Construction of autologous tissue engineering blood vessel by using large-aperture parallel polycaprolactone electrospun cotton

Abstract

The invention relates to large-aperture parallel polycaprolactone (PCL) electrospun cotton, and belongs to the technical field of biomedical engineering. The invention further provides a tissue blood vessel engineering scaffold and a tissue engineering blood vessel prepared by the electrospun cotton. The construction of the autologous tissue engineering blood vessel by using the large-aperture parallel PCL electrospun cotton has the advantages that (1) the large-aperture parallel PCL electrospun cotton is good in mechanical property and cell compatibility, and is conducive to rapid ingrowth of cells; (2) finally obtained tissue engineering vascular tissue cells and extracellular matrixes derive from autografts, thereby not producing immunological rejection; (3) PCL electrospun cotton fiber guide cells which are arranged in parallel and extracellular matrixes are in a directional circular arrangement, so that histological structures of natural blood vessels can be simulated.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular, utilizes large-diameter parallel polycaprolactone electrospun cotton to construct autologous tissue engineering blood vessels. Background technique [0002] Several existing vascular graft substitutes still have defects such as non-growth, or allogeneic tissues are prone to immune rejection. Therefore, it is of great significance to construct new tissue-engineered blood vessels with growth properties and autologous tissues or cells. [0003] Generally, the construction of tissue engineered blood vessels first needs to solve three problems: (1) tissue scaffold, (2) source of seed cells and (3) tissue construction method. The "tissue scaffold" is the environment in which cells attach and grow. Since the artificially constructed tissue still lacks the extracellular matrix secreted by the implanted cells in the early stage of formation, it is necessary to artificially ...

AI Technical Summary

Problems solved by technology

However, this also brings about additional problems: (1) The process of in vitro isolation and culture expansion of seed cells is complicated, and cell contamination and aging are prone to occur; After curly transplantation, the seed cells first need to overcome the subcutaneous nutrient deficient environment, which brings about the problem of low construction success rate; (3) Cells in vitro are also prone to cell loss

[0007] The present invention hopes to solve the problem that the electrospun nanofiber membrane spun by the traditional method is fine and compact, and the cells are not easy to grow into

The large-pore size, thick fiber PCL electrospun cotton with PCL as the basic raw material and its application in autologous tissue engineering blood vessels have not been reported yet.

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