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Multilevel nano gold flower, its preparation method and application

A nano-gold and nano-technology, applied in the field of multi-level structure nano-gold, can solve the problems of small specific surface area, inconspicuous multi-level structure, poor repeatability, etc. The effect of increased sensitivity

Active Publication Date: 2018-02-27
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the specific surface area of ​​the nano-golden flower prepared by the existing method is relatively small, the multi-level structure is not obvious, the synthesis steps are many, and the reproducibility is poor.

Method used

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  • Multilevel nano gold flower, its preparation method and application
  • Multilevel nano gold flower, its preparation method and application
  • Multilevel nano gold flower, its preparation method and application

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preparation example Construction

[0038] In a more typical embodiment, a method for preparing nano gold flowers may include the following steps:

[0039] 1) Sodium citrate reduces chloroauric acid to prepare nano-gold sphere particles with a particle size of 10 nm to 30 nm, which are used as seeds for preparing nano-gold flower particles.

[0040] 2) Add chloroauric acid into polydiallyldimethylammonium chloride (PDDA) aqueous solution, stir at 25°C for more than 15 minutes, until the solution turns yellow, then quickly add ascorbic acid aqueous solution into the above yellow solution, and stir vigorously For more than 30 seconds, add the aforementioned gold nanosphere seeds, stir rapidly for more than 10 seconds, and stand at room temperature for more than 4 hours.

[0041] Another aspect of the present invention provides the use of the multi-level golden nano particles. For example, it is used as a gold-labeled antibody for immunochromatographic test strips in the fields of biological and chemical detection...

Embodiment 1

[0074] (1) Preparation of nano-golden flower particles with multi-level structure: the synthesis of nano-golden flowers is based on the regrowth method of nano-crystal seeds, and mainly includes the following steps:

[0075] First, the synthesis of gold nanoseed crystals: 100 ml, 2.5×10 -4 The mole / liter chloroauric acid aqueous solution was heated to 120°C, and 10 milliliters of 1% sodium citrate aqueous solution was added, and after stirring for 30 minutes, the solution turned wine red, and the heating was stopped and cooled to room temperature for subsequent use.

[0076] The second, the configuration of multi-level structure nano golden flower growth liquid: get 0.4 milliliter, 24 mmol / liter of chloroauric acid aqueous solution and add in 35% polydiallyl dimethyl ammonium chloride (PDDA) aqueous solution, room temperature Stir for 15 minutes. Then add 0.8 ml, 0.1 mol / L ascorbic acid aqueous solution, and stir for 30 seconds until the solution becomes colorless and transpa...

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Abstract

The invention discloses a multi-level structure nano gold flower, its preparation method and application. In one embodiment, the preparation method comprises: fully mixing chloroauric acid and polydiallyl dimethyl ammonium chloride in an aqueous phase system, and continuously stirring at room temperature until the formed mixed solution turns yellow , then add ascorbic acid to the obtained yellow mixed solution, and stir vigorously, then add nano-gold spheres, stir quickly, and then stand at room temperature. The preparation method is simple and easy to operate, has good controllability and high yield, and the surface of the obtained multi-level structure nano gold flower has nano-scale tip protrusions and a very large specific surface area. The nano-golden flower can be used as the gold-labeled antibody of the immunochromatographic test strip in the fields of food safety, environment, medical diagnosis, etc., to realize the simple and highly sensitive analysis and detection of the target object, for example, it can detect Escherichia coli with high sensitivity, The lower limit of detection concentration reached 103CFU / mL.

Description

technical field [0001] The invention relates to a nano-golden flower with a multi-level structure, its preparation method and application, such as the application in the preparation of a high-sensitivity detection kit based on an immune test strip, and belongs to the technical field of material science and analysis and detection. Background technique [0002] As an extremely important member of the nanoparticle system, gold nanoparticles also have unique physical and chemical properties, such as good biocompatibility, making gold nanoparticles widely used in biomarkers and biosensing. At present, the preparation methods of nano gold mainly include: liquid phase reduction method, photochemical method, gas phase evaporation method, seed crystal growth method, electrochemical reduction method, phase transfer method, hydrothermal method, microwave method, etc. However, most of these methods can only be used to synthesize spherical gold nanoparticles, and are not suitable for pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B22F1/00G01N33/569G01N33/558B82Y30/00B82Y40/00
Inventor 黄又举陈涛张磊
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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