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Preparation method of enteromorpha protease

A protease inhibitor and protease technology, applied in the field of plant protease, to achieve the effects of low raw material price, reduced energy consumption, and simple preparation process

Inactive Publication Date: 2017-02-22
威海红印食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Also do not have a kind of preparation method of enteromorpha protease in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of enteromorpha protease, comprising the following steps:

[0033] (1) Select Enteromorpha and clean the salvaged Enteromorpha;

[0034] (2) Cut the salvaged Enteromorpha, then add a2-macroglobulin to squeeze the juice to obtain Enteromorpha juice; 100 grams of Enteromorpha juice contains 1 gram of a2-macroglobulin;

[0035] (3) Put 100 grams of Enteromorpha juice into a masher, add extract and buffer to the masher, mash and extract and filter the suspension, and the obtained filtrate is the crude extract of Enteromorpha protease;

[0036] The preparation method of the extract: add 0.5 g of NaCI to 1 L of distilled water, configure the NaCI solution, and add 0.3 g of potassium chloride to the configured sodium chloride NaCI solution;

[0037] The preparation method of the buffer solution: add 1L of distilled water to 1.5g of trimethylolmethane to prepare a trimethylolmethane solution;

[0038] (4) Add solid ammonium sulfate to the crude extract at...

Embodiment 2

[0043] A preparation method of enteromorpha protease, comprising the following steps:

[0044] (1) Select Enteromorpha, and clean the salvaged Enteromorpha; the selected Enteromorpha: after salvage to use ≤ 2 days, after salvage at natural temperature, the processing temperature of Enteromorpha is 30°C; wash Enteromorpha with 100kpa high-pressure water to remove Sand and gravel, time 20-30 minutes.

[0045] (2) Perform natural water leakage treatment on the cleaned Enteromorpha:

[0046] The Enteromorpha after washing is flatly piled up on the leaking frame, each layer of the leaking frame is provided with a water receiving chassis, the thickness of each layer of Enteromorpha is 15-25cm, and the length and width of the cut Enteromorpha are 1-1.5cm; Enteromorpha cut, then add B.M. comprehensive enzyme tablet to squeeze out the juice to obtain Enteromorpha juice; the amount of B.M. comprehensive enzyme tablet is 2% of the weight of Enteromorpha juice, that is, add 2 grams of pr...

Embodiment 3

[0057] A preparation method of enteromorpha protease, comprising the following steps:

[0058] (1) Select Enteromorpha, and clean the salvaged Enteromorpha; the selected Enteromorpha: after salvage to use ≤ 2 days, after salvage at natural temperature, the processing temperature of Enteromorpha is 20°C; wash Enteromorpha with 100kpa high-pressure water to remove Sand and gravel, time 20-30 minutes.

[0059] (2) Perform natural water leakage treatment on the cleaned Enteromorpha:

[0060] The Enteromorpha after washing is flatly piled up on the leaking frame, each layer of the leaking frame is provided with a water receiving chassis, the thickness of each layer of Enteromorpha is 15-25cm, and the length and width of the cut Enteromorpha are 1-1.5cm; Enteromorpha is cut, then add Rocheprotease and squeeze the juice to obtain Enteromorpha juice; the addition of Rocheprotease is 2% of the weight of Enteromorpha juice, that is, 100 grams of Enteromorpha juice adds 2 grams of prote...

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Abstract

The invention discloses a preparation method of enteromorpha protease. The preparation method comprises the following steps: selecting raw materials; cleaning under high pressure; shearing water leaking; adding protease inhibitor juice; adding leaching liquor and a buffer solution in a triturator and preparing coarse extraction liquid; centrifuging solid ammonium sulfate; dialyzing and desalting an acetic acid solution; adding a Roche protease inhibitor and cooling by ice bath; adding chloroprene rubber cr322 and stirring and centrifuging; purifying with sodium bicarbonate; crystallizing n-acetyl-l-a cysteine solution; freezing, centrifuging and collecting crystals; and storing finished products. Activity and purity of the enteromorpha protease are guaranteed effectively. The enteromorpha protease can be widely used in the industries of washing industry, food industry, textile industry, leather industry, pulp industry, feedstuff, petroleum and gas drilling, clinic medicines, agriculture, forestry, animal husbandry and the like, and has excellent effects of reduction of energy consumption, reduction of chemicals, speediness and environmental protection in application.

Description

technical field [0001] The invention relates to a plant protease, in particular to a preparation method of Enteromorpha protease. Background technique [0002] In recent years, plant protease has been widely used in washing industry, food industry, textile industry, leather industry, pulp industry, feed, oil and gas drilling, clinical medicine, agriculture, forestry, animal husbandry and other industries. Plant protease can reduce energy consumption, Its application has attracted more and more people's attention due to its excellent effects such as reducing chemicals and fast environmental protection. [0003] Preparation of plant protease Use plant matter as raw material to prepare protease. Commonly used plant proteases are mostly green grains, grasses, immature grains and leaves. The advantage of Enteromorpha protease is that it can be well combined with the marine economy, and the price of raw materials is low, which helps to better comprehensively utilize the raw mate...

Claims

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Application Information

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IPC IPC(8): C12N9/50
CPCC12N9/63
Inventor 迟桂丽
Owner 威海红印食品有限公司
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