Preparation method and quality control method of mulberry leaf extract

A quality control method, the technology of mulberry leaf extract, which is applied in the field of preparation of mulberry leaf extract, can solve the problems of increased probability of microbial failure, cumbersome operation, and decreased rutin content, so as to avoid the loss of rutin content and repeatability. Good, good extraction and retention effect

Inactive Publication Date: 2016-09-21
GUANGDONG YIFANG PHARMA
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the laboratory and large-scale production practice, it is found that the content of rutin in the extract obtained by this extraction method of mulberry leaves is low. The reasons may be as follows: (1) The mulberry leaves are washed with water before feeding and extraction. Some rutin may be lost; (2) During the high-temperature concentration process of the extract (concentration temperature is higher than 60°C), the rutin content will decrease, and as the concentration temperature rises and the concentration time prolongs, the rutin in the extract will The content showed a significant downward trend
If the mulberry leaves are fed directly without washing, the probability of unqualified microorganisms in the final product extract will increase. The reason is that the heating method of the large-scale extraction tank is interlayer steam heating. It is difficult to heat and boil, and the unwashed medicinal materials themselves have more microorganisms, resulting in the microorganisms in the extract not being killed, so that the microbial indicators of the extract are unqualified
[0003] In addition, Chinese Pharmacopoeia 2015 edition 1 mulberry leaf [Content Determination] was used to determine the content of rutin. The preparation method of the test solution was to use pure methanol to heat and reflux to extract twice. , the operation time is relatively long, and the reproducibility is poor
Chinese Pharmacopoeia 2015 Edition, Part One Sophora japonica [Content Determination] is used to determine the content of rutin. The preparation method of the test solution is to use pure methanol for ultrasonic extraction for 30 minutes. The operation is relatively simple, but the low water content in the extraction solvent may not be conducive to Rutin transfers, leading to low content results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and quality control method of mulberry leaf extract
  • Preparation method and quality control method of mulberry leaf extract
  • Preparation method and quality control method of mulberry leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of mulberry leaf extract

[0027] (1) Shred mulberry leaves, add water to decoct and extract twice, add 13 times the weight of water for the first time, boil, decoct and extract for 1.5 hours, add 8 times the weight of water for the second time, boil, decoct and extract for 1 hour , combined the two decocted filtrates, filtered through a 350-mesh sieve to obtain the initial filtrate;

[0028] (2) Take the primary filtrate and pass it through a microporous membrane with a pore size of 0.02 microns to obtain a fine filtrate; the control parameters of the microporous membrane are: P1≤1.8MPa, P2≤1.75MPa, filtrate temperature≤45°C, pressure difference control At 0.05~0.1MPa;

[0029] (3) Concentrate the refined filtrate through a reverse osmosis membrane with a pore size of 0.0005 microns to a solid content rate of 16% to obtain a concentrated initial solution; the control parameters of the reverse osmosis membrane are: P1≤1.8MPa, P2≤1.75MPa, f...

Embodiment 2

[0031] Embodiment 2: the preparation of mulberry leaf extract

[0032] (1) Shred mulberry leaves, add water to decoct and extract twice, add 12 times the weight of water for the first time, boil, decoct and extract for 1.0 hour, add 9 times the weight of water for the second time, boil, decoct and extract for 1.5 hours , combined the two decocted filtrates, filtered through a 350-mesh sieve to obtain the initial filtrate;

[0033] (2) Take the primary filtrate and pass it through a microporous membrane with a pore size of 0.01 microns to obtain a refined filtrate; the control parameters of the microporous membrane are: P1≤1.8MPa, P2≤1.75MPa, filtrate temperature≤45°C, pressure difference control At 0.05~0.1MPa;

[0034] (3) Concentrate the refined filtrate through a reverse osmosis membrane with a pore size of 0.001 microns to a solid content of 15%, and obtain the initial concentrated liquid; the control parameters of the reverse osmosis membrane are: P1≤1.8MPa, P2≤1.75MPa, ...

Embodiment 3

[0036] Embodiment 3: the preparation of mulberry leaf extract

[0037] (1) Shred mulberry leaves, add water to decoct and extract twice, add 10 times the weight of water for the first time, boil, decoct and extract for 1.2 hours, add 10 times the weight of water for the second time, boil, decoct and extract for 1.2 hours , combined the two decocted filtrates, filtered through a 350-mesh sieve to obtain the initial filtrate;

[0038] (2) Take the primary filtrate and pass it through a microporous membrane with a pore size of 0.02 microns to obtain a fine filtrate; the control parameters of the microporous membrane are: P1≤1.8MPa, P2≤1.75MPa, filtrate temperature≤45°C, pressure difference control At 0.05~0.1MPa;

[0039] (3) Concentrate the refined filtrate through a reverse osmosis membrane with a pore size of 0.0001 micron to a solid content rate of 18% to obtain the initial concentrated liquid; the control parameters of the reverse osmosis membrane are: P1≤1.8MPa, P2≤1.75MPa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method and a preparation control method of a mulberry leaf extract. The preparation method comprises the steps of cutting mulberry leaves into shreds, adding water, decocting and extracting for two times, combining two decoction-filtering liquors, filtering through a sieve, enabling a primary filtrate to pass through a microfiltration membrane with the bore diameter of 0.01 to 0.02 micron, enabling a refined filtrate to pass through a reverse osmosis membrane with the bore diameter of 0.0001 to 0.001 micron, concentrating until a solid content is 15 to 18 percent, performing vacuum concentration on a concentrated initial solution under 60 DEG C, to form an extractum with the relative density of 1.10 to 1.13, and spray-drying the extractum to obtain the mulberry leaf extract. A quality control method is characterized by adopting high efficiency liquid chromatography to measure the content of rutin in the mulberry leaf extract. The preparation method can avoid rutin content loss caused by washing a mulberry leaf medicinal material and performing high temperature concentration, the rutin in the mulberry leaves is well extracted and reserved, microorganisms such as bacteria can be well removed, and a microorganism indicator is enabled to meet national health food material regulations; the quality control method is accurate, reliable and good in repeatability, and can be taken as a method for measuring the content of the mulberry leaf extract rutin.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of mulberry leaf extract and a quality control method thereof. Background technique [0002] Mulberry leaf is a common Chinese herbal medicine, which contains rutin and other flavonoids. The Chinese Pharmacopoeia uses rutin as the content determination index to test and control the quality of mulberry leaf medicinal materials. The existing method for extracting mulberry leaf extract is to wash the mulberry leaves with water, then feed them and decoct them for extraction, and then concentrate the extract at high temperature. However, in the laboratory and large-scale production practice, it is found that the content of rutin in the extract obtained by this extraction method of mulberry leaves is low. The reasons may be as follows: (1) The mulberry leaves are washed with water before feeding and extraction. Some rutin may be l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605G01N30/02
CPCA61K36/605A61K2236/331A61K2236/39A61K2236/51A61K2236/53G01N30/02G01N2030/027
Inventor 罗艳萍程学仁张风张冬林
Owner GUANGDONG YIFANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap