sources
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0036] (1) Preparation of triclosan-degrading bacterial liquid
[0037] Pick two rings of Pseudomonas putida. and Pseudomonas stutzeri. respectively, and transfer them to 30 mL of nutrient solution, and culture the bacteria at 35°C for 2 days to 10% by volume was inoculated into the proliferation medium, cultured at 35° C. for 2 days, centrifuged at 5000 rpm for 15 minutes, and the logarithmic growth phase cells of the above bacteria were obtained respectively.
[0038] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) and washed twice. According to volume percentage, 37% of Pseudomonas putida and 63% of Pseudomonas stutzeri were mixed to obtain Pseudomonas stutzeri thallus for triclosan degradation. The bacteria were suspended in saline and refrigerat...
Embodiment 2
[0053] (1) Preparation of triclosan-degrading bacterial liquid
[0054] Pick two rings of Pseudomonas putida. and Pseudomonas stutzeri. respectively, and transfer them to 30 mL of nutrient solution, and culture the bacteria at 35°C for 2 days to 10% by volume was inoculated into the container of the proliferation medium, cultured at 35° C. for 2 days, centrifuged at 5000 rpm for 15 minutes, and the logarithmic growth phase cells of the above bacteria were obtained respectively.
[0055] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) and washed twice. According to volume percentage, 37% of Pseudomonas putida and 63% of Pseudomonas stutzeri were mixed to obtain Pseudomonas stutzeri thallus for triclosan degradation. The bacteria were suspended in sali...
Embodiment 3
[0069] (1) Preparation of triclosan-degrading bacterial liquid
[0070] Pick two rings of Pseudomonas putida. and Pseudomonas stutzeri. respectively, and transfer them to 30 mL of nutrient solution, and culture the bacteria at 35°C for 2 days to 10% was inoculated into the container of the proliferation medium, cultured at 35° C. for 2 days, centrifuged at 5000 rpm for 15 minutes, and the logarithmic growth phase cells of the above bacteria were obtained respectively.
[0071] The logarithmic growth phase cells of the above-mentioned thalli are taken out, and with phosphate buffer (its main component sodium chloride 9.0g / L, potassium chloride 0.3g / L, dipotassium hydrogen phosphate 1.2g / L and potassium dihydrogen phosphate 0.3g / L, the rest is water) and washed twice. According to volume percentage, 37% of Pseudomonas putida and 63% of Pseudomonas stutzeri were mixed to obtain Pseudomonas stutzeri thallus for triclosan degradation. The bacteria were suspended in saline and ref...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More