Fermentation production method of locust green muscardine fungus, and green muscardine fungus solid culture medium

Abstract

The invention provides a fermentation production method of locust green muscardine fungus. The fermentation production method of the locust green muscardine fungus comprises the following steps: 1) activating the culture of the locust green muscardine fungus; 2) inoculating the locust green muscardine fungus active in the step 1) into a locust green muscardine fungus liquid culture medium, and performing liquid culture and stopping culture after performing culture for 30 to 60 hours to obtain locust green muscardine fungus culture liquid; 3) inoculating the locust green muscardine fungus culture liquid obtained in the step 2) into a locust green muscardine fungus solid culture medium and performing solid culture to obtain the locust green muscardine fungus. The liquid culture medium can generate strong bacteria so as to generate more spores, and the locust spores can be germinated in the liquid culture medium, so that a large amount of bacteria can be generated quickly in the solid culture stage, and the generated spores are 10 billion to 100 billion pieces / g.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation production method of Metarhizium anisopliae and a solid medium for Metarhizium anisopliae. Background technique [0002] my country is a large agricultural country, and agriculture is the foundation of the national economy, the source of human food and clothing, and the foundation of survival. Therefore, the development of agriculture directly affects the overall development of the national economy. However, in the process of agricultural production, insect pests often seriously affect the normal growth of crops, reduce the yield of crops, and bring huge economic losses to growers. Therefore, it is necessary to effectively prevent and control crop pests to ensure food security and the economic interests of growers. [0003] There are many kinds of pests that attack crops today, the most important ones are insects, Lepidoptera pests, Hymenoptera pest...

AI Technical Summary

Problems solved by technology

In recent years, researchers at home and abroad have conducted research on Metarhizium anisopliae, so they have achieved research results in biology, ecology, etc., and have shown great potential in biological control and broad market prospects. Although traditional fungal fermentation The method can realize industrialized production, but there are problems such as long fermentation time, high cost and low yield