A molecular marker related to boar sperm motility traits and its application

A technique for sperm motility and boars, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems such as insufficient sperm motility, azoospermia, and sperm morphology defects
CN106544412BActive Publication Date: 2020-12-04HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2020-12-04

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Abstract

The invention belongs to the technical field of livestock molecular marker preparation, and concretely relates to a molecular marker related to a sperm activity character of a boar and application. The molecular marker is screened from a PRM2 gene; a nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. An allelic mutation R exists in nucleotide in position 169 of a sequence table, R is G or A, and the mutation causes the polymorphism of NaeI-RFLP. The invention further discloses a primer pair for amplifying PRM2 gene SNP (Single Nucleotide Polymorphism), and the primer pair is also the primer pair for detecting the screened molecular marker provided by the invention. The invention builds a method for detecting the polymorphisms of the sperm activity character of the boar. Association analytic application shows that the molecular marker provided by the invention can be used as the marker for assistant selection of the sperm activity character of the boar.
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Description

technical field

[0001] The invention belongs to the technical field of screening and application of pig molecular markers, and in particular relates to a molecular marker related to boar sperm motility traits and its application. The molecular marker screened in the present invention is cloned from the PRM2 gene. Background technique

[0002] The pig industry plays a pivotal role in my country's agriculture. In 2015, the number of pigs slaughtered nationwide reached 708.25 million heads, ranking first in the number of pigs slaughtered in the world during the same period. my country has become a veritable pig-raising country. Improving the fertility of boars is the key to pig production, and sperm motility is the main manifestation of the fertility of boars. Studies have shown that the sperm motility of breeding boars is extremely significantly correlated with the litter size and live piglets of Large White, Landrace and Duroc sows, and the sperm density is also related to ...

Claims

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