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Cow phytoplasma substrate

A technology of Mycoplasma bovis, culture medium, applied in bacterial antigen components, microorganism-based methods, microorganisms, etc., to achieve the effect of increasing the titer of viable bacteria, promoting microbial metabolism, and reducing the amount of serum

Active Publication Date: 2017-02-15
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, since the above-mentioned patents are used to cultivate Mycoplasma hyopneumoniae and Mycoplasma capricum subspecies, the titers of Mycoplasma bovis are not higher than 10. 9 CCU / ml; In addition, the amount of horse serum required by the above-mentioned patents is relatively large, and the content of horse serum is all at 10% and above; important question

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  • Cow phytoplasma substrate

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Embodiment 1

[0027] 1, the preparation of culture medium of the present invention:

[0028] Basal medium:

[0029] (1) PPLO Broth 22.0 g

[0030] (2) Sodium pyruvate 5.0 g

[0031] (3) Glucose 6.0 g

[0032] (4) 2.0 ml of phenol red with a mass concentration of 1%

[0033] (5) 750ml of deionized water.

[0034] Sterilize at 115°C for 20 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to prepare the medium of the present invention.

[0035] Auxiliary medium:

[0036] (6) 110 ml of fresh yeast extract with a mass concentration of 25%

[0037] (7) 17.0 ml of L-glutamine with a mass concentration of 3%

[0038] (8) 5.0 ml of L-cysteine ​​with a mass concentration of 10%

[0039] (9) 33.0 ml of hydrolyzed milk protein with a mass concentration of 15%

[0040] (10) Penicillin (200,000 IU / ml) 0.25ml

[0041] (11) Sterile horse serum 50 ml

[0042] Dissolve the ingredients (1) to (4) in the basal medium one by one into 750ml of deionized wa...

Embodiment 2

[0048] The preparation of substratum of the present invention:

[0049] Basal medium:

[0050] (1) PPLO Broth 22.0 g

[0051] (2) Sodium pyruvate 5.0 g

[0052] (3) Glucose 8.0 g

[0053] (4) 1% phenol red 2.0 ml

[0054] (5) 750ml of deionized water.

[0055] Sterilize at 115°C for 20 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to prepare the medium of the present invention.

[0056] Auxiliary medium:

[0057] (6) 120 ml of 25% fresh yeast extract

[0058] (7) 17.0 ml of L-glutamine with a mass concentration of 3%

[0059] (8) 5.0 ml of L-cysteine ​​with a mass concentration of 10%

[0060] (9) The mass concentration is 15% hydrolyzed milk protein 33.0 ml

[0061] (10) Penicillin (200,000 IU / ml) 0.25ml

[0062] (11) Sterile horse serum 50 ml

[0063] Dissolve the ingredients (1) to (4) in the basal medium one by one into 750ml of deionized water (5), autoclave at 115°C for 20 minutes, and let it cool to room temper...

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Abstract

The invention discloses a cow phytoplasma substrate and preparation method thereof, and belongs to the technical field of veterinary biology. The cow phytoplasma substrate constitutes a basic substrate and an assistant substrate, wherein, the substrate is constituted from PPLO broth, sodium pyruvate, glucose, fresh yeast extract, L-glutamine, L-cysteine, water solvent milk protein, horse serum, penicillin, phenol red, and deionized water. The substrate can reduce the use level of serum, and increase evidently the active bacterium titer of cow phytoplasma, thus establishing a foundation for studying quality cow phytoplasma vaccine.

Description

technical field [0001] The present invention relates to a culture medium for Mycoplasma bovis and a preparation method thereof. Specifically, its components include: PPLO broth, sodium pyruvate, glucose, fresh yeast extract, horse serum, penicillin, phenol red, deionized Aqueous Mycoplasma bovis culture medium and its preparation method. Background technique [0002] Mycoplasma bovis (M.bovis) can cause bovine pneumonia, arthritis, mastitis, keratoconjunctivitis, otitis, reproductive tract inflammation, miscarriage and infertility and other diseases. The disease caused by this pathogen is prevalent all over the world and causes serious economic losses. Mycoplasma bovis is the main cause of bovine respiratory disease syndrome. Since Mycoplasma bovis was first isolated from beef cattle with outbreaks of necrotizing pneumonia in Hubei Province in 2008, there have been reports of cases of Mycoplasma bovis in many provinces and cities across the country. Now Mycoplasma bovis ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/02A61P31/04A61P11/00C12R1/35
Inventor 陈胜利储岳峰郝华芳赵萍刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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