Method for detecting clenbuterol hydrochloride residues based on blood glucose meter
A technology of clenbuterol hydrochloride and blood glucose meter, which is applied in the field of detecting clenbuterol hydrochloride residues, can solve the problem of high operator requirements, poor portability and operability of electrochemical workstations, and limited amount of coated antigens and other problems, to achieve the effects of high sensitivity, improved enrichment efficiency and impurity purification efficiency, and low detection cost
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[0028] A method for detecting clenbuterol hydrochloride residues based on a blood glucose meter, the flow chart is shown in figure 1 ,Specific steps are as follows:
[0029] 1) Preparation of immunomagnetic bead probes
[0030] a. Add 200 μL MES solution (15 mM, pH 6.0) containing 5 mg / mL EDC to 2 mg carboxyl magnetic beads, activate at room temperature for 30 min, and magnetically separate the supernatant;
[0031] b. Add 500 μL MES solution containing 0.1 mg CLB-BSA, react overnight at room temperature, and magnetically separate the supernatant;
[0032] c. Add PBS (0.01M, pH 6.0) solution containing 2% OVA, block at 37°C for 2 hours, and magnetically separate the supernatant to obtain immunomagnetic beads;
[0033] d. Add PBS solution containing 0.1% OVA to resuspend the magnetic beads (magnetic bead concentration: 1 mg / mL), and store in a refrigerator at 4°C until use.
[0034] 2) Preparation of colloidal gold / invertase / secondary antibody complex (ie enzyme-labeled seco...
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