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A method for detecting clenbuterol hydrochloride residues based on a blood glucose meter

A technology of clenbuterol hydrochloride and blood glucose meter, which is applied in the field of detection of clenbuterol hydrochloride residues, can solve the problem of high operator requirements, poor portability and operability of electrochemical workstation, and limited amount of coated antigen problems such as high sensitivity, improved enrichment efficiency and impurity purification efficiency, and low detection cost

Active Publication Date: 2019-11-19
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method needs to go through a complicated electrode modification process, which has high requirements for operators, and the electrode as a solid phase carrier belongs to a single reaction interface, the amount of coated antigen is limited, and the portability and operability of the electrochemical workstation are not strong.

Method used

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  • A method for detecting clenbuterol hydrochloride residues based on a blood glucose meter
  • A method for detecting clenbuterol hydrochloride residues based on a blood glucose meter
  • A method for detecting clenbuterol hydrochloride residues based on a blood glucose meter

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Embodiment 1

[0028] A method for detecting clenbuterol hydrochloride residues based on a blood glucose meter, the flow chart is shown in figure 1 ,Specific steps are as follows:

[0029] 1) Preparation of immunomagnetic bead probes

[0030] a. Add 200 μL MES solution (15 mM, pH 6.0) containing 5 mg / mL EDC to 2 mg carboxyl magnetic beads, activate at room temperature for 30 min, and magnetically separate the supernatant;

[0031] b. Add 500 μL MES solution containing 0.1 mg CLB-BSA, react overnight at room temperature, and magnetically separate the supernatant;

[0032] c. Add PBS (0.01M, pH 6.0) solution containing 2% OVA, block at 37°C for 2 hours, and magnetically separate the supernatant to obtain immunomagnetic beads;

[0033] d. Add PBS solution containing 0.1% OVA to resuspend the magnetic beads (magnetic bead concentration: 1 mg / mL), and store in a refrigerator at 4°C until use.

[0034] 2) Preparation of colloidal gold / invertase / secondary antibody complex (ie enzyme-labeled seco...

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Abstract

The invention discloses a method for detecting clenbuterol hydrochloride residues based on a blood glucose meter, which belongs to the technical field of biological detection and immunosensors. The method comprises the following steps: (1) coating a clenbuterol hydrochloride complete antigen on the surfaces of magnetic beads, and blocking to obtain an immunomagnetic bead probe; (2) adding a secondary antibody to a colloidal gold solution, mixing, adding an invertase to obtain an enzyme-labeled secondary antibody; (3) adding the immunomagnetic bead probe and anti-clenbuterol hydrochloride antibody to to-be-detected solution, performing immunological reaction and magnetic separation to obtain a magnetic bead / antigen-antibody immune complex; and then adding the enzyme-labeled secondary antibody, performing reaction and magnetic separation to obtain a magnetic bead / antigen-antibody / enzyme-labeled secondary antibody immune complex; and (4) adding a sucrose solution to the magnetic bead / antigen-antibody / enzyme-labeled secondary antibody immune complex, performing hydrolysis reaction, detecting a glucose signal value with a blood glucose meter, and obtaining the clenbuterol hydrochloride residual level in the to-be-detected solution according to a standard curve. The method disclosed by the invention has the characteristics of simplicity in operation, fastness and accuracy in detection, high sensitivity, and the like.

Description

technical field [0001] The invention relates to a method for detecting clenbuterol hydrochloride residues based on a blood glucose meter, and belongs to the technical field of biological detection and immune sensors. Background technique [0002] Clenbuterol hydrochloride (CLB), commonly known as clenbuterol, is a potent and selective β-agonist widely used in the prevention and treatment of bronchitis, asthmatic chronic bronchitis and other respiratory diseases and obstetrics. Studies have found that when it is added to feed 5 to 10 times higher than the clinical application dose, it can lead to increased fat catabolism and protein synthesis in animals, and significantly improve the lean meat rate of carcasses. However, once a person eats meat and its products containing a large amount of CLB residues for a long time, mild symptoms such as dizziness, nausea, tremors, and muscle tremors will occur. In addition, CLB can cause insufficient glycolysis in the animal muscle and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34
CPCC12Q1/34
Inventor 康怀彬李芳张瑞华邹良亮蔡超奇
Owner HENAN UNIV OF SCI & TECH
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