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Culture method of artificial liver repair material based on adult stem cell-regenerated silk fibroin tissue engineering scaffold

A technology for regenerating silk fibroin and tissue engineering scaffolds, applied in the field of artificial liver, can solve the problems of horizontal comparison and systematic research of ex vivo liver-like cells, and achieve the effects of excellent mechanical properties, short induction time and good induction effect.

Active Publication Date: 2016-11-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for many stem cells derived from adult tissues, no one has done a horizontal comparison and systematic study of their ability to form hepatic-like cells in vitro

Method used

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  • Culture method of artificial liver repair material based on adult stem cell-regenerated silk fibroin tissue engineering scaffold
  • Culture method of artificial liver repair material based on adult stem cell-regenerated silk fibroin tissue engineering scaffold

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Embodiment 1

[0041] A method for culturing artificial liver repair materials based on adult stem cell-regenerated silk fibroin tissue engineering scaffold. First, prepare adult stem cell-regenerated silk fibroin tissue engineering scaffold for bioartificial liver, and the steps are as follows:

[0042] 1) After degumming the silkworm cocoons with a 0.3% sodium carbonate aqueous solution by mass, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.0 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution. This solution is centrifuged, filtered, dialyzed and dissolved. Concentrate to obtain a 30% mass percent regenerated silk fibroin aqueous solution;

[0043] 2) Prepare HGF, FGF4 factor and bovine serum albumin aqueous solution with water as solvent, wherein the mass concentration of HGF and FGF4 factor are both 15μg / mL, and the mass concentration of bovine serum albumin is 0.6mg / mL;

[0044] 3) Mix the regenerated silk fibroin aqueous solut...

Embodiment 2

[0065] A method for culturing artificial liver repair materials based on adult stem cell-regenerated silk fibroin tissue engineering scaffold. First, prepare adult stem cell-regenerated silk fibroin tissue engineering scaffold for bioartificial liver, and the steps are as follows:

[0066] 1) After degumming the tussah cocoons with a sodium carbonate aqueous solution of 0.4% by mass, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.1 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution. This solution is centrifuged, filtered, dialyzed and Concentrate to obtain a 32% mass percent regenerated silk fibroin aqueous solution;

[0067] 2) Prepare HGF, FGF4 factor and bovine serum albumin aqueous solution with water as solvent, wherein the mass concentration of HGF and FGF4 factor are both 16μg / mL, and the mass concentration of bovine serum albumin is 0.7mg / mL;

[0068] 3) Mix the regenerated silk fibroin aqueous solution with H...

Embodiment 3

[0077] A method for culturing artificial liver repair materials based on adult stem cell-regenerated silk fibroin tissue engineering scaffold. First, prepare adult stem cell-regenerated silk fibroin tissue engineering scaffold for bioartificial liver, and the steps are as follows:

[0078] 1) After degumming castor cocoons with 0.5% by mass sodium carbonate aqueous solution, they are dissolved in a 9.2mol / L lithium bromide aqueous solution to obtain a regenerated silk fibroin-lithium bromide aqueous solution, which is centrifuged, filtered and dialyzed And concentration to obtain a 34% regenerated silk fibroin aqueous solution;

[0079] 2) Prepare HGF, FGF4 factor and bovine serum albumin aqueous solution with water as solvent, wherein the mass concentration of HGF and FGF4 factor are both 18μg / mL, and the mass concentration of bovine serum albumin is 0.8mg / mL;

[0080] 3) Mix the regenerated silk fibroin aqueous solution with HGF, FGF4 factor and bovine serum protein aqueous solutio...

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Abstract

The invention relates to a culture method of an artificial liver repair material based on an adult stem cell-regenerated silk fibroin tissue engineering scaffold. The method mainly comprises two steps: first step, the adult stem cell-regenerated silk fibroin tissue engineering scaffold which is mixed with HGFs (hepatocyte growth factors), FGF4 (fibroblast growth factors 4), bovine serum albumin and a regenerated silk fibroin material and applied to a bioartificial liver is prepared with an electrostatic spinning method; second step, the artificial liver repair material is cultured on the basis of the adult stem cell-regenerated silk fibroin tissue engineering scaffold, and ADSCs (adipose tissue-derived stromal cells) or BMSCs (bone mesenchymal stem cells) adhere to and proliferate on the regenerated silk fibroin material and are induced to form liver-like cells under the action of scaffold sustained-release HGFs and FGF4. The prepared artificial liver repair material based on the adult stem cell-regenerated silk fibroin tissue engineering scaffold has good biocompatibility, excellent biodegradability and excellent mechanical property and is a good alternative scaffold material for the bioartificial liver.

Description

Technical field [0001] The invention belongs to the technical field of artificial liver, and relates to a method for culturing artificial liver repair materials based on adult stem cells-regenerated silk fibroin tissue engineering scaffold. Background technique [0002] my country is a big country with hepatitis B, and the hepatitis virus carrier rate is as high as 10%. With economic development and lifestyle changes, alcoholic liver disease caused by heavy drinking is also on the rise year by year; with antibiotics, weight loss drugs and health care The abuse of drugs and drug-induced liver disease are also increasing year by year. These huge numbers of patients with liver disease, after a certain course of disease, some patients will progress to end-stage liver disease or acute liver failure. Orthotopic liver transplantation is the only effective treatment for this type of disease, but due to many factors such as lack of donor liver, immune rejection, surgical risk, high cost, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/22
CPCA61L27/22A61L27/3834A61L2300/252A61L2430/28
Inventor 张耀鹏阎丽徐丽娟黄利邵惠丽胡学超
Owner DONGHUA UNIV
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