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Milt DNA-NA, protamine extract and preparing method thereof

A technology of fish testis extract and protamine, applied in the field of biomedicine, can solve the problems of using toxic substances and the like, and achieve the effects of good antibacterial properties, high yield and purity, good social benefits and economic benefits

Active Publication Date: 2016-10-19
SHANGHAI HUIWEN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects that the existing protamine and protamine DNA preparation methods can only extract one of them, and use toxic substances in the extraction process, and provide protamine DNA-NA Extract, protamine extract, method for its preparation and method for preparing both simultaneous extractions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 prepares the extract of protist DNA-NA

[0040] (1) Take the fish testis, add water to wash, drain the water, and obtain the cleaned fish testis raw material;

[0041] (2) Add 3 times the volume of water to the fish testis raw material obtained in step (1), and add 0.5% alkaline protease to enzymolyze it for 240 minutes at 56° C. to obtain the fish testis raw material after enzymolysis. The percentage It is the mass percentage that accounts for fish testis raw material;

[0042] (3) Filtrating the enzymolyzed fish testis raw material obtained in step (2), and concentrating the filtrate obtained by filtration to 50% of the original volume of the filtrate, which is feed liquid A;

[0043] (4) adjust the pH value of the feed liquid A obtained in step (3) to 6.5 to obtain feed liquid B, put the cation exchange resin column, collect the effluent, and wash the cation exchange resin column with water, combine the effluent and eluent , concentrate it to 15% of th...

Embodiment 2

[0046] Embodiment 2 prepares protamine extract

[0047] (1) Take the fish testis, add water to wash, drain the water, and obtain the cleaned fish testis raw material;

[0048] (2) Add 5 times the volume of water to the fish testis raw material obtained in step (1), and add 0.6% compound flavor protease for enzymolysis at 45°C for 60 minutes to obtain the fish testis raw material after enzymolysis, the percentage It is the mass percentage that accounts for fish testis raw material;

[0049] (3) Filtrating the fish testis raw material after enzymolysis obtained in step (2), and concentrating the filtrate obtained by filtration to 25% of the original volume of the filtrate, which is feed liquid A;

[0050] (4) adjust the pH value of the feed liquid A obtained in step (3) to 7.5 to obtain feed liquid B, put the cation exchange resin column, collect the effluent, and wash the cation exchange resin column with water, combine the effluent and eluent , concentrate it to 50% of the o...

Embodiment 3

[0054] Embodiment 3 prepares the extract of protamine DNA-NA and protamine protein extract

[0055] (1) Take the fish testis, add water to wash, drain the water, and obtain the cleaned fish testis raw material;

[0056] (2) Add 10 times the volume of water to the fish testis raw material obtained in step (1), and add 0.5% trypsin to enzymolyze it for 300 minutes at 58° C. to obtain the fish testis raw material after enzymolysis. The percentage is: Accounting for the mass percentage of fish testis raw material;

[0057] (3) Filtrating the fish testis raw material after enzymolysis obtained in step (2), and concentrating the filtrate obtained by filtration to 10% of the original volume of the filtrate, which is feed liquid A;

[0058] (4) adjust the pH value of the feed liquid A obtained in step (3) to 7 to obtain feed liquid B, put the cation exchange resin column, collect the effluent, and wash the cation exchange resin column with water, combine the effluent and eluent , co...

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Abstract

The invention discloses a protamine DNA-NA extract, a protamine protein extract and a preparation method thereof. The method is as follows: (1) take the fish testis, add water to wash it, drain the water, and obtain the raw material of the fish testis; (2) add 1 to 10 times the volume of water, and at a temperature of 37 to 58 ° C, add 0.1 to 1.2%, enzymatic hydrolysis for 30 to 300 minutes; (3) Concentrate the filtrate to 30 to 80%; (4) Adjust the pH to 6.0 to 8.0 and put it on a cation exchange resin column, collect the effluent, and combine the combined effluent and eluate (5) Use 1-5% sodium chloride solution to continue eluting the cation exchange resin until the effluent has no protein, and collect the eluate. The protamine extract and protamine DNA-NA extract have high yield and purity, can be obtained simultaneously, and do not use polluting and toxic substances.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to protamine DNA-NA, protamine extract and a preparation method thereof. Background technique [0002] The testis tissue of mature male fish is called mint essence, which is mostly discarded or used as waste because of its peculiar smell. However, fish testis contains a large amount of protamine and protamine nucleic acid substances, both of which have good effects. Protamine is a kind of basic protein, in which the content of arginine is relatively high. Arginine can strengthen liver function, stimulate the pituitary gland to release gonadotropin, and can treat male infertility. At the same time, protamine has broad-spectrum antibacterial activity and is a natural preservative. Because protamine is completely a natural component, it has high safety and has the advantages of safety, non-toxicity and no side effects. As a new type of Preservatives have broad application prospects in the ...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/60A61K8/44A61K8/64A61Q19/00A61Q19/08
Inventor 骆峰周自华杨海延
Owner SHANGHAI HUIWEN BIO TECH
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