Bladder membrane biological scaffold, preparation method and purpose thereof

A bio-scaffold, bladder membrane technology, applied in medical science, prosthesis, etc., can solve problems such as malignant transformation, infection, abnormal metabolism, etc., and achieve the effects of good biodegradability, reduction of rejection reaction, and good mechanical strength

Inactive Publication Date: 2016-12-28
BEIJING LANGJIAYI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the biocompatibility problems of the above-mentioned materials or the problems of metabolic abnormalities, infection, urinary calculi, contractures and even malignant transformati

Method used

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  • Bladder membrane biological scaffold, preparation method and purpose thereof
  • Bladder membrane biological scaffold, preparation method and purpose thereof
  • Bladder membrane biological scaffold, preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0043] The fresh bladders of cattle, pigs, and sheep that were killed within half an hour were taken, and quickly stored in PBS buffer (pH 7.4) in an ice box. Trim and remove excess tissues such as muscle and fat around the bladder. Cut it from the bottom of the bladder neck, and then cut the bladder in the middle to the top of the bladder. The whole bladder is spread out in a piece. Set aside after rinsing three times with PBS.

[0044] After that, follow the steps below to prepare the bladder membrane bio-scaffold:

[0045] (1) Immerse the bladder in Tris (pH 8.0, Amresco, code: 0497) buffer, the buffer contains the protease inhibitor PMSF (sigma catalog number 78830) (concentration 0.35ml / L), continue to soak and stir at room temperature for 24 hours , Change the buffer solution every 8 hours;

[0046] (2) Put the bladder in PBS (pH 7.4, Nissui, article number ZZR05913), and rinse on a shaker at 15°C at 60~80rpm for 1 hour;

[0047] (3) Soak in 2N NaOH aqueous solution for 30 min...

preparation example 2

[0066] Using a bovine bladder, the bladder membrane was prepared as in Preparation Example 1, except that step (5) and step (7) were exchanged in order and step (10) and step (12) were exchanged in order. Sample 4 is obtained.

[0067] Preparation comparative example

[0068] Using a bovine bladder, the bladder membrane was prepared as in Preparation Example 1, except that steps (3) and (14) were not performed. A comparative sample 1 was obtained.

Embodiment 1

[0069] Example 1 :Physical and chemical properties of bladder membrane

[0070] The appearance and physical and chemical properties of samples 1 to 4 and comparative sample 1 are similar, but products 1 to 4 are more thorough in killing bacteria and viruses and have less rejection reaction. Among them, the appearance of sample 1 is as figure 1 Shown.

[0071] (1) DNA content: According to the method specified in Appendix IX B of the Third Part of the Pharmacopoeia of the People's Republic of China.

[0072] The results show that the DNA content of samples 1 to 4 and comparative sample 1 is less than 0.5 wt%.

[0073] (2) Fat content: according to GB / T 5009.3-2010, the first method.

[0074] The results show that the fat content of samples 1 to 4 and comparative sample 1 is less than 0.5 wt%.

[0075] (3) Tensile strength: use the QJ212 tensile strength testing machine manufactured by Shanghai Qing Technology to measure.

[0076] The results show that the tensile strength of samples 1 t...

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Abstract

The invention provides a bladder membrane biological scaffold preparation method. The method comprises the following steps: 1) using NaOH for processing bladder tissues; 2) using TritonX-100 and SDS for processing the bladder tissues with any sequence, repeating the step; 3) using NaOH for processing the bladder tissues; and 4) drying the product at low temperature. In the method, a protease inhibitor can be used for processing the bladder tissues before the step 1), and nuclease can be used for processing the bladder tissues after processing by TritonX-100 and SDS in the step 2). The prepared bladder membrane biological scaffold has good biocompatibility and mechanical strength, and provides a tissues regenerating framework, and has the advantages that bacteria and virus can be thoroughly killed, the rejection is little, and the bladder membrane biological scaffold can be used for bladder restoration.

Description

Technical field [0001] The invention relates to a bladder membrane biological scaffold capable of guiding tissue regeneration and a preparation method and application thereof. Background technique [0002] As an important organ of the urinary system, the bladder is often damaged or defective due to some congenital causes or inflammation, infection, tumor, trauma, etc., which can cause the bladder to become smaller and damaged, which ultimately requires bladder enlargement And repair replacement. Therefore, it is necessary to use certain substitute materials for repair. There are three main sources of traditional substitute materials, organic synthetic materials, natural substitute materials from non-urinary system sources, and the patient's own urinary system tissues. However, due to the biocompatibility problems of the above-mentioned materials or the existence of metabolic abnormalities, infections, urinary calculi, contractures and even malignant changes, or due to limited t...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 魏桂清
Owner BEIJING LANGJIAYI BIOTECH CO LTD
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