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Bacillus for efficiently degrading soybean meal antigenic protein and method for fermenting soybean meal

A technology of antigenic protein and bacillus, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of insignificant degradation of soybean meal antigenic protein and low nutrient loss, so as to facilitate animal absorption and utilization, and acid-soluble The effect of increasing the protein content and increasing the content of acid-soluble protein

Active Publication Date: 2017-10-31
上海欧耐施生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The anaerobic fermentation feed has low nutrient loss and can provide a large amount of probiotics and metabolites, but the degradation of soybean meal antigen protein is not obvious by anaerobic fermentation

Method used

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  • Bacillus for efficiently degrading soybean meal antigenic protein and method for fermenting soybean meal
  • Bacillus for efficiently degrading soybean meal antigenic protein and method for fermenting soybean meal
  • Bacillus for efficiently degrading soybean meal antigenic protein and method for fermenting soybean meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: bacterial strain screening

[0042] 1. Separation 1g of traditional pickled vegetables in Jiangsu and Zhejiang regions, dissolved in 10ml sterile water, bathed in 75℃ water for 15min, then diluted with sterile water 10 times, and took 4 dilution gradients (10 -3 、10 -4 、10 -5 、10 -6 ) bacteria suspension on LB plates, at least 3 plates for each dilution, cultured at 37°C for about 24 hours to obtain a single colony.

[0043] 2. Preliminary screening Pick a single bacterial colony on the soybean meal antigen protein plate, incubate at 37°C for 24 hours, select the strain with a large hydrolysis zone, examine it under a microscope, and purify it by streaking to obtain the primary screening strain.

[0044] 3. Re-screening The strains were re-screened based on the degradation rate of soybean globinin and β-conglycinin based on the indicators. The specific process is as follows: put the strains obtained from the primary screening into LB liquid medium, unde...

Embodiment 2

[0047] Example 2: Characteristics of bacterial strains

[0048] 1. Morphological characteristics:

[0049] The strain ZJUFA-3 was streak cultured on LB medium at 37°C for 24 hours, and the growth morphology of the strain was observed. The results were as follows: figure 2 shown.

[0050] according to figure 2 As a result, the colony is milky yellow, nearly round, with a moist surface and translucent.

[0051] Pick a single colony therein and carry out Gram staining. After staining, it is purple, Gram-positive bacteria, oval, short rod-shaped, and spore-forming.

[0052] 2. Physiological and biochemical characteristics:

[0053] Pick a single colony among them, refer to the "Common Bacteria Identification Manual", and conduct physiological and biochemical characteristics detection. The detection items and results are shown in Table 2.

[0054]

[0055] 3. Genotype identification

[0056] The 16SrDNA sequence of ZJUFA-3 and the conserved gene gryB sequence were comp...

Embodiment 3

[0059] Embodiment 3: Fermentation effect comparison of several pretreatment processes of soybean meal

[0060] 3.1 Soybean Meal Pretreatment

[0061] Method 1. Add water and mix soybean meal (moisture content 11.13%), adjust the water content to 50%, do not sterilize, and obtain pretreated soybean meal 1;

[0062] Method 2. Add water and mix soybean meal (moisture content 11.13%), adjust the water content to 50%, and sterilize at 105°C for 15 minutes to obtain pretreated soybean meal 2;

[0063] Method 3. Soybean meal (moisture content 11.13%) was sterilized at 115°C for 15 minutes, then the water content was adjusted to 50wt%, and stirred evenly to obtain pretreated soybean meal 3.

[0064] 3.2 Fermentation of pretreated soybean meal

[0065] Take Bacillus subtilis ZJUFA-3, inoculate it in LB liquid medium, cultivate it at 37°C and 200 rpm for 12 hours, and obtain Bacillus subtilis ZJUFA-3 seed solution;

[0066] Add Bacillus subtilis ZJUFA-3 seed solution to the pretreate...

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Abstract

The invention discloses a bacillus capable of efficiently degrading soybean meal antigenic protein and a method for fermenting soybean meal. The bacillus belongs to Bacillus subtilis subsp.subtilis, is named ZJUFA‑3, and is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms. A method for fermenting soybean meal by using the Bacillus subtilis strain ZJUFA-3, 1) adding water to the soybean meal raw material until the water content is 40%-55% by mass; 2) adding OD600 value of 1.2 strain culture solution to the final concentration by mass percent 3%~8%, mix well, and ferment aerobically at 30~40℃ for 12~48 hours. The Bacillus subtilis ZJUFA‑3 provided by the invention can degrade most of the antigenic proteins within 24 hours, can also significantly increase the content of acid-soluble proteins, and significantly improve the quality of fermented soybean meal, and can greatly shorten the fermentation time and improve production efficiency when applied to production.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a bacillus for efficiently degrading anti-nutritional factors of soybean meal and a method for fermenting soybean meal. Background technique [0002] There are many anti-nutritional factors in soybean meal, such as antigenic protein, trypsin inhibitor, phytic acid, etc. The existence of these anti-nutritional factors affects the absorption of nutrients in feed by livestock and poultry, leading to the growth of harmful microorganisms in the intestinal tract, which in turn causes food source Diseases such as vomiting, diarrhea. Degrading anti-nutritional factors in feed through microbial fermentation and improving the absorption of nutrients in feed by animals has become an important way of "nutrition detoxification". [0003] my country's fermented feed is mainly based on solid-state fermentation, including aerobic fermentation and anaerobic fermentation. Aerobic fermentat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12C12R1/125
CPCC12N1/205C12R2001/125
Inventor 汪以真张煜石常友
Owner 上海欧耐施生物技术有限公司
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