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Method for determining contents of tocopherols and tocotrienols in edible vegetable oil by using gas chromatography-positive chemical ionization-mass spectrometry technology

A kind of technology of tocotrienol, edible vegetable oil

Active Publication Date: 2016-12-07
ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, Fourier transform infrared spectroscopy is mainly used in the determination of α-tocopherol in vegetable oils with a single component and less interference, and its application range is limited; electrochemical analysis needs to be combined with chemometrics to better realize the detection of α-tocopherol in vegetable oil samples. Simultaneous determination of multiple vitamin E isomers is complex and difficult to understand and master; chromatography, especially liquid chromatography, is currently the most commonly used analytical technique for vitamin E, but β-tocopherol and γ-tocopherol have similar structures , it is difficult to separate

Method used

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  • Method for determining contents of tocopherols and tocotrienols in edible vegetable oil by using gas chromatography-positive chemical ionization-mass spectrometry technology
  • Method for determining contents of tocopherols and tocotrienols in edible vegetable oil by using gas chromatography-positive chemical ionization-mass spectrometry technology
  • Method for determining contents of tocopherols and tocotrienols in edible vegetable oil by using gas chromatography-positive chemical ionization-mass spectrometry technology

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Embodiment 1

[0032] Example 1 Method for Determination of Tocopherol and Tocotrienol Content in Edible Vegetable Oil

[0033] Determination of tocopherol and tocotrienol content in virgin olive oil by gas chromatography-positive chemical source-mass spectrometry, the specific detection objects are α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol , α-tocotrienol, β-tocotrienol, γ-tocotrienol and δ-tocotrienol.

[0034] The specific method is as follows:

[0035] (1) extract

[0036] Accurately weigh 0.1 g of virgin olive oil sample into a 50 mL centrifuge tube. Add 10 mL of chromatographically pure methanol, thoroughly vortex and mix for 1 min, ultrasonically dissolve for 30 min, and take 1 mL of the clear liquid to obtain the extract.

[0037] (2) Preparation of standard working solution

[0038] Accurately weigh α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol and δ-tocotriene Dissolve 10.0 mg of phenol standard into a 10 mL br...

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Abstract

The invention provides a method for determining the contents of tocopherols and tocotrienols in edible vegetable oil by using gas chromatography-positive chemical ionization-mass spectrometry technology, relating to the field of food detection. The method comprises the following steps: detecting a standard working solution containing tocopherols and tocotrienols by adopting the gas chromatography-positive chemical ionization-mass spectrometry technology, obtaining the retention time and the abundance ratio of monitoring ions of each substance and carrying out linear regression analysis on the concentration of each substance according to the chromatographic peak areas of quantitative ions of each substance in the standard working solution, thus obtaining the working curve of each substance; extracting tocopherols and tocotrienols in edible vegetable oil, thus obtaining an extracting solution; detecting the extracting solution by adopting the gas chromatography-positive chemical ionization-mass spectrometry technology, judging whether the edible oil contains tocopherols and tocotrienols according to the retention time and the abundance ratio of monitoring ions and computing the concentration of each substance in the edible oil according to the working curve of each substance. The method is simple and fast and has high selectivity, sensitivity and accuracy.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for measuring the content of tocopherol and tocotrienol in edible vegetable oil by gas chromatography-positive chemical source-mass spectrometry. Background technique [0002] Vitamin E, also known as tocopherol, anti-sterility vitamin, etc., is a general term for a class of derivatives with d-α-tocopherol activity. Vitamin E is a powerful and effective antioxidant that can prevent oxidation by scavenging oxygen free radicals or interfering with oxide chain reactions, thereby preventing lipid peroxidation in cell membranes and a series of damages caused by it, and can improve the body's immunity , anti-aging, neuroprotection, inhibition of cholesterol synthesis and tumor cell growth, prevention of arteriosclerosis and cardiovascular and cerebrovascular diseases and other physiological functions, it also has a certain impact on reproductive function and cell metabolism. [...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/025
Inventor 沈伟健张睿魏雪缘蔡理胜刘芸陆慧媛沈崇钰吴斌赵增运柳菡
Owner ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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