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Method for increasing yield of camellia seed oil extracted with aqueous enzymatic method

A technology of camellia seed oil and water enzymatic method, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of increased production cost, chemical organic reagent residue, quality decline, etc., achieve low energy consumption, increase yield, and simple operation Effect

Pending Publication Date: 2017-01-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN102154059A introduces the use of alcohol method for demulsification. The invention has simple operation and high demulsification rate, but there will be chemical organic reagent residues in food, which will affect food safety and increase production costs.
CN104440174A discloses a microwave treatment demulsification method. Although no organic reagent is used, the microwave treatment reduces the quality of oil (acid value and peroxide value all increase), and the demulsification effect is not good, which affects the popularization and application of the method

Method used

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  • Method for increasing yield of camellia seed oil extracted with aqueous enzymatic method
  • Method for increasing yield of camellia seed oil extracted with aqueous enzymatic method
  • Method for increasing yield of camellia seed oil extracted with aqueous enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2-4

[0029] Example 2-4: Effect of Calcium Compound Type on Oil Extraction Rate and Emulsion to Total Sample Ratio

[0030] Take 5 grams of tea seed powder (40 mesh) in a 100ml beaker, add distilled water at a material-to-liquid ratio of 1:8, inactivate the enzyme in a 90°C water bath for 10 minutes, adjust the pH to 6.0 with 0.1mol / L NaOH solution, and add 0.1% ( w / v, g / mL) cellulase, hydrolyze at 50°C for 1 h, then adjust the pH to 9.0 with 2mol / L NaOH solution, add n Ca2+ :n 皂素 = 1:1 calcium-containing compound (types of calcium-containing compound include calcium chloride, calcium carbonate or calcium oxide), and 0.1% (v / v, mL / mL) Alcalase 2.4L alkaline protease, enzymolysis at 60°C for 4h. After enzymatic hydrolysis, inactivate the enzyme in a water bath at 90°C for 10 minutes; cool to room temperature, centrifuge the sample solution (8000rpm, 30min, 4°C), collect the clear oil in the upper layer and the emulsion in the middle layer, and calculate the ratio of clear oil and e...

Embodiment 5-8

[0034] Examples 5-8: Ca 2+ Effect of Addition Amount on Oil Extraction Rate and Ratio of Emulsion to Total Sample

[0035] Take 5 grams of tea seed powder (40 mesh) in a 100ml beaker, add distilled water at a material-to-liquid ratio of 1:8, inactivate the enzyme in a 90°C water bath for 10 minutes, adjust the pH to 6.0 with 0.1mol / L NaOH solution, and add 0.1% ( w / v, g / mL) cellulase, hydrolyze at 50℃ for 1h, then adjust the pH to 9.0 with 2mol / L NaOH solution, add n Ca2+ :n 皂素=1:3; 1:2; 2.5:1; 5:1 0.1mol / L calcium chloride, and 0.1% (v / v, mL / mL) Alcalase 2.4L alkaline protease, 60°C for 4h, After enzymatic hydrolysis, inactivate the enzyme in a water bath at 90°C for 10 minutes; cool to room temperature, centrifuge the sample solution (8000rpm, 30min, 4°C), collect the clear oil in the upper layer and the emulsion in the middle layer, and calculate the ratio of clear oil and emulsion to the total sample. The results are listed in Table 2.

[0036] Table 2 Ca 2+ Effect of...

Embodiment 9-12

[0039] Examples 9-12: Ca 2+ Effect of reaction time with saponin on oil extraction rate and ratio of emulsified product to total sample

[0040] Take 5 grams of tea seed powder (40 mesh) in a 100ml beaker, add distilled water at a material-to-liquid ratio of 1:8, inactivate the enzyme in a 90°C water bath for 10 minutes, adjust the pH to 6.0 with 0.1mol / L NaOH solution, and add 0.1% ( w / v, g / mL) cellulase, hydrolyze at 50°C for 1 h, then adjust the pH to 9.0 with 2mol / L NaOH solution, add 0.1% (v / v, mL / mL) Alcalase 2.4L alkaline protease, 60 ℃ enzymatic hydrolysis for 4h to extract, add n Ca2+ :n 皂素 =1:2 0.1mol / L calcium chloride (calculated as Ca 2+ The reaction time with saponin is 5h, 4h, 3h, 2h, 1h). After enzymatic hydrolysis, inactivate the enzyme in a water bath at 90°C for 10 minutes; cool to room temperature, centrifuge the sample solution (8000rpm, 30min, 4°C), collect the clear oil in the upper layer and the emulsion in the middle layer, and calculate the ratio ...

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Abstract

The invention provides a method for increasing the yield of camellia seed oil extracted with an aqueous enzymatic method. The method comprises steps as follows: camellia seed powder and water are mixed in the material-liquid mass ratio being 1:(1-10), the temperature is increased to 80-90 DEG C for enzyme deactivation for 5-10 min, the pH value is adjusted to 4.5-6.0, and a mixed solution is obtained; a calcium-containing compound is added to the mixed solution, and oil is extraction with the aqueous enzymatic method. According to the method, before an emulsion is formed, according to the characteristic that a triterpene glycoside substance (saponin) aqueous solution can react with calcium salt to produce precipitate, the saponin is precipitated, and the emulsion phenomenon caused by reaction of oil with the saponin is reduced, so that the yield of free oil is increased. The method has the advantages of being low in energy consumption, pollution-free, green, healthy and simple to operate.

Description

[0001] (1) Technical field [0002] The invention relates to a method for improving the yield of camellia seed oil extracted by aqueous enzymatic method, in particular, in the process of extracting camellia seed oil by aqueous enzymatic method, by using calcium-containing compounds to precipitate tea saponin, reducing the formation of emulsion, thereby improving The method of oil removal rate. [0003] (2) Background technology [0004] Camellia (scientific name: Camellia japonica L.) is a unique woody oil tree species in my country and one of the four largest woody oil tree species in the world. Camellia seed, as a by-product of camellia, is rich in active substances such as oil (40-50%), tea saponin (10%-20%), protein (10%), and the camellia seed oil made by it is rich in Unsaturated fatty acids, amino acids necessary for the human body, and trace elements such as zinc and selenium are high-grade edible oils that are loved by consumers and have broad market development poten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/04
CPCC11B1/025C11B1/04
Inventor 孟祥河葛杭丽潘丹杰叶沁
Owner ZHEJIANG UNIV OF TECH
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