Silkworm silk/collagen composite scaffold and preparation and application thereof

Abstract

The invention discloses a silkworm silk / collagen composite scaffold and preparation and application thereof. The preparation of the scaffold comprises: adding a silk fibroin solution into a mold and curing at -80 DEG C; drying and de-molding; then immersing in an ethanol water solution for 0.5h to 2h; storing for 0.5h to 2h under the conditions that the temperature is 35 DEG C to 75 DEG C and the humidity is 70 percent to 100 percent; airing to obtain a cross-linked silk fibroin scaffold; immersing the cross-linked silk fibroin scaffold into a collagen solution and vacuumizing; dropwise adding one layer of the collagen solution on the surface of the cross-linked silk fibroin scaffold and drying in vacuum; storing and crossly linking at 25 DEG C to 110 DEG C to obtain the silkworm silk / collagen composite scaffold. The silkworm silk / collagen composite scaffold disclosed by the invention has good mechanical properties and biocompatibility and overcomes the defects of a single-material scaffold; the silkworm silk / collagen composite scaffold can bear a meniscus and stabilize a knee joint at the early period of transplanting, and has the effects of conducting load, protecting joint cartilages and the like.

Description

(1) Technical field [0001] The invention relates to a composite bracket material for knee meniscus defect repair and a preparation method thereof, in particular to a preparation method of a silk / collagen composite bracket and its application in meniscus defect repair. (2) Background technology [0002] The meniscus is a fibrous cartilage tissue located between the femoral and tibial plateaus of the knee joint, which can transmit loads, absorb shocks, maintain the stability of the knee joint, and protect the cartilage. In sports activities and daily life, the meniscus is prone to acute injury (mostly in young people under 40 years old) and chronic strain (mostly in people over 65 years old) due to various stimuli such as impact, extrusion, grinding and pulling. elderly people). With the growth of age, the meniscus also gradually undergoes its own degeneration, which leads to a significant increase in the probability of its damage, up to about 60%. In the United States, ther...

AI Technical Summary

Problems solved by technology

In the meniscus tissue engineering technology, the degradable tissue engineering scaffold CMI (collagen meniscus implant) became the first commercialized product, but the follow-up clinical application research found that the initial mechanical properties of the pure collagen scaffold were insufficient, and the degradation in vivo was too fast, so it could not To achieve the long-term function of replacing the natural meniscus, so it cannot protect the articular cartilage

Some researchers have used degradable polymer synthetic materials to make meniscus tissue engineering scaffolds. Although their mechanical properties have been improved, their biocompatibility is poor, and the degradation products have certain toxicity.

Patent 200710192209.2, the preparation method of medical knee meniscus, provides a composite braided silk and collagen scaffold, but it cannot provide continuous mechanical strength as the collagen degrades

In short, the current research on meniscus tissue engineering scaffolds cannot bionically simulate the complete structure and function of the normal meniscus in vivo, and cannot provide appropriate mechanical strength, so it is difficult to effectively protect articular cartilage

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