Maize straw pretreatment process adopting multi-hydrogen-bond-donor deep-eutectic solvent and recycling method of multi-hydrogen-bond-donor deep-eutectic solvent

A technology of deep eutectic solvent and corn stalks, which is applied in the field of chemical engineering, can solve the problems of low toxicity and biodegradability, and achieve the effect of process economy, less three wastes, and good pretreatment effect

Inactive Publication Date: 2016-11-09
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the "green aspect" of ionic liquids has recently been greatly questioned, ...

Method used

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  • Maize straw pretreatment process adopting multi-hydrogen-bond-donor deep-eutectic solvent and recycling method of multi-hydrogen-bond-donor deep-eutectic solvent
  • Maize straw pretreatment process adopting multi-hydrogen-bond-donor deep-eutectic solvent and recycling method of multi-hydrogen-bond-donor deep-eutectic solvent
  • Maize straw pretreatment process adopting multi-hydrogen-bond-donor deep-eutectic solvent and recycling method of multi-hydrogen-bond-donor deep-eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Choline chloride, aspartic acid and glutamic acid pretreated corn stalks in different molar ratios

[0015] Get the choline chloride of 100g different molar proportions: aspartic acid: glutamic acid (2:0.5:0.5,2:1:0.5,2:0.5:1,2:1:1 and 2:1.5:1.5 ), adding 5 g of corn stalks, stirring at 130° C. for 2 h, then centrifuging the mixture, and washing the precipitate with deionized water for 3 times. Add pH 4.8, 50mM citric acid-sodium citrate buffer to the dried precipitate to form a mixed system, the final mass concentration of corn stalks is 10%, add The cellulase was used for enzymolysis, and the enzymolysis was carried out on a water bath shaker at 50°C and 150rpm for 72 hours, and the glucose yield was measured. The results are shown in Table 1.

[0016] Table 1 The optimization results of the molar ratio of choline chloride, aspartic acid and glutamic acid

[0017]

Embodiment 2

[0018] Example 2 Optimization of the Mass Ratio of Multi-Hydrogen Bond Donor Deep Eutectic Solvent and Corn Stalk

[0019] Get 100g of choline chloride with a molar ratio of 2:1:1: aspartic acid: glutamic acid, add corn by mass ratio 7:1, 10:1, 15:1, 20:1 and 30:1 respectively The straw was stirred at 130° C. for 2 h, then the mixture was centrifuged, and the precipitate was washed 3 times with deionized water. Add pH 4.8, 50mM citric acid-sodium citrate buffer to the dried precipitate to form a mixed system, the final mass concentration of corn stalks is 10%, add The cellulase was used for enzymolysis, and the enzymolysis was carried out on a water bath shaker at 50°C and 150rpm for 72h, and the glucose yield was measured. The results are shown in Table 2.

[0020] Table 2 Optimization results of the mass ratio of multi-hydrogen bond donor deep eutectic solvent to corn stover

[0021]

Embodiment 3

[0022] Example 3 Recovered multi-hydrogen bond donor deep eutectic solvent pretreatment of corn stalks

[0023] Take 100g of choline chloride:aspartic acid:glutamic acid with a molar ratio of 2:1:1, add 5g of corn stalks, stir at 130°C for 2h, then centrifuge the mixture, take the supernatant, and add corn stalks Straw (the mass ratio of supernatant to corn stalk is 20:1), stirred at 130° C. for 2 hours, and the above steps were repeated. All precipitates were washed 3 times with deionized water. Add pH 4.8, 50mM citric acid-sodium citrate buffer to the dried precipitate to form a mixed system, the final mass concentration of corn stalks is 10%, add The cellulase was used for enzymolysis, and the enzymolysis was carried out at 50°C and 150rpm on a water bath shaker for 72h. The choline chloride recovered four times: aspartic acid: glutamic acid pretreated corn stalks had a glucose yield of 65.4%. The results are shown in Table 3.

[0024] Table 3 Results of pretreatment of...

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Abstract

The invention relates to a maize straw pretreatment process adopting a multi-hydrogen-bond-donor deep-eutectic solvent and a recycling method of the multi-hydrogen-bond-donor deep-eutectic solvent, belonging to the field of chemical engineering. In a multi-hydrogen-bond-donor deep-eutectic solvent system composed of choline chloride, aspartic acid and glutamic acid, adding maize straw and pretreating the maize straw for 0.5-3 h at 90-130 DEG C, centrifuging the mixture, collecting the supernate, and washing the sediment for three times by adopting deionized water; adding a certain amount of maize straw into the supernate, carrying out pretreatment for certain time at certain temperature, and repeating the above steps; mixing the pretreated maize straw and citric acid-sodium citrate buffer solution at a certain solid-to-liquid ratio, and adding with cellulose for enzymolysis, wherein the final yield of glucose is greater than 76%, and after the multi-hydrogen-bond-donor deep-eutectic solvent is recycled for 4 times, the yield of glucose is greater than 65%. The process and the recycling method have the advantages that the process is simple, the efficiency is high, the procedure is economical, the discharge of industrial wastewater, waste gases and residues is little, etc., and the maize straw hydrolysate after pretreatment can be used for fermentation production of butanol.

Description

technical field [0001] The invention discloses a method for pretreating corn stalks with a multi-hydrogen bond donor deep eutectic solvent and a recycling method thereof, belonging to the field of chemical engineering. Background technique [0002] my country is a large agricultural country with rich lignocellulose raw materials. The annual output of crop straw alone is 700 million tons, accounting for about 30% of the world's total straw, equivalent to about 350 million tons of standard coal. Crop straw is rich in cellulose and hemicellulose, and is a valuable renewable resource. However, in our country, quite a lot of crop straw is discarded or burned, and has not been rationally utilized. According to reports, the utilization rate of straw in my country is about 33%, most of which have not been treated, and only 2.6% have been used after treatment. Therefore, if these crop straw resources can be comprehensively utilized, it will not only increase farmers' income, but als...

Claims

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Application Information

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IPC IPC(8): C12P19/02
CPCC12P19/02C12P2201/00
Inventor 倪晔许国超丁纪财董晋军韩瑞枝
Owner JIANGNAN UNIV
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