A method for fermenting and producing Bacillus subtilis with chondroitin sulfate production industrial wastewater as raw material

A technology of Bacillus subtilis and chondroitin sulfate, which is applied in the field of microbial fermentation, and achieves the effects of low equipment requirements, reduced burden, and simple process

Inactive Publication Date: 2019-04-05
香河县龙津生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is no literature that discloses the technical content of this aspect.

Method used

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  • A method for fermenting and producing Bacillus subtilis with chondroitin sulfate production industrial wastewater as raw material
  • A method for fermenting and producing Bacillus subtilis with chondroitin sulfate production industrial wastewater as raw material
  • A method for fermenting and producing Bacillus subtilis with chondroitin sulfate production industrial wastewater as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Use animal cartilage to extract 10L of industrial wastewater produced by the process of extracting chondroitin sulfate, add 50g of molasses according to the mass ratio of 0.5%, mix and dissolve, use NaOH or HCl to fine-tune the pH=6.5-7.0, and extinguish at high temperature at 121°C Bacteria 20min, cooling, according to the final concentration of 5 × 10 5 Inject appropriate amount of Bacillus subtilis into each / mL medium, and the specific parameters of the 10L liquid fermentation tank are as follows: temperature control 37°C, tank pressure 0.05mpa, 0-6h speed 150rpm, ventilation volume 1:0.5; 6-24h speed 250rpm, ventilation volume 1:1.2; after 24 hours, the rotation speed is 150rpm, and the ventilation rate is 1:0.5. After 36 hours of fermentation, the number of Bacillus subtilis detected by the method of GB / T 26428-2010 "Detection of Bacillus subtilis in microbial preparations for feed" > 6.0×10 10 individual / mL. The fermented liquid is concentrated 10 to 2...

Embodiment 2

[0026] Example 2: Use animal cartilage to extract 10L of industrial wastewater produced by the process of extracting chondroitin sulfate, add 120g of molasses according to the mass ratio of 2.5%, mix and dissolve, use NaOH or HCl to fine-tune the pH=6.5-7.0, and extinguish at high temperature at 121°C Bacteria for 20min, cooled, according to the final concentration of 1 × 10 6 Inject appropriate amount of Bacillus subtilis into each / mL medium, and the specific parameters of the 10L liquid fermentation tank are as follows: temperature control 37°C, tank pressure 0.05mpa, 0-6h speed 150rpm, ventilation volume 1:0.5; 6-24h speed 250rpm, ventilation volume 1:1.2; after 24 hours, the rotation speed is 150rpm, and the ventilation rate is 1:0.5. After 48 hours of fermentation, the number of Bacillus subtilis detected by the method of GB / T 26428-2010 "Detection of Bacillus subtilis in microbial preparations for feed" > 5.0×10 11individual / mL. The fermented liquid is concentrated 10 t...

Embodiment 3

[0027] Example 3: 10L of industrial wastewater produced by the process of extracting chondroitin sulfate from animal cartilage was added to 200g of molasses according to a mass ratio of 5.0%, mixed and dissolved, then finely adjusted to pH=6.5-7.0 with NaOH or HCl, and extinguished under high temperature at 121°C Bacteria 20min, cooling, according to the final concentration of 5 × 10 6 Inject appropriate amount of Bacillus subtilis into each / mL medium, and the specific parameters of the 10L liquid fermentation tank are as follows: temperature control 37°C, tank pressure 0.05mpa, 0-6h speed 150rpm, ventilation volume 1:0.5; 6-24h speed 250rpm, ventilation volume 1:1.2; after 24 hours, the rotation speed is 150rpm, and the ventilation rate is 1:0.5. After 42 hours of fermentation, the number of Bacillus subtilis detected by the method of GB / T 26428-2010 "Detection of Bacillus subtilis in microbial preparations for feed" > 3.0×10 11 individual / mL. The fermented liquid is concent...

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Abstract

The invention provides a method for producing Bacillus subtilis by fermenting chondroitin sulfate production industrial wastewater. The method comprises the following steps: by using industrial wastewater generated by a chondroitin sulfate extraction technique from animal cartilage as a substrate and using mixed molasses as a culture medium, carrying out high-temperature sterilization, inoculating Bacillus subtilis, carrying out liquid fermentation, and concentrating the fermentation liquid to obtain a Bacillus subtilis concentrated solution. The method solves the problem of pollution of industrial wastewater generated by chondroitin sulfate production, effectively utilizes the waste industrial wastewater to produce the high-added-value Bacillus subtilis concentrated solution, and achieves the goal of changing wastes into valuable substances. The whole technique has the advantages of simple process, mild technical control conditions, lower facility requests and low investment, and is convenient for industrialized large-scale production application. The Bacillus subtilis concentrated solution obtained by the method has high living bacteria count.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for fermenting and producing Bacillus subtilis by using chondroitin sulfate production industrial wastewater as a raw material. Background technique [0002] Chondroitin sulfate exists in the form of proteoglycan in cartilage tissue, and the polysaccharide chain of chondroitin sulfate in proteoglycan is connected with the core protein by covalent bond (glycopeptide bond). The effective method destroys the covalent combination of polysaccharide chain and protein, so that the polysaccharide chain and protein can be effectively separated, and the polysaccharide chondroitin sulfate can be extracted efficiently. At present, domestic manufacturers often use the method of proteolysis of protein + isoelectric point precipitation of protein for protein dissociation. During the production process, a large amount of nitrogen-containing organic wastewater ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/125
CPCC12N1/20
Inventor 姜龙祥石苑婷吕伟杨
Owner 香河县龙津生物工程有限责任公司
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