A new strain of Bacillus licheniformis and its application

A technology of Bacillus licheniformis and new strains, applied in application, bacteria, chemicals for biological control, etc., can solve problems such as the destruction of soil beneficial microbial flora, affecting the quality of agricultural products, and insufficient supply of organic fertilizers, and reaching the shelf life The effect of increasing soil fertility, solving the increasingly low soil fertility, and improving soil fertility performance

Active Publication Date: 2019-04-19
廊坊市精耕天下农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term application of chemical fertilizers, the soil beneficial microbial flora is damaged, the supply of organic fertilizer is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical properties and soil microbial flora. The current status of soil resources in my country urgently needs to solve the problem of soil being gradually destroyed through artificial measures to ensure the level of agricultural planting and the quality of crop products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Mycological characteristics of Bacillus licheniformis CLIMAX-VBL

[0028] Strain CLIMAX-VBL is isolated, purified and rejuvenated from the soil of crop roots. Its morphological characteristics are as follows: the cells are rod-shaped, Gram-positive, and the size is (0.6-0.8μm)×(2.0-3.5μm). The spores are terminal or partial, cylindrical, and the cysts are not enlarged. On broth medium, colonies are round, moist, with neat edges and red pigment.

[0029] Physiological and biochemical characteristics are as follows: contact enzyme, oxidase, amylase test was positive; anaerobic growth; propionate available; lecithinase test was negative.

Embodiment 2

[0030] Example 2 Identification of Bacillus licheniformis CLIMAX-VBL

[0031] Through the determination of the 16S rDNA sequence of the strain CLIMAX-VBL, the sequencing results were compared in GenBank, and it was found that the sequence homology with other published 16S rDNA of Bacillus licheniformis was relatively high.

[0032]Through the above-mentioned morphological characteristics, physiological ecological characteristics and 16S rDNA sequence analysis, it can be confirmed that a new strain of Bacillus licheniformis was isolated.

Embodiment 3

[0033] Example 3 Preparation of Bacillus licheniformis CLIMAX-VBL microbial inoculum (liquid fermentation mode)

[0034] The strain CLIMAX-VBL stored in the freeze-dried tube was inserted into the solid slant of the broth medium, and cultivated at 32 °C for 24 hours; 7 days; then pick the colony and insert it into the sterilized liquid medium, and cultivate it for 3 days under the conditions of 32 ° C and 150 r / min to obtain the fermentation broth of the strain CLIMAX-VBL, and the fermentation broth is directly used as a liquid bacterial agent, Among them, the viable count of Bacillus licheniformis CLIMAX-VBL is more than 1 billion / g.

[0035] The liquid culture medium contains the following components by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate and 1000 parts of water.

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PUM

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Abstract

The invention provides a new strain of Bacillus Licheniformis CLIMAX‑VBL, and its preservation number is CGMCC No.12499. The present invention also provides bacterial agents containing bacillus licheniformis CLIMAX‑VBL and applications thereof. The fungus can significantly improve crop yield and stress resistance, and significantly reduce crop diseases. The strain CLIMAX‑VBL has been industrialized and has broad application prospects. It can be used to configure a variety of fertilizer products, and can be used for seedbeds, root irrigation, and fertilizer (making bio-organic fertilizer) and other purposes.

Description

technical field [0001] The invention relates to the field of agricultural microbial preparations and soil fertilizers, in particular to a new strain of Bacillus licheniformis and its application. Background technique [0002] White agriculture refers to the industrialized new agriculture of the industrialization of microbial resources, usually referring to the application of microbial fermentation engineering in agriculture. Agricultural production requires soil to provide nutrients and living environment for crop growth. Over the years, the application of chemical fertilizers has played a key role in increasing agricultural income. However, due to the long-term application of chemical fertilizers, the beneficial microbial flora in the soil is destroyed, the supply of organic fertilizers is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical prope...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N59/16A01N59/06A01P21/00C05G3/00C05G3/04C12R1/10C05G3/80
CPCA01N59/06A01N59/16A01N63/00C05F11/02C05G3/00C12N1/20C05G3/80C12N1/205C12R2001/10A01N2300/00C05F11/08
Inventor 刘澎
Owner 廊坊市精耕天下农业科技有限公司
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