Preparation method of liquid feed acidulant
An acidifier and feed technology, applied in the fields of feed, enzyme preparations and microorganisms, can solve the problems that do not involve adding Chinese herbal medicines and vitamins, do not involve concentrated organic acid technology, and do not involve citric acid crystallization technology.
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Embodiment 1
[0108] A kind of preparation of composite liquid feeding acidulant comprises the following steps:
[0109] Grind 140kg of broken rice and 10kg of corn, add 1050kg of water to adjust the slurry, add 0.15-0.20kg of liquefaction enzyme and glucoamylase to liquefy and saccharify when the temperature rises to 95°C, and obtain 1200kg of slag-containing sugar liquid, sterilize and cool at high temperature, and insert 20kg of citric acid-producing strains were aerobically fermented at 22-28°C for 60-72 hours, and then 30kg of citric acid residue wet base and 1150-1165kg of citric acid fermentation broth were obtained through solid-liquid separation;
[0110] After 1150-1165kg of citric acid fermentation broth is evaporated and concentrated, 290-320kg of citric acid concentrate is prepared;
[0111] Grind 100kg of corn, add 250kg of water to make slurry, add liquefaction enzyme and glucoamylase to enzymatically hydrolyze, and obtain 350kg of saccharified liquid with slag. Through soli...
Embodiment 2
[0117] A kind of preparation of composite liquid feeding acidulant comprises the following steps:
[0118] Grind 140kg of broken rice and 10kg of corn, add 1050kg of water to adjust the slurry, add 0.15-0.20kg of liquefaction enzyme and glucoamylase to liquefy and saccharify when the temperature rises to 95°C, and obtain 1200kg of slag-containing sugar liquid, sterilize and cool at high temperature, and insert 20kg of citric acid-producing strains were aerobically fermented at 22-28°C for 60-72 hours, and then 30kg of citric acid residue wet base and 1150-1165kg of citric acid fermentation broth were obtained through solid-liquid separation;
[0119] After 1150-1165kg of citric acid fermentation broth is evaporated and concentrated, 290-320kg of citric acid concentrate is prepared;
[0120] Grind 100kg of corn, add 300kg of water to adjust the slurry, add liquefaction enzyme and glucoamylase for enzymatic hydrolysis, and obtain 400kg of saccharification liquid with slag. Thro...
Embodiment 3
[0126] A kind of preparation of lactic acid type liquid feeding acidulant comprises the following steps:
[0127] Crush 100kg of broken rice, add 400kg of water to adjust the slurry, add liquefying enzyme and glucoamylase for enzymatic hydrolysis, and obtain 500kg of saccharified liquid with slag. Through solid-liquid separation, 18kg of sugar residue wet base and 482kg of residue-free saccharification liquid were obtained.
[0128] In 482kg of slag-free saccharification liquid, high-temperature sterilization and cooling, 20kg of lactic acid-producing lactic acid bacteria seed solution was inserted, and under the basic constant conditions of continuous flow of calcium hydroxide solution, temperature 42-45°C, and fermentation pH: 7, carried out After anaerobic fermentation for 48-60 hours, 482 kg of crude lactic acid fermentation liquid was obtained, and after solid-liquid separation, lactic acid residue and lactic acid fermentation liquid were obtained. Add 60kg of 20% dilute...
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