A kind of bacterial strain and synthetic method utilizing chitin to synthesize pha and application thereof

A chitin and strain technology, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high cost of bioplastics, environmental pollution, etc., and achieve low water treatment costs and reduced production. Effects on cost, broad industry and development prospects

Active Publication Date: 2019-09-27
李涛 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a technology to synthesize PHA by using the enzymatic hydrolysis product after chitin degradation as the carbon source of PHA-producing Pseudomonas YJY25, and to obtain degradable bioplastics through a low-cost method, which solves the current problem. Refractory plastics cause great pollution to the environment and the current situation of high cost of biodegradable bioplastics, and use them in the denitrification treatment process of wastewater, and rationally use chitin, a low-cost, renewable biological resource

Method used

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  • A kind of bacterial strain and synthetic method utilizing chitin to synthesize pha and application thereof

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Embodiment 1

[0020] A method for synthesizing PHA using chitin, the specific operation method includes: (1) preparing chitin into a colloidal solution, and adjusting the pH value to 6.0, adding 5% compound enzyme preparation, and performing enzyme degradation on a shaking table at 60°C, The degradation time is 8 hours, and the degradation product is flocculated, precipitated, and filtered to obtain the enzymatic hydrolysis product; (2) Pseudomonas YJY25 is activated in the seed medium, the activation condition is 30°C, the shaking table is 200r / min, and the activation is 48h; After activation, the bacterial liquid is inserted into the fermentation culture with an inoculum of 1.0% (v%) for cultivation, and then inoculated into the medium with the enzymatic hydrolysis product obtained in step (1) as the only carbon source for cultivation. The conditions are temperature 35°C, pH 7.0, and culture time 48 hours; (3) The culture (that is, the fermentation product) obtained in step (2) undergoes c...

Embodiment 2

[0023] A method for synthesizing PHA from chitin. The specific operation method includes: (1) preparing chitin into a colloidal solution, adjusting the pH value to 5.0, adding 15% compound enzyme preparation, and performing enzyme degradation on a shaking table at 50°C. The degradation time is 10 hours, and the degradation product is flocculated, precipitated, and filtered to obtain the enzymatic hydrolysis product; (2) Pseudomonas YJY25 is activated in the seed medium, the activation condition is 35°C, the shaking table is 250r / min, and the activation is 24h; After activation, the bacterial solution was inserted into the fermentation culture with an inoculum of 2.0% (v%) for cultivation, and then inoculated into the culture medium with the enzymatic hydrolysis product obtained in step (1) as the only carbon source for cultivation. The conditions are temperature 30°C, pH 7.2, and culture time 50 hours; (3) The culture obtained in step (2) undergoes cell wall crushing, flocculat...

Embodiment 3

[0026] A method for synthesizing PHA using chitin, the specific operation method includes: (1) preparing chitin into a colloidal solution, and adjusting the pH value to 6.5, adding 20% ​​compound enzyme preparation, and performing enzyme degradation on a shaking table at 35°C, The degradation time was 1 hour, and the degradation product was flocculated, precipitated, and filtered to obtain the enzymatic hydrolysis product; (2) Pseudomonas YJY25 was activated in the seed medium, the activation condition was 32°C, the shaking table was 230r / min, and the activation was 35 hours; After activation, the bacterial solution was inserted into the fermentation culture with an inoculum of 1.5% (v%) for cultivation, and then inoculated into the medium with the enzymatic hydrolyzate obtained in step (1) as the only carbon source for cultivation. The conditions are temperature 25°C, pH 7.5, and culture time 60 hours; (3) The culture obtained in step (2) undergoes cell wall crushing, floccula...

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Abstract

The invention relates to a bacterial strain that utilizes chitin to synthesize PHA, Pseudomonas YJY25, the preservation number is: CCTCC M 2016075, and the preservation time is: 2016.02.28; the synthesis method includes using chitin compound enzyme to degrade chitin to form an enzymatic hydrolysis product Steps, the step of cultivating Pseudomonas YJY25 with the enzymatic hydrolysis product as a carbon source, and the step of collecting the fermentation product of Pseudomonas YJY25 to obtain PHA; PHA can also be used for wastewater treatment by synthesizing water treatment preparations in addition to being used as bioplastics. Obtaining degradable bioplastics through a low-cost method solves the current situation that refractory plastics cause great pollution to the environment and the cost of degradable bioplastics is high, and it is used in the denitrification treatment process of wastewater, and the Reasonable utilization of chitin, a low-cost, renewable biological resource.

Description

technical field [0001] The invention relates to the use of complex enzymes to degrade chitin and use it as a carbon source for polyhydroxyalkanoates (PHA) synthetic bacteria to ferment and synthesize PHA. It relates to the technical field of microbial fermentation and is a secondary optimization of low-cost biological resources. Background technique [0002] At present, plastics produced from petroleum have been widely used in various industries. However, the "white pollution" and garbage siege caused by discarded plastics, as well as the air pollution and water pollution caused by plastic burning have seriously affected people's living environment. With the continuous growth of plastic production and the expansion of application fields, the pollution of plastic waste to the environment is also becoming more and more serious. As one of the important measures to protect the environment, the development of degradable plastics has become a hot issue in the world. Biodegradable...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/62C02F1/00C12R1/38
CPCC02F1/00C02F2209/06C02F2209/14C02F2209/16C12P7/625C12N1/205C12R2001/38
Inventor 李涛姚嘉赟
Owner 李涛
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