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Production method of viral, fungal and biological insect prevention organic fertilizer

A technology of biological pest control and production method, applied in the direction of organic fertilizer, fertilizer made from biological waste, fertilization device, etc., to achieve the effect of increasing area yield, fertilizing soil fertility, and increasing organic matter

Inactive Publication Date: 2016-12-07
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for making virus and fungus biological insect-proof organic fertilizer, which can solve the problem of fertilizer treatment in the large-scale production of insect viruses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Mix wheat flour, soybean flour and yeast powder in a ratio of 4.5:4.5:1, autoclave for 15 minutes, and dissolve the agar with boiling water at the same time, the agar content is 1.5%; pour the sterilized powder into the agar-containing liquid, The ratio of solid to liquid is 1:6, add a small amount of sorbic acid and stir evenly, cut into small pieces after cooling, and feed insect larvae; place 10-13 newly hatched larvae on each piece of feed, and raise them in insect feeding trays ; Feed for 7 days at 26°C, spray 5×10 on the feed 6 PIB / mL virus suspension infects larvae, feeds them at 28°C for 5 days and starts to collect virus-dead larvae, and collects them after 8 days; then collect the remaining feed stained with insect virus, dry it at 40-55°C, and reduce the water content to 5-5°C. 10%, then pulverized, the virus content was counted, and a small amount of virus mother solution was added to make the virus content in the feed waste reach 800 million virus protein i...

Embodiment 2

[0032] Mix wheat flour, soybean flour, corn flour and yeast powder in a ratio of 3:3:3:1, autoclave for 15 minutes, and dissolve agar with boiling water at the same time, the agar content is 1.3%; pour the sterilized powder into Agar-containing liquid, the ratio of solid to liquid is 1:7, add a small amount of sorbic acid and stir evenly, cut into small pieces after cooling, and feed insect larvae; place 10-15 newly hatched larvae on each piece of feed. Rearing in insect trays. Feed for 6 days at 26°C, spray 1×10 on the feed 6 PIB / mL of broad-spectrum cabbage spodoptera nuclear polyhedrosis virus suspension infects larvae, feeds at 28°C for 5 days to collect virus-killed larvae, and collects them after 8 days. After the virus-killed insect corpses are collected, collect the remaining feed that is stained with insect virus in the insect culture equipment, dry it at 40-55°C to reduce the water content to 5-10%, then crush it, count the virus content in it, and add A small amou...

Embodiment 3

[0035]Virus fungus bio-insect control organic fertilizer is composed of the following components, by weight ratio, 70% of leftover feed containing 500 million PIB / gram of broad-spectrum insect virus, 10% of humic acid, 10% of rice bran, 10% of rapeseed cake, 20% Billion spores / g Paecilomyces lilacinus Paecilomyces lilacinus PL461 powder 5%; after all components are mixed evenly, check the package.

[0036] The virus and fungus bio-insect-proof organic fertilizer is applied in Shandong peanut fields, 5 kg per mu, and applied continuously for two years, which can effectively control underground harmful insects, root-knot nematodes and other harmful insects, and increase the yield by an average of 20-30% compared with the control field.

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Abstract

The invention discloses a production method of a viral, fungal and biological insect prevention organic fertilizer, and relates to the technical field of biologic treatment of insect breeding residues in insect virus production. The production method comprises the following steps: 1, preparing an insect feed; 2, carrying out group breeding on early-stage health larvae; 3, carrying out virus inoculation and virus propagation; 4, collecting residual feed with insect viruses; and 5, preparing the viral, fungal and biological insect prevention organic fertilizer according to a formula. The residual feed contains wide-spectrum insect viruses, and a small amount of nematicidal fungi is added, so the prepared viral, fungal and biological insect prevention organic fertilizer has good application prospect and good market development values; the viral, fungal and biological organic fertilizer effectively prevent and control underground pests, increases organic matters, improves the granular structure of soil, fertilizes the soil fertility, improves the quality of crops, improves the output of per unit of area, and recovers ecologic balance; and the organic fertilizer can effectively control harmful insects and root-knot nematodes according to a dosage of several to 10 kilograms / 666.6 m<2>, and effectively improves the output and the output value per unit of area.

Description

technical field [0001] The invention relates to the technical field of biological treatment of insect feeding residues in the production of insect viruses, in particular to a method for preparing a virus fungus bio-insect-proof organic fertilizer. Background technique [0002] Insect baculovirus insecticide is a safe biological pesticide recommended by the World Food and Agriculture Organization. It has good insecticidal effect, is safe for humans and higher animals, does not pollute the environment, does not harm natural enemies, and is not easy to cause pests to develop resistance. Vigorously develop ecological biological pesticides. [0003] Traditionally, one type of baculovirus can only control one type of pest, which greatly limits the industrialization and application of insect viruses. The development of broad-spectrum insect baculovirus insecticides has become one of the directions for the development of the virus biopesticide industry. Cabbage armyworm nuclear pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/02C05G3/60
CPCC05F5/002C05G3/60C05F11/00C05F11/02
Inventor 张忠信胡秀筠吴柳柳类承凤
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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