Method used for preparing algae particle potassium fertilizer via compound microorganism fermentation
A technology of compound microorganisms and seaweed granules is applied in the field of compound microorganism fermentation to prepare seaweed granules potassium fertilizer, which can solve the problems of immature compounding process of bacteria and chemical fertilizers, rapid decline of microbial counts, rotten and rotten seedlings and rotten roots of microorganisms, and achieves environmental protection. The effect of improving performance and fertilizer efficiency, improving environmental adaptability, and extending shelf life
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Embodiment 1
[0035] 1) Preparation of product A: Wash and remove fresh kelp and sargassum (mass ratio: 1:1), soak for 20 hours, grind, transfer to a fermenter, sterilize at 110°C for 30 minutes, and cool to 20°C get product A;
[0036] 2) Prepare seed solution: activate and expand Bacillus cereus to prepare Bacillus cereus seed solution X; activate and expand Bacillus megaterium to prepare Bacillus megaterium seed solution Y; activate and expand Bacillus amyloliquefaciens Cultivate to prepare Bacillus amyloliquefaciens seed solution Z;
[0037] The medium formula of described Bacillus cereus activation and expansion cultivation is: soybean meal powder 20g, yeast extract 6g, sodium alginate 1.2g, bovine bile salt 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, Magnesium sulfate 0.2g, mannitol 9.5g, water 1000mL, adjust pH 6.8-7.0, expansion culture conditions: 33°C for 20h.
[0038] The medium formula for the activation and expansion of Bacillus megaterium is: sweet pot...
Embodiment 2
[0048] 1) Preparation of product A: Wash fresh copper algae, remove impurities, soak for 24 hours, refine, transfer to a fermenter, sterilize at 120°C for 15 minutes, and cool to 20°C to obtain product A;
[0049] 2) Preparation of seed liquid: the operation method is the same as in Example 1.
[0050] 3) Preparation of product B: Inoculate seed solution X into product A, the amount of seed solution X is 12% of the quality of product A, the fermentation temperature is 32°C, the pH value of the reaction is adjusted to 6.6 with an acid-base regulator, and the dissolved oxygen is controlled At 25%, the rotating speed is 180r / min, the ventilation rate is 850L / min, the pressure of the fermenter should be maintained at 0.03Mpa, and the fermentation time is 21h, to obtain product B;
[0051] 4) Preparation of product C: adjust the pH of product B to 4.5, cool the temperature in the fermenter to 4°C, store for 1 day, and then return the temperature to 25°C to obtain product C;
[005...
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