Preparation method and application of effective parts with tyrosinase inhibitory effect in mulberry branches

A technology of tyrosinase and active parts, applied in the preparation of organic compounds, medical preparations containing active ingredients, active ingredients of ketones, etc., can solve problems such as unclear ingredients

Active Publication Date: 2019-06-07
广州箴美科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Morus twig extracts have inhibitory effects on tyrosinase and melanin synthesis in B-16 melanocytes, but so far, the components that have inhibitory effects on tyrosinase in Morus twigs have been unclear

Method used

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  • Preparation method and application of effective parts with tyrosinase inhibitory effect in mulberry branches
  • Preparation method and application of effective parts with tyrosinase inhibitory effect in mulberry branches
  • Preparation method and application of effective parts with tyrosinase inhibitory effect in mulberry branches

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation of effective fraction

[0020] 10 kg of mulberry branches were ultrasonically extracted with 2 times the amount of 70% ethanol for 3 times, 1 hour each time, concentrated to dryness under reduced pressure, and weighed. Dissolve an appropriate amount of dry matter in 95% ethanol, filter, add to macroporous resin D101, dry naturally or evaporate to dryness under reduced pressure, and then apply macroporous resin D101 chromatography column, using 20% ​​and 40% of the volume of 8 columns respectively , 60%, 80%, and 95% ethanol were eluted, the eluent was collected, and the ethanol was recovered under reduced pressure to dryness, and the 40% eluted part was detected by high performance liquid chromatography, which contained five compounds oxidized resveratrol, grass The components of halberine, sangxinin M, 2,4,2',4'-tetrahydroxychalcone and mochalcone A are effective parts ( figure 1 ).

[0021] HPLC detection conditions:

[0022] Instrument: Water...

Embodiment 2

[0025] Example 2 Preparation of oxidized resveratrol and 2,4,2',4'-tetrahydroxychalcone

[0026] The extraction of mulberry branch, macroporous resin separation process is the same as embodiment 1, collects 8 column volumes of 40% ethanol eluting part, concentrates and dries to obtain sample, weighs, separates with 200-300 mesh silica gel column chromatography, uses volume ratio respectively For 50:1 and 30:1 chloroform-methanol elution, collect the eluate obtained by 30:1 chloroform-methanol elution by volume ratio, and then use Sephadex LH-20 gel column chromatography to separate, the volume ratio 1:1 methanol-water elution, that is.

[0027] The nuclear magnetic resonance spectrum data of oxidized resveratrol are as follows:

[0028] 1 H NMR (400MHz, CD 3 OD) δ: 7.32(1H,d,J=9.1Hz,H-6),7.26(1H,d,J=16.4Hz,H-7),6.81(1H,d,J=16.4Hz,H-8 ),6.45(2H,d,J=2.0Hz,H-2',6'),6.31(2H,overlap,H-3,5),6.14(1H,t,J=2.0Hz,H-4' ). 13 C NMR (100MHz, CD 3 OD)δ:159.8(C,C-3',5'),159.4(C,C-4),15...

Embodiment 3

[0035] Example 3 Preparation of Eugenin, Sangsin M and Mochalcone A

[0036]The extraction of mulberry branch, macroporous resin separation process is the same as embodiment 1, collects 8 column volumes of 40% ethanol eluting part, concentrates and dries to obtain sample, weighs, separates with 200-300 mesh silica gel column chromatography, uses volume ratio respectively For 50:1 and 30:1 chloroform-methanol elution, collect the eluate obtained by 50:1 volume ratio of chloroform-methanol elution, and then use Sephadex LH-20 gel column chromatography to separate, the volume Methanol-water ratio 1:1 elution, that is, eugenin, sangsin M and mochalcone A.

[0037] The nuclear magnetic resonance spectrum data of eugenin are as follows:

[0038] 1 H NMR (Acetone-d 6 ,400MHz)δ:12.210(1H,OH-5),9.019(2H,OH),7.290(1H,d,J=8.4Hz,H-6'),6.465(1H,d,J=2.4Hz,H -2'),6.415(1H,dd,J=8.4,2.4Hz,H-5'),5.955(1H,d,J=2.0Hz,H-8),5.936(1H,d,J=2.0Hz ,H-6), 5.692(1H,dd,J=13.2,2.8Hz,H-2),3.163(1H,dd,J=1...

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Abstract

The invention discloses a preparation method and an application of a mulberry twig effective part with a tyrosinase inhibiting effect and belongs to the technical field of medicine and food. The mulberry twig effective part with the tyrosinase inhibiting effect contains five polyphenol compounds which are extracted from mulberry twigs and have tyrosinase inhibiting activity, and the five polyphenol compounds include oxyresveratrol, Steppogenin, Moracin M, 2,4,2',4'-tetrahydroxychalcone and mole Morachalone A. The mulberry twig effective part with the tyrosinase inhibiting effect can be effectively applied to inhibition for enzymatic browning of fruits and vegetables and skin whitening of cosmetics, used for preventing and treating human pigmentation diseases and melanoma caused by excessive melanin, and applied to various preparations of drugs for treating diseases requiring inhibition of tyrosinase activity.

Description

technical field [0001] The invention relates to a preparation method and an application of effective parts with tyrosinase inhibitory effect in mulberry branches, belonging to the technical fields of medicine and food. Background technique [0002] Sangzhi, the general term for the branches and leaves of mulberry trees, mulberry branches, mulberry sticks, and tender mulberry branches, is widely distributed and planted in most parts of my country; it is mainly produced in Jiangsu, Zhejiang, Anhui, Hunan, Hebei, Sichuan and other places. Modern pharmacological studies have shown that some components obtained from this plant have many pharmacological effects such as anti-inflammatory, anti-oxidation, and anti-cancer. The chemical components of mulberry branch include flavonoids, terpenes, stilbenes, long-chain fatty acids, etc. Morus twig extract has inhibitory effects on tyrosinase and melanin synthesis in B-16 melanocytes, but so far, the ingredients that have inhibitory eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C39/21C07C37/68C07C37/82C07D311/60C07D307/80C07C49/835C07C45/78C07C45/79A61K31/05A61K31/12A61K31/343A61K31/353A61P17/00A61P35/00A61K8/34A61K8/49A61K8/35A61Q19/02
CPCA61K8/347A61K8/35A61K8/4973A61K8/498A61K2800/782A61Q19/02C07C37/685C07C37/82C07C45/78C07C45/79C07D307/80C07D311/60C07C39/21C07C49/835
Inventor 郑宗平
Owner 广州箴美科技有限公司
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