Super-low field nano magnetic probe, manufacturing method thereof and applications

An ultra-low field, magnetic probe technology, applied in scanning probe technology, scanning probe microscopy, measurement devices, etc., can solve problems such as laborious, time-consuming, and impact on applications, and achieve common equipment and simple components. , powerful effect

Active Publication Date: 2017-03-15
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ultra-low-field magnetic probes currently used are all micron-scale, and the components are magnetic nanoparticles and polymer assemblies. It is necessary to synthesize magnetic nanoparticles first, and then synthesize magnetic polymer microspheres, which is time-consuming and laborious.
At the same time, these ultra-low field magnetic probes will precipitate in the solution, which greatly affects their applications in ultra-low field magnetic detection, magnetic labeling, and magnetic imaging.

Method used

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  • Super-low field nano magnetic probe, manufacturing method thereof and applications
  • Super-low field nano magnetic probe, manufacturing method thereof and applications
  • Super-low field nano magnetic probe, manufacturing method thereof and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055]1. Add 2mmol of iron acetylacetonate, 6mmol of oleic acid, 6mmol of oleylamine and 20ml of octadecene into a 150ml three-necked flask, stir and mix evenly at 1600rpm / min, vacuumize at 80°C for 30min, then fill with nitrogen, and then heat up to 200°C ℃, keep it for 30min, then raise the temperature to 300℃, reflux for 30min, remove the heat source, cool to room temperature, then add an appropriate amount of ethanol, centrifuge at 6000r / min for 10min, take the precipitate, then add an appropriate amount of cyclohexane to dissolve the product, Centrifuge again at 6000r / min for 10min, take the upper liquid, repeat the ethanol precipitation / cyclohexane dispersion process 3 times, and finally use the prepared magnetic nanoparticles directly for the next experiment, or disperse them in cyclohexane and store them in a sample bottle and placed in a refrigerator at 4°C.

[0056] 2. Add 100mg of DHCA (3,4-dihydroxyhydrocinnamic acid) and 12ml of THF to a 100ml four-necked flask (1...

Embodiment 2

[0060] Add 2mmol of iron acetylacetonate, 6mmol of oleic acid, 6mmol of oleylamine and 20ml of octadecene into a 150ml three-necked flask, stir and mix evenly at 1600rpm / min, vacuumize at 80°C for 30min, then fill with nitrogen, and then heat up to 200°C. Keep it for 2h, then raise the temperature to 300°C, reflux for 1h, remove the heat source, cool to room temperature, then add an appropriate amount of ethanol, centrifuge at 6000r / min for 10min, take the precipitate, then add an appropriate amount of cyclohexane to dissolve the product, 6000r / min Min was centrifuged again for 10 min, the upper layer liquid was taken, and the process of ethanol precipitation / cyclohexane dispersion was repeated three times, and finally the prepared magnetic nanoparticles were directly used in the next experiment.

[0061] Subsequently, add 50mgDHCA (3,4-dihydroxyhydrocinnamic acid) and 6ml of THF to a 50ml four-necked flask (19*14×3), and connect the four ports of the four-necked flask to therm...

Embodiment 3

[0065] Add 2mmol of iron acetylacetonate, 6mmol of oleic acid, 6mmol of oleylamine and 20ml of octadecene into a 150ml three-necked flask, stir and mix evenly at 1600rpm / min, vacuumize at 80°C for 30min, then fill with nitrogen, and then heat up to 200°C. Keep it for 15 minutes, then raise the temperature to 300°C, reflux for 1 hour, remove the heat source, cool to room temperature, then add an appropriate amount of ethanol, centrifuge at 6000r / min for 10min, take the precipitate, then add an appropriate amount of cyclohexane to dissolve the product, 6000r / min Centrifuge again for 10 min, take the upper layer, repeat the ethanol precipitation / cyclohexane dispersion process 3 times, and finally disperse the prepared magnetic nanoparticles in cyclohexane and store them in a sample bottle and place them in a refrigerator at 4°C .

[0066] The dynamic light scattering (DLS) of the magnetic nanoparticle prepared by this embodiment measures the histogram of the hydrodynamic particle...

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Abstract

The invention belongs to the nano biological technology field and particularly relates to a super-low field nano magnetic probe, a manufacturing method thereof and applications. The super-low field nano magnetic probe is a magnetic nano particle modified by a surface hydrophilic ligand; the magnetic nano particle is a ferrite magnetic nano particle or a doped ferrite magnetic nano particle, and a doping agent of the doped ferrite magnetic nano particle is at least one of zinc, cobalt and manganese; the hydrophilic ligand is 3,4-dihydroxyphenylpropionic acid, 2,3-dimercaptosuccinic acid and PEG modified by double carboxyls; at least one application of the super-low field nano magnetic probe described as 1)-3) further belongs to a protection scope of the invention, 1), cell magnetic imaging; 2), bacteria detection; and 3), preparing magnetic immunization kits on the basis of optics atom magnetic instruments. The super-low field nano magnetic probe is advantaged in that the super-low field nano magnetic probe is simple and efficient, low cost is realized, re-assembling is not needed, mass-scale production can be carried out, good monodispersion, good biological compatibility and excellent magnetic property are realized, and the super-low field nano magnetic probe has multiple applications.

Description

technical field [0001] The invention belongs to the field of nano-biotechnology, and in particular relates to an ultra-low-field nano-magnetic probe and its preparation method and application. Background technique [0002] The ultra-low field optical atomic magnetometer can detect weak magnetic fields. The application of optical atomic magnetometer mainly includes the detection of ultra-low field remanence properties of materials and the use of ultra-low field magnetic probes to label cells, bacteria and biological macromolecules, and then has biological properties such as magnetic sensing, magnetic imaging and biological force spectroscopy. field applications. However, one of the keys to the application in the biological field lies in the preparation of ultra-low field magnetic probes. Ultra-low field magnetic probe is an important medium used in ultra-low field magnetic sensing, magnetic labeling, and magnetic imaging. It plays an important role in improving the sensitiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01Q60/54G01N33/543
CPCG01N33/54326G01N33/54346G01Q60/54
Inventor 姚立柴亚红王秀瑜
Owner INST OF CHEM CHINESE ACAD OF SCI
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