Micro-interface hydroxyl free radical characterization method based on fluorescence imaging analysis

A fluorescence imaging and free radical technology, which is applied in the direction of material analysis by optical means, analysis of materials, fluorescence/phosphorescence, etc., can solve the problems of large influence of the instrument, low oxidation selectivity, and wrong evaluation of the catalytic ability of the surface of the material, etc. Achieve the effect of strong anti-interference and simple operation steps

Inactive Publication Date: 2016-11-16
TIANJIN CHENGJIAN UNIV
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Problems solved by technology

[0003] At present, there are still great difficulties in the detection of HO intermediates, because HO itself is extremely unstable, and its oxidation selectivity is low, resulting in a very short lifetime in water.
Even the relatively high-end detection methods (such as DMPO capture combined with paramagnetic resonance detection method) still have the following two problems: (1) cannot effectively distinguish the distribution state and relative intensity of HO in the surface layer of the material and the liquid phase body, on the one hand, it is easy to cause The wrong evaluation of the contact catalytic ability of the surface layer of the material, on the other hand, will cause the wrong judgment of the main reaction "place" in the dynamic system; (2) only qualitative characterization results can be obtained through signal intensity comparison, resulting in different or similar reactions The systems are not comparable, and the results are greatly affected by the instrument

Method used

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  • Micro-interface hydroxyl free radical characterization method based on fluorescence imaging analysis
  • Micro-interface hydroxyl free radical characterization method based on fluorescence imaging analysis
  • Micro-interface hydroxyl free radical characterization method based on fluorescence imaging analysis

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 characterizes the catalytic oxidation performance of carbon nanotubes

[0033] figure 2 It is a fluorescence micrograph of carbon nanotubes as a solid-phase catalyst. From figure 2 a It can be observed that there is a highly recognizable blue area around the carbon nanotubes, indicating that a high-intensity catalytic ozone reaction has occurred on the surface of the carbon nanotubes, and a large number of hydroxyl radicals (concentration is about 60 μM) have been generated. From figure 2 b is the picture of carbon tubes under increased magnification. It can be clearly seen that there is a higher intensity cyan free radical area (concentration is about 110 μM) in the blue area, reflecting that the carbon tube surface catalyzes ozone in situ to generate a large amount of HO. Then it concentrates and diffuses to a certain extent in a very small interface area. figure 2 c is a sample diagram of the catalytic ozone reaction in a strong alkaline solution...

Embodiment 2

[0034] Embodiment 2 characterizes the catalytic oxidation performance of graphite, activated carbon and iron oxide

[0035] image 3 a is a fluorescence microscopic image of graphite as a solid-phase catalyst. It can be observed that graphite has a free radical generation state similar to that of carbon nanotubes, that is, the cyan region of the inner layer is a highly concentrated state of free radicals, and the blue region of the outer layer is Radical condensed states at solid-liquid interfaces. In contrast, the catalytic ozone ability of activated carbon decreased significantly ( image 3 b) On the one hand, the blue light intensity on the surface is obviously weakened, indicating that the amorphous carbon structure has a relatively weak ability to catalyze ozone to generate HO. On the other hand, the uneven color distribution reflects the arrangement of catalytic functional groups on the surface of amorphous carbon uneven. Iron oxide is also one of the emerging catalyt...

Embodiment 3

[0036] Example 3 comparative characterization research on the catalytic oxidation performance of carbon materials

[0037] Figure 4 It is a 400-fold magnified fluorescence micrograph of catalytic materials in an ozone environment under the conditions of parallel catalytic experiments. The comparison results clearly show that carbon nanotubes ( Figure 4 a) and graphite ( Figure 4 b) Both can rapidly promote the conversion of ozone on the surface to generate HO. In contrast, although activated carbon can also show a certain catalytic effect, the fluorescence intensity reflecting free radicals is significantly weaker. Compared Figure 4 a and Figure 4 b. Carbon nanotubes have more efficient catalytic properties. On the one hand, they have an active surface layer of graphene, and on the other hand, they are highly stable mesoporous materials, and their mass transfer and contact are significantly better than graphite. The results of this fluorescence imaging analysis furthe...

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Abstract

The invention belongs to the field of advanced oxidation water pollution control, and relates to a micro-interface hydroxyl free radical characterization method based on fluorescence imaging analysis. According to the present invention, the HO. generation intensity on the micro-interface of a material is utilized by using a fluorescence microscopy imaging analysis technology; coumarin molecules rapidly act with HO. according to a dose ratio of 1:1 to convert into heptahydroxycoumarin with fluorescent luminescent property, and the research results of the Japanese team demonstrate that the concentration of the heptahydroxycoumarin is closely related to the coloration characteristic and the intensity of the fluoresce in a specific wavelength region; an excess amount of the coumarin is added to a heterogeneous reaction system as a HO. trapping agent, such that the intensity information of the HO. on the material surface layer can be effectively and indirectly acquired by using a fluorescence microscopy colorimetry method; and with the method, the semi-quantitative detection of the HO. at the solid-liquid micro-interface in the heterogeneous dynamic catalytic system can be achieved, the catalysis function of the function material can be directly identified and expressed in the imaging manner, and the method has characteristics of strong anti-interference and relatively simple operation steps .

Description

technical field [0001] The invention belongs to the field of pollution control of advanced oxidized water, and relates to a method for characterizing hydroxyl free radicals at micro-interfaces based on fluorescence imaging analysis. technical background [0002] Heterogeneous catalytic oxidation is an emerging water treatment process based on advanced oxidation control. Through the surface catalysis of functional materials in a specific reaction system, a strong oxidative intermediate - hydroxyl radical (HO ) is generated in situ, and its electrophilic effect can deeply oxidize and degrade trace persistent organic compounds remaining in water, which has good The application prospects of environmental water treatment (such as drinking water purification, decolorization, deodorization and disinfection of reclaimed water, etc.). Controlling and strengthening the generation efficiency of HO is an important aspect of improving the water treatment capacity of heterogeneous cataly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 张硕郑剑锋孙力平臧春元樊雪峰
Owner TIANJIN CHENGJIAN UNIV
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