Preparation method and quality control method of mulberry leaf extract

Abstract

The invention discloses a preparation method and a preparation control method of a mulberry leaf extract. The preparation method comprises the steps of cutting mulberry leaves into shreds, adding water, decocting and extracting for two times, combining two decoction-filtering liquors, filtering through a sieve, enabling a primary filtrate to pass through a microfiltration membrane with the bore diameter of 0.01 to 0.02 micron, enabling a refined filtrate to pass through a reverse osmosis membrane with the bore diameter of 0.0001 to 0.001 micron, concentrating until a solid content is 15 to 18 percent, performing vacuum concentration on a concentrated initial solution under 60 DEG C, to form an extractum with the relative density of 1.10 to 1.13, and spray-drying the extractum to obtain the mulberry leaf extract. A quality control method is characterized by adopting high efficiency liquid chromatography to measure the content of rutin in the mulberry leaf extract. The preparation method can avoid rutin content loss caused by washing a mulberry leaf medicinal material and performing high temperature concentration, the rutin in the mulberry leaves is well extracted and reserved, microorganisms such as bacteria can be well removed, and a microorganism indicator is enabled to meet national health food material regulations; the quality control method is accurate, reliable and good in repeatability, and can be taken as a method for measuring the content of the mulberry leaf extract rutin.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of mulberry leaf extract and a quality control method thereof. Background technique [0002] Mulberry leaf is a common Chinese herbal medicine, which contains rutin and other flavonoids. The Chinese Pharmacopoeia uses rutin as the content determination index to test and control the quality of mulberry leaf medicinal materials. The existing method for extracting mulberry leaf extract is to wash the mulberry leaves with water, then feed them and decoct them for extraction, and then concentrate the extract at high temperature. However, in the laboratory and large-scale production practice, it is found that the content of rutin in the extract obtained by this extraction method of mulberry leaves is low. The reasons may be as follows: (1) The mulberry leaves are washed with water before feeding and extraction. Some rutin may be l...

AI Technical Summary

Problems solved by technology

However, in the laboratory and large-scale production practice, it is found that the content of rutin in the extract obtained by this extraction method of mulberry leaves is low. The reasons may be as follows: (1) The mulberry leaves are washed with water before feeding and extraction. Some rutin may be lost; (2) During the high-temperature concentration process of the extract (concentration temperature is higher than 60°C), the rutin content will decrease, and as the concentration temperature rises and the concentration time prolongs, the rutin in the extract will The content showed a significant downward trend

If the mulberry leaves are fed directly without washing, the probability of unqualified microorganisms in the final product extract will increase. The reason is that the heating method of the large-scale extraction tank is interlayer steam heating. It is difficult to heat and boil, and the unwashed medicinal materials themselves have more microorganisms, resulting in the microorganisms in the extract not being killed, so that the microbial indicators of the extract are unqualified

[0003] In addition, Chinese Pharmacopoeia 2015 edition 1 mulberry leaf [Content Determination] was used to determine the content of rutin. The preparation method of the test solution was to use pure methanol to heat and reflux to extract twice. , the operation time is relatively long, and the reproducibility is poor

Chinese Pharmacopoeia 2015 Edition, Part One Sophora japonica [Content Determination] is used to determine the content of rutin. The preparation method of the test solution is to use pure methanol for ultrasonic extraction for 30 minutes. The operation is relatively simple, but the low water content in the extraction solvent may not be conducive to Rutin transfers, leading to low content results

Images