Serratia marcescens RZ 21-C6 and application thereof

A technology of RZ21-C6 and Serratia marcescens, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problem of lack of ability to utilize straw hydrolyzate, insufficient utilization of cheap biomass carbon sources, and poor genetic stability of strains And other issues

Active Publication Date: 2016-09-28
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention intends to carry out mutagenesis breeding through normal pressure and room temperature plasma, and expects to solve the problems of poor genetic stability of the above-mentioned strains, low prodigiosin production, and insufficient utilization of cheap biomass carbon sources, especially the lack of xylose-containing The utilization capacity of a large amount of pesticide residue straw hydrolyzate

Method used

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  • Serratia marcescens RZ 21-C6 and application thereof
  • Serratia marcescens RZ 21-C6 and application thereof
  • Serratia marcescens RZ 21-C6 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] ARTP mutagenesis and screening of high prodigiosin bacterial strains of embodiment 1

[0034] (1) Strain mutagenesis: the lethality curve of the original strain of Serratia marcescens induced by plasma at normal pressure and room temperature is as follows: figure 1As shown (the original strain was isolated from a commercially available photosynthetic bacteria agent for aquatic products, numbered RZ 21, see the literature for details: Min Taoling, Yang Qiyin, Guo Xiaoxiao, etc. A strain of Serratia marcescens in the photosynthetic bacteria agent Separation and identification. Anhui Agricultural Sciences, 2006,34(22):5837-5838,5841.), plasma is relatively strong to Serratia marcescens, and it will kill nearly 60% of the thalli for 15s; After 60s, the lethal rate reaches over 90%; after 240s, the lethal rate reaches 100%. The mutation itself is random, and the relationship between its lethality and positive mutations is not very clear, but according to literature reports,...

Embodiment 2

[0038] Embodiment 2 mutant strain physiological performance analysis

[0039] (1) TEM observation of cell morphology changes: the Serratia marcescens mutant strain RZ 21-C6 was observed under a transmission electron microscope, and its morphology had significant changes compared with the starting strain RZ21 ( Image 6 ). The cell volume of the mutant bacteria is significantly larger, and the specific surface area of ​​the cells is larger; the cell wall becomes very thin, the cell surface becomes round and transparent, and the permeability increases, which will be very conducive to the secretion of intracellular secondary prodigiosin To the outside of the cell, it reduces the harmful effect on itself, and it is also beneficial to the separation and purification process of the later product.

[0040] (2) Analysis of cell membrane phospholipid fatty acid composition: As can be seen from Table 1, the fatty acid composition in the cell membrane phospholipid of the mutant strain R...

Embodiment 3

[0046] Embodiment 3 Mutant bacteria and original bacteria are compared to the utilization situation of different carbon sources

[0047] (1) Investigation of the utilization of four basic carbon sources (sucrose, glucose, xylose and arabinose) by the mutant strain RZ 21-C6 is shown in Table 2. Compared with the original strain, the production of prodigiosin in the sucrose-containing medium of the mutant strain was increased by 2.59 times, and the production efficiency was increased by 2.63 times, indicating that the screened strain not only has higher pigment production, but also has improved production efficiency , which is consistent with the conclusions drawn from the physiological changes in Example 2. In the xylose-containing medium, the amount of prodigiosin produced by RZ 21-C6 was 17.21 times higher than that of RZ 21, reaching 3.27g / L. Compared with the original strain, which cannot use glucose to produce pigment, the mutant strain RZ 21-C6 can use glucose well to pr...

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Abstract

The invention discloses serratia marcescens RZ 21-C6 and application thereof. The preservation number of the serratia marcescens RZ 21-C6 is CGMCCNo.12714. The mutant strain has the advantages that the utilization efficiency of a substrate, the genetic stability and the yield of prodigiosin are high, and the utilization effect of the mutant strain on xylose is particularly good. In a 5L fermentation tank, pesticide residue straw hydrolysate with xylose as a main component is low in price, a biomass carbon source is developed for production of the prodigiosin, the yield of the prodigiosin can reach 15-20 g / L during multi-batch fermentation, and the conversion rate of total reducing sugar directly used for prodigiosin synthesis is 20% or above. The method for producing the prodigiosin through the serratia marcescens at normal pressure and room temperature by means of plasma mutant strain and the utilization of the xylose and the pesticide residue straw hydrolysate by the serratia marcescens can promote achievement of prodigiosin industrialization, and the method can be used for the fields of biological medicines, ecological environmental management, agricultural biological controlling and the like.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for efficiently utilizing xylose or pesticide residue straw hydrolyzate to produce high prodigiosin by Serratia marcescens after plasma mutagenesis at normal pressure and room temperature. Background technique [0002] Prodigiosin is a general term for a family of natural red pigments. Its basic structure is a methoxypyrrole skeleton composed of three pyrrole rings. The molecular formula is C 20 h 25 N 3 O, the molecular weight is 323.2Da. Since Amako et al. discovered it in 1929 while studying the growth of Serratia and was first isolated by Harashima et al. in 1960, the research on the properties and biological activity of the substance has attracted much attention. In addition to being a natural biological dye, many of its biological activities have been gradually recognized, including antibacterial, antimalarial, antifungal and antiprotozoal activities. A lar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/16C12R1/43
CPCC12P17/165C12R2001/43C12N1/205
Inventor 陈飞戴传超杨晶杨启银
Owner NANJING NORMAL UNIVERSITY
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