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A kind of in vitro culture medium of water tower flower

A culture medium and water tower flower technology, applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of quality and yield decline, planting benefit decline, and yield reduction, so as to improve yield and quality, improve the survival rate of cultivation, The effect of strong vitality and tenacious

Active Publication Date: 2019-05-24
日照新睿招商发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional seedling breeding and propagation methods of Shuitahua have reduced yield, serious virus accumulation, species degradation, greatly reduced quality and yield, and reduced planting benefits.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A kind of in vitro culture medium of water tower flower, including three kinds of culture medium in different stages, the raw materials and component concentrations of various culture medium are as follows,

[0014] The primary medium is: on the basis of the improved White medium, first adjust the concentration of iron sulfate to 3.2mg / L, adjust the concentration of sodium sulfate to 180mg / L, then add aspartic acid 8mg / L, add indole Acetic acid 3mg / L;

[0015] The subculture medium is: on the basis of the improved White medium, first adjust the concentration of boric acid to 2.2mg / L, adjust the concentration of molybdenum oxide to 0.0005mg / L, add vitamin K1 0.8mg / L, and add indium Indoleacetic acid 1.2mg / L, add naphthaleneacetic acid 1.5mg / L;

[0016] The rooting medium is: in B 5 On the basis of the culture medium, 0.02 mg / L of ammonium molybdate and 3 mg / L of gibberellin were added, and the pH was adjusted to 7.2 with lime water.

Embodiment 2

[0018] A kind of in vitro culture medium of water tower flower, including three kinds of culture medium in different stages, the raw materials and component concentrations of various culture medium are as follows,

[0019] The primary medium is: on the basis of the improved White medium, first adjust the concentration of iron sulfate to 3.0mg / L, adjust the concentration of sodium sulfate to 160mg / L, then add aspartic acid 6mg / L, add indole Acetic acid 2mg / L;

[0020] The subculture medium is: on the basis of the improved White medium, first adjust the concentration of boric acid to 2.0mg / L, adjust the concentration of molybdenum oxide to 0.0003mg / L, add vitamin K1 0.5mg / L, and add indium Indoleacetic acid 1mg / L, add naphthaleneacetic acid 1.2mg / L;

[0021] The rooting medium is: in B 5 On the basis of the culture medium, 0.01 mg / L of ammonium molybdate and 2 mg / L of gibberellin were added, and the pH was adjusted to 7.2 with lime water.

Embodiment 3

[0023] A kind of in vitro culture medium of water tower flower, including three kinds of culture medium in different stages, the raw materials and component concentrations of various culture medium are as follows,

[0024] The primary medium is: on the basis of the improved White medium, first adjust the concentration of iron sulfate to 3.0-3.2 mg / L, adjust the concentration of sodium sulfate to 160-180 mg / L, and then add 6-8 mg of aspartic acid / L, add indole acetic acid 2~3mg / L;

[0025] The subculture medium is: on the basis of the improved White medium, first adjust the concentration of boric acid to 2.0-2.2 mg / L, adjust the concentration of molybdenum oxide to 0.0003-0.0005 mg / L, and add vitamin K1 0.5-0.8 mg / L, add indole acetic acid 1~1.2 mg / L, add naphthalene acetic acid 1.2~1.5 mg / L;

[0026] The rooting medium is: in B 5 On the basis of the medium, additionally add ammonium molybdate 0.01-0.02 mg / L, add gibberellin 2-3 mg / L, and adjust the pH to 7.2 with lime water...

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PUM

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Abstract

An in-vitro culture medium of billbergia pyramidalis, comprising three culture mediums in different stages, wherein the raw materials and the component concentration of every culture medium are as below: an initial medium is to adjust concentration of ferric sulfate to 3.0-3.2 mg / L on the basis of improved White culture medium, adjust the concentration of sodium sulfate to 160-180 mg / L, and then add aspartic acid and heteroauxin; a subculture medium is to adjust concentration of boric acid to 2.0-2.2 mg / L on the basis of improved White culture medium, adjust concentration of molybdenum oxide to 0.0003-0.0005 mg / L, and further add vitamin K1, heteroauxin and naphthylacetic acid; a root medium is to separately add ammonium molybdate and gibberellin on the basis of the B5 culture medium, adjust pH value to 7.2 by whitewash. The culture medium is designed specific to growth characteristics of billbergia pyramidalis, and can meet the nutrition demands in every stage of the billbergia pyramidalis.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to an in vitro culture medium of water tamarind. Background technique [0002] Water Tower Flower (Billbergia pyramidalis Lindl.) is a perennial evergreen herbaceous succulent plant of Bromeliaceae, with very short stems. The leaves are broad-lanceolate, sharply pointed, with fine serrations on the edges, hard leathery, bright green, with thick cuticles and absorbing scales on the surface. The spikes are erect, higher than the leaves, the bracts are pink, the corolla is vermilion, the petals are curled, and the edges are purple. The leaves are convoluted and clustered from the rhizome, the base is rosette-shaped, and the center is cylindrical. Bloom more in winter and spring. The leaves are leathery; green and shiny, clustered into rosettes, dignified and beautiful; the bases of the leaves are embracing each other, making the center of the plant into a tube shape, which ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 不公告发明人
Owner 日照新睿招商发展有限公司