Microfluidic chip shear flow based microcapsule preparation method

A technology of microfluidic chips and microcapsules, which is applied in the direction of fluid controllers, chemical instruments and methods, and laboratory containers, etc., can solve the problems of complex preparation process, destruction of surface activity, uneven dispersion, etc. Simple, good biological activity, evenly dispersed effect

Inactive Publication Date: 2016-12-21
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned existing microcapsule preparation methods having the disadvantages of destroying surface activity, uneven dispersion, complex preparation process, high equipment requirements, and high cost, the purpose of the present invention is to provide a simple, uniformly dispersed, and size-controllable microcapsule Capsule preparation method

Method used

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  • Microfluidic chip shear flow based microcapsule preparation method
  • Microfluidic chip shear flow based microcapsule preparation method
  • Microfluidic chip shear flow based microcapsule preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of the microfluidic chip hydrogel positive mold 12, the specific steps are as follows:

[0032] Step 1 Cleaning of the cover glass substrate 10: Put the cover glass substrate 10 into a beaker, pour in an appropriate amount of concentrated sulfuric acid, heat it on a heating furnace for 10 minutes, then take it out and cool it for 10 minutes, and rinse it with deionized water Concentrated sulfuric acid remaining on the surface of the coverslip substrate 10. Dry it with a cotton ball and put it into a beaker containing acetone, then put the beaker into an ultrasonic cleaner and vibrate for 10 minutes, take it out, rinse it with deionized water, and blow dry its surface moisture with nitrogen, as figure 2 as shown in a.

[0033] Step 2 Preparation of the sodium alginate layer 11: configure a 2mol / L sodium alginate solution, place the cover glass substrate 10 on the rotating table of a spin coater, set the spin coating speed to 800r / min, and coat the sodiu...

Embodiment 2

[0037] The preparation of the microfluidic chip hydrogel male mold 12, the specific steps are as follows:

[0038] Steps 1 and 2 are the same as steps 1 and 2 described in Embodiment 1.

[0039] Step 3: Preparation of the internal bi-conical glass micro-nozzle 5: The internal bi-conical glass micro-nozzle with an inner diameter of 100 μm was prepared by using the method for preparing the internal bi-conical glass micro-nozzle. The calcium chloride solution is loaded into the built-in biconical glass micro-nozzle 5.

[0040] Step 4 is the same as Step 4 described in Embodiment 1. The obtained microfluidic chip hydrogel male mold 12 has a height of 90 μm and a width of 250 μm, such as figure 2 c shown. .

Embodiment 3

[0042] The preparation of the microfluidic chip hydrogel male mold 12, the specific steps are as follows:

[0043] Steps 1, 2, and 3 are the same as steps 1, 2, and 3 described in Embodiment 1.

[0044] Step 4 Preparation of the microfluidic chip hydrogel male mold 12: set the driving voltage amplitude of the piezoelectric actuator 3 to 50V, the driving frequency to 5Hz, and set the motion parameters of the two-dimensional table 9 so that the droplet overlap rate is 50%, the motion pattern of stage 9 selects the T-cut pattern of the microfluidic chip. Drive the built-in double-cone glass micro-nozzle 5, and spray the calcium chloride solution onto the cleaned sodium alginate layer 11 to obtain a microfluidic chip hydrogel male mold 12, and the number of repetitions is set is 2, the height of the hydrogel positive mold can be increased, and the obtained microfluidic chip hydrogel positive mold 12 has a height of 120 μm and a width of 150 μm, such as figure 2 c, 2d shown.

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Abstract

The invention discloses a microfluidic chip shear flow based microcapsule preparation method. The method includes the steps of: adopting droplet micro-spray technique to prepare a micro-fluidic chip hydrogel male mould on a glass substrate spin-coated with a sodium alginate coating; then preparing a PDMS micro-fluidic chip negative mould by molding; and bonding the micro-fluidic chip negative mould and the glass substrate together by means of reversible bonding, thus obtaining a micro-fluidic chip; letting a kerosene solution and water of a microcapsule pass through liquid inlets of the micro-fluidic chip respectively through injection pumps, thus obtaining the needed microcapsule at a liquid outlet. According to the invention, the microfluidic chip preparation process is simple, the cost is low, preparation of the hydrogel male mould has no need for specific template, the hydrogel male mould with arbitrary pattern and controllable height can be prepared, and the microfluidic chip has smooth flow channel and large depth-to-width ratio. The prepared microcapsule has the advantages of good consistency and uniform dispersion, uniform and controllable particle size, and good biological activity.

Description

technical field [0001] The invention relates to the field of preparation of microcapsules, in particular to a method for preparing microcapsules based on microfluidic chip shear flow. Background technique [0002] Due to its excellent immune isolation performance and controlled release performance, microcapsules are expected to become an important tool for the practical and industrialization of upstream research results in bioengineering, and promote the clinical application of tissue cell or recombinant cell transplantation technology, disease treatment, and protein and polypeptide biochemical drugs. application. Since the 1990s, the medical community has begun to try to use microcapsules as an immune isolation and delivery tool for genetically recombined cells to use the metabolites of recombined cells to regulate the body's physiological functions and treat related diseases. [0003] According to the formation or dispersion method of core material droplets, the commonly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/50273B01L2200/12B01L2400/0415
Inventor 杨利军朱丽
Owner NANJING UNIV OF SCI & TECH
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