Mixed cell preparation and preparation method and application thereof

A cell preparation and cell technology, applied in the field of immune cells, can solve the problems of limited source of islets, inability to completely solve islet β cells, and high cost, so as to reduce or eliminate attacks on islet β cells, alleviate or treat type 1 diabetes, Promotes regenerative and repairing effects

Inactive Publication Date: 2016-11-09
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment of diabetes mainly relies on drugs and insulin to control blood sugar. Diabetes cannot be cured. Patients need to use drug treatment from the onset of onset, and need to take hypoglycemic drugs and/or insulin injections for life, which is expensive
Although insulin therapy can effectively alleviate the symptoms of diabetes, insulin therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mixed cell preparation and preparation method and application thereof
  • Mixed cell preparation and preparation method and application thereof
  • Mixed cell preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] The present invention also provides a method for preparing the above-mentioned mixed cell preparation, the preparation method includes the following steps:

[0043] S1. Obtain the above-mentioned mesenchymal stem cells;

[0044] S2. Acquiring the above-mentioned regulatory T cells;

[0045] S3. The above solution is provided, and mesenchymal stem cells and regulatory T cells are added to the solution.

[0046] The preparation method of the mixed cell preparation of the present invention is simple and efficient, and the preparation can be completed only by blending mesenchymal stem cells, regulatory T cells and an appropriate amount of solution. It should be noted that the mesenchymal stem cells can be obtained by ex vivo tissue culture, or by purchasing commercially available mesenchymal stem cells; the regulatory T cells can be obtained by separating peripheral blood, or by purchasing commercially available Regulatory T cells are obtained.

[0047] Further, this step ...

Embodiment 1

[0056] 1. Obtain human umbilical cord mesenchymal stem cells

[0057] (1) Isolation of primary umbilical cord mesenchymal stem cells

[0058] After the human umbilical cord was removed from the operating table, it was immersed in physiological saline containing antibiotics and stored at 4°C;

[0059] Take out the human umbilical cord in the ultra-clean bench, rinse the residual blood in the umbilical artery and umbilical vein with D-PBS buffer solution, use hemostatic forceps and scissors to remove the washed arteries and veins, and then cut the human umbilical cord into 1mm 3 After the size of the tissue block, put it into a 200ml blue cap reagent bottle;

[0060] Add 30ml of type II collagenase with a mass volume ratio of 0.1% to the blue cap reagent bottle, place the blue cap reagent bottle in a constant temperature shaker for 6 hours, and then filter and collect primary umbilical cord mesenchymal stem cells with a 100-mesh sieve ;

[0061] (2), culture of primary umbil...

Embodiment 2

[0084] 1. Obtain human umbilical cord mesenchymal stem cells

[0085] Please refer to Example 1, which is the same as Example 1.

[0086] 2. Obtain regulatory T cells

[0087] Please refer to Example 1, which is the same as Example 1.

[0088] 3. Preparation of mixed cell preparations

[0089] Provide human umbilical cord mesenchymal stem cells 8×10 6 , CD4 + CD25 + Treg cells 1×10 7 One, and solution 20ml, wherein, this solution comprises the glucose that mass fraction is 5%, the NaCl that mass fraction is 0.45%, the human serum albumin that mass fraction is 0.5% and the water that mass fraction is 94.05%; Mesenchymal stem cells, CD4 + CD25 + Treg cells are mixed with the solution to prepare a fresh non-refrigerated cell suspension. In this mixed cell preparation, the concentration of human umbilical cord mesenchymal stem cells was 4×10 5 cells / ml, CD4 + CD25 + The concentration of Treg cells was 0.5×10 6 pieces / ml.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a mixed cell preparation and a preparation method and application thereof. The mixed cell preparation comprises mesenchymal stem cells, regulatory T cells and a solution; in the mixed cell preparation, the concentration of the mesenchymal stem cells is (1-10)*10<5>/ml, and the concentration of the regulatory T cells is (0.1-3)*10<6>/ml. The mixed cell preparation can promote regeneration and repair of pancreatic beta cells and inhibit damage of an autoimmunity reaction to the pancreatic beta cells, therefore, the mixed cell preparation can treat diabetes mellitus type 1, and pancreas islet transplantation is not needed.

Description

technical field [0001] The invention relates to the technical field of immune cells, in particular to a mixed cell preparation and its preparation method and application. Background technique [0002] Diabetes is a chronic disease with a large number of patients, great harm and slow progress. Among them, type 1 diabetes (T1DM) is an autoimmune disease mediated by T cells. Under the interaction of genetic susceptibility factors and environmental factors, the immune regulation in the body is gradually unbalanced, and it is characterized by severe insulin secretion disorders caused by pancreatic β-cell damage. , blood sugar continued to rise as a sign. [0003] At present, the treatment of diabetes mainly relies on drugs and insulin to control blood sugar. Diabetes cannot be cured. Patients need to use drug treatment from the onset of onset, and need to take hypoglycemic drugs and / or insulin injections for life, which is expensive. Although insulin therapy can effectively all...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/28A61P3/10A61P37/02A61K35/17
CPCA61K35/28A61K35/17A61K2300/00
Inventor 曾宪卓唐熙
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap