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Graphene oxide modified cetuximab as well as preparation method and application of graphene oxide modified cetuximab

A cetuximab and graphene technology, applied in the field of cetuximab, can solve the problems of weak efficacy of cetuximab, and achieve the effects of enhancing biological response, reducing irritation and stable properties

Inactive Publication Date: 2017-03-08
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a graphene oxide-modified cetuximab to solve the technical problem of weak curative effect of the existing cetuximab

Method used

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  • Graphene oxide modified cetuximab as well as preparation method and application of graphene oxide modified cetuximab
  • Graphene oxide modified cetuximab as well as preparation method and application of graphene oxide modified cetuximab
  • Graphene oxide modified cetuximab as well as preparation method and application of graphene oxide modified cetuximab

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preparation example Construction

[0037] Another aspect of the present invention provides a method for preparing graphene oxide-modified cetuximab, comprising the following steps: adding cetuximab and graphene oxide to a phosphate buffer solution with a volume percentage of 10-50% shake well at 4-40° C., and react for 2-24 hours to obtain the graphene oxide-modified cetuximab.

[0038] Phosphate buffer saline (PBS) is a commonly used reagent in this field, and its pH range is 7.35-7.45. The volume percentage is 10-50% phosphate buffer saline solution, that is, PBS solution, and the addition of water is adjusted on the basis of the original formula to dilute it.

[0039] Cetuximab and graphene oxide are reacted under the condition of shaking and shaking, and the two are combined by non-covalent bond to obtain graphene oxide-modified cetuximab. And the graphene oxide-modified cetuximab can be separated from the phosphate buffer as a solvent, or it can be kept in the phosphate buffer without separation.

[0040...

Embodiment 1

[0055] Graphene oxide raw materials were purchased from Sigma, purified by dialysis to remove alcohol, and prepared to a concentration of 1mg / ml. Under the condition of Misonix ultrasonic breaker Sonicator 3000 intensity 3.5 in the United States, the ultrasound was continued for 2 hours, the mode worked for 10 seconds, and rested for 5 seconds. Note that the whole process is carried out in a mixture of ice and water. After sonication, filter with a 0.22 μm filter to prepare graphene oxide, measure the final concentration with Nanodrop, and refrigerate for future use.

[0056] Add 1 mg of cetuximab and 0.2 mg of graphene oxide into 1 ml of 10% by volume phosphate buffer, shake well at 4°C, and react for 12 hours to obtain graphene oxide-modified cetuximab resistant mixture.

Embodiment 2

[0058] The difference from Example 1 is that the temperature for preparing graphene oxide-modified cetuximab is 37°C.

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Abstract

The invention discloses graphene oxide modified cetuximab as well as a preparation method and application of graphene oxide modified cetuximab. The graphene oxide modified cetuximab comprises cetuximab and graphene oxide, which are connected with each other by virtue of a non-covalent bond, the graphene oxide is two-dimensional nano graphene oxide, and the particle size of the graphene oxide is less than 0.22 micrometer. According to the graphene oxide modified cetuximab, the cetuximab and the graphene oxide are connected with each other by virtue of a non-covalent bond to form a compound antibody, so that a biological reaction can be greatly enhanced. Compared with cetuximab which only has a slight inhibitory action in vitro, the cetuximab can directly inhibit or kill and wound glioma cells. Meanwhile, by adopting the two-dimensional nano graphene oxide of which the particle size is less than 0.22 micrometer, the graphene oxide modified cetuximab is stable in property, and the stimulation to peripheral blood vessels is reduced at the same time.

Description

technical field [0001] The present invention relates to the field of antibodies, in particular to a graphene oxide-modified cetuximab. In addition, the present invention also relates to a preparation method and application of the graphene oxide-modified cetuximab. Background technique [0002] Targeted therapy refers to drugs or small molecule substances that act on specialized molecules to prevent tumor growth, proliferation, and spread. It is also called molecularly targeted therapy, molecularly targeted drugs, and precision medicine. Targeted therapy is different from chemotherapy: (1) Targeted therapy only acts on tumor-related molecules, while chemotherapy inhibits cell metabolism, affects cell cycle, and acts on all rapidly dividing cells, including tumors and normal cells. In other words, the specificity of chemotherapy is relatively poor; (2) Targeted therapy is carefully designed and selected to target cells or targets, while chemotherapy is selected only because i...

Claims

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Application Information

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IPC IPC(8): A61K47/52A61K39/395A61P35/00
CPCA61K39/395
Inventor 刘志雄罗承科杨魁李春涛曾瑜陈新建王锡阳黄正龚璇李臻琰
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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