Fermentation production method of locust green muscardine fungus, and green muscardine fungus solid culture medium

The technology of a solid culture medium and production method is applied to the fermentation production method of Metarhizium locust and the field of Metarhizium anisopliae solid culture medium, and can solve the problems of long fermentation time, high cost, low yield and the like

Inactive Publication Date: 2017-02-01
JIANGXI TIANXIANG GENERAL AVIATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers at home and abroad have conducted research on Metarhizium anisopliae, so they have achieved research results in biology, ecology, etc., and have shown great potential in biologica

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Preparation of Metarhizium anisopliae culture solution: Inoculate the preserved Metarhizium anisopliae strains into eggplant-shaped bottles equipped with nutrient agar medium, and culture them at 26°C for 12 hours for activation; The inoculum size of % is inoculated in the liquid culture medium after 121 ℃ of sterilization 35min, carries out liquid culture in fermenter, and liquid culture medium comprises the component of following mass content: soybean powder 30g / L, sucrose 30g / L, flour 0.05 g / L, sweet potato powder 0.05g / L, disodium hydrogen phosphate 0.0002g / L, and the balance is water. The specific culture conditions are: temperature is 26°C; pH value is 6.9; It was 1V / V min; the culture was stopped after 46 hours of cultivation to obtain the culture solution of Metarhizium anisopliae;

[0051] The preparation of solid Metarhizium anisopliae: the obtained Metarhizium anisopliae culture solution is inoculated in a solid medium with an inoculum size of 20%, and distil...

Embodiment 2

[0053] Preparation of Metarhizium anisopliae culture solution: Inoculate the preserved Metarhizium anisopliae strains into eggplant-shaped bottles equipped with nutrient agar medium, and culture them at 22°C for 12 hours for activation; The inoculum size of % is inoculated in the liquid medium after 121 ℃ of sterilizations 35min, carries out liquid culture in fermenter, and liquid medium comprises the component of following mass content: soybean powder 20g / L, sucrose 40g / L, flour 0.03 g / L, sweet potato powder 0.07g / L, disodium hydrogen phosphate 0.0002g / L, and the balance is water. The specific culture conditions are: temperature is 22°C; pH value is 6.6; It was 1V / V min; after cultivating for 60h, stop culturing to obtain Metarhizium anisopliae culture fluid;

[0054] The preparation of solid Metarhizium anisopliae: the obtained Metarhizium anisopliae culture solution is inoculated in a solid medium with an inoculum size of 15%, distilled water is added in the solid medium so...

Embodiment 3

[0056] Preparation of Metarhizium anisopliae culture solution: Inoculate the preserved Metarhizium anisopliae strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 22°C for 14 hours for activation; The inoculum size of % is inoculated in the liquid culture medium after 121 ℃ of sterilization 35min, carries out liquid culture in fermenter, and liquid culture medium comprises the component of following mass content: soybean powder 40g / L, sucrose 20g / L, flour 0.07 g / L, sweet potato powder 0.03g / L, disodium hydrogen phosphate 0.0002g / L, and the balance is water. The specific culture conditions are: temperature is 30°C; pH value is 7.2; It was 1V / V min; after cultivating for 30h, stop culturing to obtain Metarhizium anisopliae culture fluid;

[0057] The preparation of solid Metarhizium anisopliae: the obtained Metarhizium anisopliae culture solution is inoculated in a solid medium with an inoculum size of 25%, and distilled water is added i...

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PUM

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Abstract

The invention provides a fermentation production method of locust green muscardine fungus. The fermentation production method of the locust green muscardine fungus comprises the following steps: 1) activating the culture of the locust green muscardine fungus; 2) inoculating the locust green muscardine fungus active in the step 1) into a locust green muscardine fungus liquid culture medium, and performing liquid culture and stopping culture after performing culture for 30 to 60 hours to obtain locust green muscardine fungus culture liquid; 3) inoculating the locust green muscardine fungus culture liquid obtained in the step 2) into a locust green muscardine fungus solid culture medium and performing solid culture to obtain the locust green muscardine fungus. The liquid culture medium can generate strong bacteria so as to generate more spores, and the locust spores can be germinated in the liquid culture medium, so that a large amount of bacteria can be generated quickly in the solid culture stage, and the generated spores are 10 billion to 100 billion pieces/g.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation production method of Metarhizium anisopliae and a solid medium for Metarhizium anisopliae. Background technique [0002] my country is a large agricultural country, and agriculture is the foundation of the national economy, the source of human food and clothing, and the foundation of survival. Therefore, the development of agriculture directly affects the overall development of the national economy. However, in the process of agricultural production, insect pests often seriously affect the normal growth of crops, reduce the yield of crops, and bring huge economic losses to growers. Therefore, it is necessary to effectively prevent and control crop pests to ensure food security and the economic interests of growers. [0003] There are many kinds of pests that attack crops today, the most important ones are insects, Lepidoptera pests, Hymenoptera pest...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 李肖宇梁小文石峥曾升华石仕福吴俊杰罗建辉李英武罗双燕王根豪严艺波
Owner JIANGXI TIANXIANG GENERAL AVIATION CO LTD
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