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Beta-TCP/PGS composite scaffold and preparation method and application thereof

A composite stent and porous stent technology, which can be used in pharmaceutical formulations, medical preparations with non-active ingredients, medical science, etc., can solve the problems of limited improvement in β-TCP toughness and strength, poor toughness, and the influence of β-TCP on biological activity. , to achieve the effect of promoting vascularization, rapid repair, and promoting bone repair

Inactive Publication Date: 2015-05-06
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of the inherently poor toughness of these polymers, there is limited improvement in toughness and strength for β-TCP
At the same time, the content of too high polymer material will affect the biological activity of β-TCP

Method used

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  • Beta-TCP/PGS composite scaffold and preparation method and application thereof
  • Beta-TCP/PGS composite scaffold and preparation method and application thereof
  • Beta-TCP/PGS composite scaffold and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Synthesis and Purification of PGS Prepolymer

[0105] PGS prepolymers are synthesized by a two-step process.

[0106] Step 1: Under an argon atmosphere, react sebacic acid and glycerin at a molar ratio of 1:1 at 130°C for 24 hours;

[0107]The second step: the product of the first step was continued to react for 24 hours at 130° C. under vacuum conditions to obtain a PGS prepolymer.

[0108] Purification is then carried out. Firstly, the PGS prepolymer is dissolved in ethanol, the obtained emulsion is allowed to stand for a period of time, and the supernatant liquid is poured off. The obtained PGS prepolymer was placed in a dialysis bag and dialyzed to obtain a purified PGS prepolymer with a molecular weight of 12000 Da and a dispersion coefficient of 1.19.

[0109] Purification of PGS prepolymer 1 H-NMR such as figure 1 Shown in A. The proton peaks at 1.31, 1.62 and 2.35ppm mainly indicate the presence of sebacic acid units in the synthesized polymer chains, and t...

Embodiment 2

[0113] Synthesis of β-TCP Nanoparticles and Preparation of β-TCP Porous Scaffold

[0114] 0.4mol Ca(NO 3 ) 2 4H 2 O and 0.4mol(NH 4 ) 2 HPO 3 Dissolve in 200ml ultrapure water respectively, and stir rapidly for 30min to obtain Ca salt and P salt solutions. Add the Ca solution to the P solution drop by drop (1-2 drops / second), while maintaining constant stirring and adding the prepared ammonia water (2M) to adjust the pH value of the mixed solution to be stable at 7.0-7.5. After the dropwise addition was completed, the stirring was stopped, and the solution was allowed to stand for 2 hours to allow the solution to precipitate naturally, and the supernatant liquid was poured off. Then put the obtained precipitate into a centrifuge bottle, carry out centrifugation (6000r / min, 10min), wash with ultrapure water in the next step, ultrasonic (10min), continue to centrifuge, then repeat the water wash 2 times, and then wash it again with absolute ethanol After centrifugation, t...

Embodiment 3

[0121] Preparation of β-TCP / 10P-Unc Porous Scaffold

[0122] The prepared PGS prepolymer (PGS-1:1, prepared in Example 1) was formulated into a solution with a concentration of 10 mg / ml using absolute ethanol as a solvent. Place the β-TCP porous scaffold (0.2 g in weight) in a 24-well plate, add 2 ml of PGS solution to completely submerge the scaffold, and then place the porous plate in a fume hood for 3 to 5 hours to remove ethanol. Then the scaffold was freeze-dried at -50° C. under vacuum conditions for 24 hours to obtain the product β-TCP / 10P-Unc porous scaffold. The size of the pores is between 300-500 microns. The porosity is 90±2%. The maximum compressive strength is 0.02±0.01MPa, and the fracture deformation is 120±32%.

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Abstract

The invention discloses a Beta-TCP / PGS composite scaffold and a preparation method and application thereof. The Beta-TCP / PGS composite scaffold comprises PGS (poly glycerol sebacate) and a Beta-TCP (Tertiary Calcium Phosphate) porous scaffold. Particularly, the Beta-TCP / PGS composite scaffold is prepared by impregnating the Beta-TCP porous scaffold with a PGS prepolymer solution and then preforming thermal-crosslinking and drying. The invention further discloses a medicine composition and a tissue-engineered bone repair material, which comprise the composite bracket. Compared with a pure Beta-TCP porous scaffold, the Beta-TCP / PGS composite scaffold prepared by the invention shows excellent tenacity and compression strength, meanwhile has favorable biocompatibility and can be used as a bone repair material and a medicine carrier material.

Description

technical field [0001] The invention relates to a β-tricalcium phosphate / polymer material composite scaffold system for bone tissue repair and its preparation method and application, in particular to a β-tricalcium phosphate / polyglyceryl sebacate composite scaffold system and its preparation method and apply. Background technique [0002] β-tricalcium phosphate (β-TCP) is similar to the main inorganic components in human natural bone, has good biocompatibility and biodegradability and excellent osseointegration ability, and has high hardness and rigidity, which is convenient for acceleration Therefore, it has been widely used in bone tissue repair engineering, and it is also one of the most widely used bone repair materials so far. Among them, the β-TCP porous scaffold material, which is convenient for cells and ingrowth, has attracted extensive attention from researchers at home and abroad. However, the single β-tricalcium phosphate porous scaffold has problems such as hi...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/12A61L27/38A61L27/54A61L27/56A61K47/34
Inventor 刘昌胜杨凯张婧袁媛李玉林马一帆
Owner EAST CHINA UNIV OF SCI & TECH
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