Method for extracting Bidens pilosa L. effective components and application of Bidens pilosa L. effective components to mask production

An extraction method and technology for active ingredients, which are applied in the field of extraction method for active ingredients of Pseudomonas spp The effect of improving the purity and improving the yield

Inactive Publication Date: 2017-03-08
杨攀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above 4 different extraction methods can obtain the effective components of P. balata for different purposes,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for extracting the effective components of the ghost needle grass comprises the following steps:

[0029] 1) First, remove the soil and weeds on the leaves of the devil's needle, wash the leaves of the devil's needle with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the devil's needle to reach 1000-2000 mesh, and obtain Ghost Needle Leaf Powder;

[0030] 2) Add 5 times the amount of water to soak the ghost needle grass leaf powder obtained in step 1) according to the weight of the ghost needle grass leaf powder. The pH is 6-7, the temperature is 40°C, the stirring speed is 35rpm, and the enzymolysis is 4hr; after the enzymatic hydrolysis of cellulase, add the compound protease with the weight of 1‰ of ghost needle grass leaves, adjust the pH to 6-7, and the temperature is 40 ℃, the stirring speed is 35rpm, enzymatic hydrolysis for 4hr; after enzymatic hydrolysis with the compound protease, treat at...

Embodiment 2

[0040] The method for extracting the effective components of the ghost needle grass comprises the following steps:

[0041] 1) First, remove the soil and weeds on the leaves of the devil's needle, wash the leaves of the devil's needle with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the devil's needle to reach 1000-2000 mesh, and obtain Ghost Needle Leaf Powder;

[0042] 2) Add 8 times the amount of water to soak the ghost needle grass leaf powder obtained in step 1) according to the weight of the ghost needle grass leaf powder. The pH is 6-7, the temperature is 45°C, the stirring speed is 30rpm, and the enzymolysis is 3hr; after the enzymatic hydrolysis of cellulase, add the compound protease with the weight of 3‰ of ghost needle grass leaves, adjust the pH to 6-7, and the temperature is 45 ℃, the stirring speed is 30rpm, enzymatic hydrolysis for 3hr; after enzymatic hydrolysis with compound protease, treat at 95℃...

Embodiment 3

[0052] The method for extracting the effective components of the ghost needle grass comprises the following steps:

[0053] 1) First, remove the soil and weeds on the leaves of the devil's needle, wash the leaves of the devil's needle with tap water, dry them in the shade, and then use an ultra-fine mechanical pulverizer to crush the leaves of the devil's needle to reach 1000-2000 mesh, and obtain Ghost Needle Leaf Powder;

[0054] 2) Add 10 times the amount of water to soak the ghost needle grass leaf powder obtained in step 1) according to the weight of the ghost needle grass leaf powder. The pH is 6-7, the temperature is 50°C, the stirring speed is 20rpm, and the enzymolysis is 2hr; after the enzymatic hydrolysis of the cellulase, add the compound protease with a weight of 5‰ of ghost needle grass leaves, adjust the pH to 6-7, and the temperature is 50 ℃, the stirring speed is 20rpm, enzymatic hydrolysis for 2hr; after enzymatic hydrolysis with the compound protease, treat...

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Abstract

The invention discloses a method for extracting Bidens pilosa L. effective components and application of the Bidens pilosa L. effective components to mask production. The method includes modern bioengineering techniques such as using cellulase to destroy cell walls so as to release flavonoid substances, using tannic acid to remove protein, using slightly-alkaline small-molecular-group water to extract the residual effective components of Bidens pilosa L. residues, and using a micro-filtration membrane to purify extract to obtain Bidens pilosa L. extract, namely the Bidens pilosa L. effective components. The method has the advantages that the extracted Bidens pilosa L. effective components are rich in antioxidant components such as polyphenols and flavonoids and antibacterial and anti-inflammation components and can be used for producing masks, and the method is high in extraction and separation yield.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from plants, in particular to a method for extracting active ingredients of P. punctatus and its application for preparing facial masks. Background technique [0002] Ghost needle grass, [Latin scientific name] Bidens pilosa L. [Alternative name] Guichai grass, shrimp claw grass, crab claw grass, pair of fork grass, sticky grass, sticky grass, bean dregs grass, annual herb with erect stems and blunt quadrangular shape. The lower leaves of the stem are smaller, rarely pinnate compound leaves with leaflets, and the leaflets on both sides are elliptic or ovate-elliptic. Flower heads 8-9 mm in diameter. Involucre base pubescent, strip-spatulate, upper part slightly wider. Tongue-shaped flowers, disc-shaped flowers, crown eaves 5-toothed. Achene black, strip-shaped, slightly flat, with ribs, with sparse tumor-like protrusions and setae on the upper part, 3-4 prickles on the top, with b...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/98A61K8/9794A61K8/67A61Q19/00A61Q19/02A61Q19/08A23K10/30
CPCA61K8/988A61K8/678A61K8/97A61K8/986A61K8/987A61Q19/00A61Q19/02A61Q19/08
Inventor 杨攀
Owner 杨攀
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