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Extraction method of sulforaphane

A technology of sulforaphane and extraction method, applied in the directions of organic chemistry, fermentation, etc., can solve the problems of low conversion rate of sulforaphane, difficult industrialized production, few extraction methods of sulforaphane, etc., and achieves easy industrialized production and product quality. Stable and high extraction rate

Inactive Publication Date: 2017-01-25
徐金蝶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic reports on isothiocyanates such as sulforaphane are mainly about analysis and detection. There are few extraction methods for sulforaphane, and most of the prepared sulforaphane has a low conversion rate, and they are limited to the preparation of a small amount of experimental scale. products, it is difficult to carry out large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] S10, taking fresh broccoli seeds and dehydrating at 75°C for 2 hours, then drying at 65°C until the water content is lower than 8%, and then crushing to 45 mesh with a pulverizer to obtain broccoli seed powder. Take 15 g of broccoli seed powder as a raw material, add 300 mL of n-hexane to degrease, mix with a high-speed homogenizer at 40°C for 50 minutes, and filter to obtain a filter residue for later use;

[0030] S20, take 12 g of the filter residue, add 25 mL of 0.05 mol / mL phosphate buffer to adjust the pH to 5.5, add 36 mL of crude glucuronidase, stir well, and place it at 45 ° C for 1 hour for enzymatic hydrolysis; then add the mass concentration of 95% 500mL of ethanol was extracted for 45min by ultrasonic oscillation with a vibration frequency of 40kHz and a power of 200w, and then passed through a 30-mesh sieve after standing for 30min to remove the residue of the extract to obtain the filtrate; ) to obtain the extract after suction filtration to remove impuri...

Embodiment 2

[0034] S10, take fresh broccoli seeds and dehydrate them at 80° C. for 1 hour, then dry them at 60° C. until the moisture content is lower than 8%, and then crush them to 30 mesh with a pulverizer to obtain broccoli seed powder. Take 20 g of broccoli seed powder as a raw material, add 400 mL of n-hexane to degrease, mix with a high-speed homogenizer at 20°C for 30 minutes, and filter to obtain a filter residue for later use;

[0035] S20, take 20g of the filter residue and add 40mL of 0.05mol / mL phosphate buffer solution to adjust the pH to 4.5, add 60mL of crude myrosinase, stir evenly and place it at 30°C for 1.5 hours; then add the mass concentration of 95% 850mL of ethanol was extracted for 60min by ultrasonic oscillation with a vibration frequency of 40kHz and a power of 200w, and then passed through a 30-mesh sieve after standing for 45min to remove the residue of the extract to obtain the filtrate; ) to obtain the extract after suction filtration to remove impurities; ...

Embodiment 3

[0039] S10, take fresh broccoli seeds and dehydrate them at 75°C for 0.5 hours, then dry them at 55°C until the water content is lower than 8%, and then crush them to 60 mesh to obtain broccoli seed powder. Take 30 g of broccoli seed powder as a raw material, add 900 mL of n-hexane to degrease, mix with a high-speed homogenizer at 30°C for 45 minutes, filter to obtain a filter residue for later use;

[0040] S20, take 10 g of the filter residue and add 25 mL of 0.05 mol / mL phosphate buffer solution to adjust the pH to 5, add 30 mL of crude glucuronidase, stir well, place it at 40°C for 0.5 hours for enzymatic hydrolysis; then add a mass concentration of 95% 440mL of ethanol was extracted for 10min by ultrasonic oscillation with a vibration frequency of 40kHz and a power of 200w, then passed through a 30-mesh sieve after standing for 40min, and the residue of the extract was removed to obtain the filtrate; ) to obtain the extract after suction filtration to remove impurities; ...

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PUM

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Abstract

The invention relates to a method for extracting high-purity sulforaphane by taking broccoli seeds as a raw material. The method comprises the steps of degreasing the crushed broccoli seeds by using normal hexane, filtering, adjusting the pH value of a water solution to 4.5-5.5, carrying out enzymolysis, extracting dichloromethane, and finally carrying out column chromatography on silica gel, purification and the like to obtain a final product. The method is high in extraction rate and product purify. The method has the advantages of being high in extraction rate, simple in process, stable in product quality, easy in industrial production, etc.

Description

technical field [0001] The invention relates to an extraction method for extracting effective components in plants, in particular to a method for extracting sulforaphane. Background technique [0002] Epidemiological studies have shown that eating cruciferous Brassica vegetables, such as broccoli, collard greens, Brussels sprouts, cauliflower, and cabbage, can prevent pancreatic cancer, lung cancer, rectal cancer, breast cancer, and prostate cancer. This is mainly related to sulforaphane, the hydrolysis product of glucosinolates (glucosinolates) in Brassica vegetables. [0003] Sulforaphane, (1-isothiocyanato-4-methylsulfiny butane, l-isothiocyanato-4-methylsulfiny butane, sulfbraphane), molecular weight 177.3, molecular formula C 6 h 11 S 2 NO. It is produced by enzymatic hydrolysis of glucoraphanin (a type of glucosinolate), and acts by regulating the metabolism of carcinogens. It stimulates the body's self-anti-cancer mechanism by acting on the "Phase two enzymes" in ...

Claims

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Application Information

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IPC IPC(8): C07C331/22C12P13/00
CPCC07C331/22C12P13/00
Inventor 徐金蝶
Owner 徐金蝶
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