Mushroom bacterial strain preparation method
A technology for strain preparation and shiitake mushrooms, applied in botany equipment and methods, mushroom cultivation, fertilizer mixture, etc., can solve the problems of long germination period of branch strains, time-consuming and labor-intensive seeding of sawdust, low incidence of strains, etc. Achieve the effects of inhibiting the growth of miscellaneous bacteria, prolonging the shelf life, and reducing the incidence rate
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[0020] Example 1
[0021] A method for preparing mushroom strains:
[0022] (1) Choose amorpha branches with a diameter of 0.5 cm and a length of 6 cm, boil them in boiling water for 20 minutes, and soak them in a pool containing chondroitin sulfate waste liquid for 36 hours. The ratio of chondroitin sulfate waste liquid to water in the pool is 1 :10. Chondroitin sulfate waste liquid is the waste liquid produced by the production of chondroitin by enzymatic hydrolysis. It contains 200mg / L of polysaccharides, 5000mg / L of amino acids, and 20mg / L of salt. Rinse, drain, and keep the water content of the branches at 50%;
[0023] (2) Put the branches, bran and water into a 17㎝*36㎝ corner bag according to the mass ratio of 4:1:0.2, stir evenly, the branches are full of bran, sterilize, and the conditions are 0.15Mpa, 120 Sterilize at ℃ for 2 hours;
[0024] (3) The inoculated grain strain is 7% of the branch weight, and after the mycelium is overgrown with the branches, continue to cultiva...
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[0027] Example 2
[0028] A method for preparing mushroom strains:
[0029] (1) Choose oak branches with a diameter of 0.5 cm and a length of 8 cm, put them in a pool of boiling water for 30 minutes, and soak them in a pool containing chondroitin sulfate waste liquid for 36 hours. The ratio is 1:50. The waste liquid of chondroitin sulfate is the waste liquid produced by the production of chondroitin by enzymatic hydrolysis. It contains 500mg / L of polysaccharides, 10000mg / L of amino acids, and 50mg / L of salt. Rinse, drain, and keep the branch water at 60%;
[0030] (2) Put the branches, bran and water into a 17㎝*36㎝ corner bag according to the mass ratio of 4:1:0.2, stir evenly, the branches are full of bran, sterilize, and the conditions are 0.15Mpa, 120 Sterilize at ℃ for 3 hours;
[0031] (3) The inoculated grain strain is 7% of the branch weight, and after the mycelium is overgrown with branches, continue to cultivate for 30 days;
[0032] (4) Oak sawdust, sweet-scented osmanthus, ...
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