Surface plasma resonance sensing chip and preparation method and application thereof
A surface plasmon and sensor chip technology, used in measurement devices, material analysis by optical means, instruments, etc., can solve the problems of high instrument maintenance costs, expensive CM5 chips, and long time required, and achieves short cycle times. The effect of fixed capacity and good uniformity
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preparation Embodiment 1
[0064] Such as figure 1 As shown, it is a schematic flow diagram of the preparation method of the present invention. Specifically, the preparation method of the surface plasmon resonance sensor chip of the present invention comprises the following steps:
[0065] (1) Cleaning the glass substrate: washing the glass substrate with concentrated sulfuric acid and deionized water successively, and drying;
[0066] (2) A chromium film with a thickness of 3-10nm is prepared on a glass substrate by sputtering or deposition, and then a gold film with a thickness of 45-55nm is prepared on the chromium film. The solid-phase carrier of the sensor chip is made into a bare gold chip;
[0067] (3) Drop the solution containing modified bovine serum albumin on the bare gold chip, so that the solution evenly covers the entire chip, continue to drop until the entire chip is soaked, seal the oscillation reaction, and use the self-assembly method to carry out the surface of the gold film. Chemi...
preparation Embodiment 2
[0072] The preparation method of the off-line immobilized small molecule detection SPR sensor chip also includes the following step (6), taking the cortisol chip as an example.
[0073] Described step (6) comprises following substep:
[0074] a) Add the mixed solution of NHS and EDC (1:1 mixing, the concentration of NHS 0.1mol / L, the concentration of EDC 0.4mol / L) on the blank chip, so that the solution evenly covers the entire chip, continue to drop until soaked The whole chip is sealed and oscillating, so that the modified bovine serum albumin immobilized on the surface of the gold film undergoes a chemical cross-linking reaction, and reacts at room temperature for 15 minutes to 1 hour, and then cleans and dries the chip;
[0075] b) Drop a cortisol conjugate solution with 0.1M acetic acid buffer as a solvent on the chip, the concentration of the conjugate is 300mg / L, so that the solution evenly covers the entire chip, continue to drop until the entire chip is soaked, seal a...
preparation Embodiment 3
[0078] The preparation method of the online immobilized small molecule detection SPR sensor chip also includes the following step (6), taking the cortisol chip as an example.
[0079] Described step (6') comprises following sub-steps:
[0080] a') Install the blank chip on the surface plasmon resonance biochemical analyzer, and fix the supporting flow cell;
[0081] b') flow into the mixed solution of NHS and EDC (1 to 1 mixing, the concentration of NHS 0.1mol / L, the concentration of EDC 0.4mol / L), so that the modified bovine serum albumin immobilized on the surface of the gold film undergoes chemical For cross-linking reaction, react at room temperature for 7 minutes to 0.5 hours, and then wash with PBS buffer;
[0082] c') flow into the cortisol conjugate solution with 0.1M acetate buffer as solvent, the concentration of the conjugate is 100mg / L, react at room temperature for 10 minutes to 1 hour, and then flow wash with PBS buffer;
[0083] d') Continuously and slowly flo...
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