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A kind of biocontrol actinomycetes strain and application thereof for preventing and treating soybean blight

A technology of actinomycetes and biocontrol agents, applied in the direction of application, bacteria, and biocides, can solve problems such as unsatisfactory chemical control effects and environmental pollution, and achieve the goal of preventing and controlling soybean diseases, reducing agricultural pollution, and facilitating industrial production Effect

Active Publication Date: 2019-06-28
INST OF PLANT PROTECTION FAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a biocontrol actinomycetes for effectively preventing and treating soybean blight and its application in view of the serious occurrence of soybean blight, the area is expanding year by year, the effect of chemical control is not ideal, and the problem of environmental pollution is also provided.

Method used

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  • A kind of biocontrol actinomycetes strain and application thereof for preventing and treating soybean blight
  • A kind of biocontrol actinomycetes strain and application thereof for preventing and treating soybean blight
  • A kind of biocontrol actinomycetes strain and application thereof for preventing and treating soybean blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation of antagonistic actinomycetes

[0021] The specific process is as follows:

[0022] 1. Collection of soil samples

[0023] The rhizosphere soil at a depth of 5-10 cm from different soybean plots was collected from Nanyuan Village, Bangshan Town, Longhai City, Fujian Province, divided into packages and marked, and brought back to the laboratory, and separated after natural air drying.

[0024] , Isolation of Actinomycetes

[0025] Separation was performed using the plate dilution method. Weigh 10g of the soil sample, pour it into a conical flask equipped with small glass beads and 90ml of sterile water, shake it for 30min, then let it stand for 5 minutes, dilute it by 10 times successively, and prepare 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 0.1ml of the suspension of different concentrations was added to Gao's No. 1 medium (adding K with a final concentration of 50mg / L 2 GrO 7 ) plate, spread evenly and place it at 28°C for observation. After 5-7 ...

Embodiment 2

[0026] Example 2 Identification of bacterial strain St3

[0027] 1. Observation of morphological characteristics

[0028] Strain-inoculate the strain St3 on Gao's No. 1 medium, insert a sterile cover glass at an angle of 45 degrees, and culture it at 28°C for 7 days, then take out the cover glass and observe the growth of hyphae and spores under an optical microscope and an electron microscope. Morphological characteristics. It was found that the spore filaments of strain St3 were helical, often in clusters, and the spore filaments were broken into spores, spores were oval, and the surface was rough ( figure 1 and figure 2 ).

[0029] , Cultivate characteristic observation

[0030] Using the 8 kinds of media recommended by the "Streptomyces Identification Manual", culture the strain St3 at 28°C for 7-10 days, observe the growth of the bacteria, and record the color of the aerial hyphae and basal hyphae and whether soluble pigments are produced. Strain St3 grew well on G...

Embodiment 3

[0035] The optimization of embodiment 3 St3 bacterial strain fermentation technology

[0036] The above strains were inoculated in Gaoshi No. 1 liquid medium at 28 °C, 180 r / min, and cultured for 2 days to make seed liquid. Take 6ml of seed solution and inoculate it in the fermentation medium. Through the screening of carbon and nitrogen sources, single factor test and orthogonal test of nutritional conditions, the optimal fermentation formula of the strain was obtained: starch 2wt.%, glucose 1.5 wt.%, soybean powder 2wt.%, NaCl 0.3 wt.%, CaCO 3 0.2 wt.%, the rest is water. Through exploring the culture conditions, the optimal fermentation conditions were obtained: inoculum size 10% (v / v), bottling volume 60mL / 250mL, initial pH value of the fermentation medium 8.0, 28°C, 200 r / min, and culture for 5 days , resulting in a concentration of 10 8 St3 strain fermentation broth of CFU / ml.

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Abstract

The invention provides a biocontrol actinomycete strain for preventing and treating soybean blight and application thereof, belonging to the technical field of crop disease prevention and control. Described biological control actinomycetes bacterial strain is Streptomyces graminearum ( Streptomyces graminearus ) St3, which was registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on March 24, 2014, with the preservation number CGMCCNO.8961. The strain inhibits the growth of Phytophthora soybean by producing active substances, and shows better control effect on soybean blight. When applied, it is used to irrigate the roots of soybeans at the seedling stage or adult plant stage. The bacterial agent can also be mixed with organic fertilizer to make bacterial fertilizer. The bacterial strain St3 of the present invention is obtained from the soybean rhizosphere soil, is harmoniously compatible with the soil ecology, is non-toxic and non-pathogenic, and therefore has a good application prospect in the biological control of soybean blight.

Description

technical field [0001] The invention relates to a biocontrol actinomycete strain for preventing and treating soybean blight and an application thereof, belonging to the technical field of crop disease prevention and control. Background technique [0002] Soybean blight is caused by Phytophthora sojae ( Phytophthora sojae ) is one of the devastating diseases in soybean production caused by infestation. The disease can occur throughout the growth period of soybeans, but it is most common at the seedling stage. If it is accompanied by rain, it will easily cause a large area of ​​the disease The yield is severely reduced or even completely eliminated, and the control is difficult, which seriously affects the development of my country's soybean industry and causes major economic losses. [0003] At present, the main control methods of soybean blight are cultivating disease-resistant varieties and spraying chemical pesticides. However, populations of Phytophthora sojae can change...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 陈庆河李本金刘裴清王荣波翁启勇
Owner INST OF PLANT PROTECTION FAAS
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