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Lipid compound for auxiliary diagnosis of breast cancer and related kit thereof

A compound and breast cancer technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as insufficient research, lack of good diagnostic efficiency of a single lipid species, and no disclosure of lipid compounds for breast cancer, etc., to achieve Strong pertinence, high detection accuracy, and high clinical value

Inactive Publication Date: 2017-01-25
上海汇真生物科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing lipid-related studies only focus on the difference between the lipid content of cancer patients and normal people, and only a few of them involve patients with benign breast diseases, among which Yang et al. Comprehensive evaluation of plasma lipid profiles in disease patients, only involving 5 cases of breast cancer and 6 cases of benign patients, the small sample size is insufficient to study the problem
Furthermore, a single lipid species does not have good diagnostic efficiency to distinguish benign from breast cancer patients
[0006] At present, there is no published standard substance of lipid compounds for auxiliary diagnosis of breast cancer at home and abroad, especially a group of effective standard substances involved in distinguishing high-risk women from breast cancer patients

Method used

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  • Lipid compound for auxiliary diagnosis of breast cancer and related kit thereof
  • Lipid compound for auxiliary diagnosis of breast cancer and related kit thereof
  • Lipid compound for auxiliary diagnosis of breast cancer and related kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Patient Selection

[0032] The training set includes 39 breast cancer and 51 benign breast disease samples obtained from our breast cancer repository. Sample selection should follow the following criteria: (1) all patients were diagnosed and confirmed by pathology; (2) according to the clinical staging method, breast cancer patients should be in the early stage (stage 0, I, II); (3) patients had no other Diseases that may affect lipid metabolism, such as hyperlipidemia, diabetes, and other cancers; (4) All patients were female; (5) Patients did not receive preoperative adjuvant chemotherapy or radiotherapy. Benign breast lesions are defined as hyperplasia, fibromas, cysts, and some unidentified breast diagnoses. Control blood samples were obtained from healthy females with no history of malignancy and inflammation.

[0033] According to these criteria, plasma samples from 45 patients with early breast cancer (stage 0-II) and 59 patients with benign breast dis...

Embodiment 2

[0034] Embodiment 2: Collection of plasma samples

[0035] Plasma samples were collected after patients had fasted for at least 12 hours. Briefly, plasma collection means that blood is collected into a vacuum blood collection EDTA anticoagulant tube after venipuncture, centrifuged at 4°C and 2600g for 10 minutes within 2 hours, the supernatant is taken, and centrifuged for the second time in the same way, and the plasma is divided into 0.5ml / aliquot Store at -80°C. All plasma samples were shipped on dry ice to the Kansas Lipidomics Research Center for lipid analysis.

[0036] Table 1. Information table of patients with breast cancer and benign breast diseases in the training set and validation set

[0037]

[0038]

Embodiment 3

[0039] Example 3: LC-ESI-MS / MS lipid profile

[0040] For each sample analysis, 3 μL of plasma was centrifuged for 20 minutes prior to detection to globularize proteins in surface tube units. In addition, in order to accurately identify all lipids, it is necessary to accurately add internal standards, wherein each type of lipid substance uses two internal standards. After centrifugation for 20 min, lipid extracts were redissolved in solvent and injected into HPLC. The solvent mixing ratio was chloroform / methanol / 300 mM ammonium acetate (μL) 360 / 840 / 44 dissolved in water. All solvents were HPLC grade.

[0041] Lipids were analyzed by triple quadrupole LC-ESI-MS / MS (API 4000, Applied Biosystems, Foster City, CA), which could distinguish their polar heads and chain lengths. Sample introduction is by electrospray ionization (ESI). It reduces ionization suppression effects caused by spectral congestion. Complex lipids generate simple charged ions by ESI, and fragment ions can ...

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Abstract

The invention relates to a breast cancer blood plasma lipid molecular marker, a kit containing the compound and a method for carrying out qualitative or quantitative detection on the lipid compound. Lipidomics identifies that the expression difference of fifteen lipid molecules can influence the occurrence and development of the breast cancer. The plasma level of the fifteen lipid molecules is proportional to the morbidity rate of the breast cancer, and detection on the breast cancer can be more conveniently and effectively realized on the molecular level by determining the plasma level of a specific lipid molecule.

Description

technical field [0001] The invention belongs to the field of in vitro detection of biomarkers, in particular to a group of lipid molecular markers for distinguishing high-risk patients from breast cancer patients, in particular to a group of lipid compounds for auxiliary diagnosis of breast cancer, a kit containing the compound, And a method for qualitatively or quantitatively detecting the lipid compound. Background technique [0002] Breast cancer is the most common and deadly cancer in women and the second leading cause of cancer death in women. According to the American Cancer Society (ACS), there were 231,840 newly diagnosed cases of breast cancer in 2015, accounting for 29% of all newly diagnosed female cancer patients. In addition, 40,290 breast cancer deaths accounted for 15% of female cancer-related deaths, posing a huge threat to women's health. [0003] At present, the clinical diagnosis of breast cancer is relatively complicated, mainly including near-infrared ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57415
Inventor 邓友平王红卫
Owner 上海汇真生物科技有限公司
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