Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein

A whey protein and casein technology, applied in the field of milk powder processing, can solve the problems of reducing the probability of contact between digestive enzymes and protein, reducing protein digestibility, etc., and achieves good application prospects, social and economic benefits, and efficient separation effects.

Active Publication Date: 2017-02-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isoelectric point of bovine milk casein is about 4.6, and it accounts for a relatively high proportion of the total milk protein, so it is easy to form a dense flocculation ...

Method used

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  • Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein
  • Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein
  • Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein

Examples

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Effect test

Embodiment example 1

[0013] A method for preparing a novel functional milk protein ingredient rich in β-casein and whey protein by using skim milk powder as a raw material through low-temperature microfiltration technology. The specific process steps are as follows:

[0014] Take 3 parts of 15kg skimmed milk powder, add 150L of water respectively, stir and dissolve at 50°C for 1 hour, add the obtained complex solution into the liquid storage tank of the microfiltration system, cool its temperature to about 4°C through a low-temperature circulator, and After the equilibrium was stable at this temperature for 1 hour, ceramic membranes with three different pore sizes of 30, 50, and 100 nm were used for microfiltration and concentration. During the microfiltration process, the temperature was still controlled at 4 ° C, and the microfiltration was stopped when the volume of the permeate reached 100 L. The obtained permeate is concentrated by ultrafiltration using an ultrafiltration membrane with a molec...

Embodiment example 2

[0017] A new functional milk protein ingredient rich in β-casein and whey protein is prepared by low-temperature microfiltration technology using whole milk as raw material. The specific process steps are as follows:

[0018] Take 3 parts of 300L pasteurized whole milk, use a milk fat separator to remove fat, add the obtained skim milk to the liquid storage tank of the microfiltration system, and cool the temperature to 4, 25, and 50°C respectively through a low-temperature circulator After equilibrating and stabilizing at the corresponding temperature for 1 hour, use a ceramic membrane with a pore size of 30nm for microfiltration and concentration. During the microfiltration process, the corresponding temperature is still controlled, and the microfiltration is stopped when the concentration ratio is 3. The obtained permeate is concentrated by ultrafiltration using an ultrafiltration membrane with a molecular weight cut-off of 5kDa at room temperature to remove lactose and mine...

Embodiment example 3

[0021] A new functional milk protein ingredient rich in β-casein and whey protein is prepared by low-temperature microfiltration technology using skim milk as raw material. The specific process steps are as follows:

[0022] Take 2 parts of 150L pasteurized skim milk, and use a microfiltration ceramic membrane with a pore size of 30nm to separate at 4°C. The whole process is divided into 1 microfiltration and 4 diafiltrations: the first microfiltration is the concentration and separation of the original skim milk, that is, 150L skim milk is concentrated 3 times, and 100L permeate is collected; the diafiltration operation is at the end of the microfiltration Finally, add water until the original volume of the feed solution is 150L, ​​and continue to concentrate 3 times. Collect the permeate obtained in each stage of separation, and use ultrafiltration membrane with a molecular weight cut-off of 10kDa to carry out ultrafiltration and concentration at room temperature to remove l...

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Abstract

The invention relates to a method for separating cow milk beta-casein and whey protein at the low temperature through microfiltration to simulate composition of human lactoprotein. Skim milk, whole milk, dried skim milk, concentrated milk protein powder and the like are taken as raw materials, decalcification pretreatment is performed with the adoption of citrate, glucono-delta-lactone and other chelating agents or an acidifier, microfiltration and filter wash are performed with the adoption of a ceramic membrane or a polyethersulfone membrane and the like under the low-temperature (0-15 DEG C) condition, beta-casein and whey protein are selectively enriched into a permeating liquid, the permeating liquid is subjected to spray drying and dewatering after lactose and mineral substances are removed through ultrafiltration and concentration, and high-protein powder containing rich beta-casein and whey protein is prepared. An adopted technical process has the characteristics of being environment-friendly, efficient, high in operability, good in safety and the like and is suitable for large-scale industrial production. The obtained compound protein powder can serve a novel functional lactoprotein ingredient to replace whey protein and is used for research and development of infant food such as formula milk powder and the like, so that the protein molecule composition of the compound protein powder is closer to that of human lactoprotein.

Description

technical field [0001] The invention relates to a method for separating bovine milk β-casein and whey protein by low-temperature microfiltration to simulate human milk protein composition, and belongs to the field of milk powder processing. Background technique [0002] With the development of society and the improvement of people's living standards, more and more people will choose to replace breast milk with infant formula milk powder made of cow's milk protein under the premise of insufficient breast-feeding or poor mother's physique. Both bovine milk protein and human milk protein mainly contain casein and whey protein, but there are still big differences in specific protein composition and molecular ratio. The ratio of casein to whey protein in cow's milk protein is about 80:20, while the ratio of casein to whey protein in human milk protein is about 40:60. At present, the development of breastmilkization of infant formula milk powder is mainly focused on increasing th...

Claims

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Application Information

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IPC IPC(8): A23J1/20A23C9/15A23C9/16
CPCA23C9/1512A23C9/16A23J1/20A23J1/202
Inventor 周鹏刘大松李珺珂余韵刘小鸣
Owner JIANGNAN UNIV
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