Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of high-activity haematococcus pluvialis cells

A technology of Haematococcus pluvialis and cells, which is applied in the fields of microalgae cultivation, seaweed deep processing and fermented food, and can solve the problems of low cell viability, weak tolerance of Haematococcus pluvialis cells, and weak anti-bacterial pollution ability , to achieve the effect of improving tolerance

Active Publication Date: 2017-04-19
QINGDAO KEHAI BIOLOGICAL +1
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Haematococcus pluvialis cell culture and production generally adopts photoautotrophic culture, but light has become the main limiting factor of microalgal cell growth in the process of photoautotrophic culture, often making the cell concentration very low during the culture process
[0010] 2. Generally, the cell viability of Haematococcus pluvialis cell culture is low, and it is easily affected by cell growth and metabolism, culture medium and environmental factors.
[0011] 3. Haematococcus pluvialis cells have weak tolerance to the culture environment and weak anti-bacterial pollution ability, which in turn affects cell growth status, cell viability and pure culture, and it is difficult to achieve high concentration of Haematococcus pluvialis cells nourish

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of high-activity haematococcus pluvialis cells
  • Preparation method of high-activity haematococcus pluvialis cells
  • Preparation method of high-activity haematococcus pluvialis cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This example is an industrial-scale Haematococcus pluvialis cell culture and preparation process carried out in a culture system containing a light culture fermenter.

[0051] In this embodiment, the strains of Haematococcus pluvialls are selected from strains of Haematococcus pluvialls strain 712, strains of Haematococcus pluvialls GB-235 and strains of Haematococcus pluvialls NIES-144. The strains and characteristics of Haematococcus pluvialis are shown in Table 4.

[0052] Concrete steps of the present invention include:

[0053] First, the breeding and domestication treatment of strains, the strains used: through strain selection and pure species isolation, the cells in the selected Haematococcus pluvialis strains are well developed, and the cells contained in them have fast growth rate, high cell viability, Pure cells with high astaxanthin biotransformation activity. The content of astaxanthin in Haematococcus pluvialis algal powder is more than 3%. The cells ar...

Embodiment 2

[0072] In this example, the cell culture process of Haematococcus pluvialis was carried out in a laboratory 10L culture system containing a culture fermenter with light, and the small-scale cell preparation process of Haematococcus pluvialls was carried out by simulating the cell culture process of factory production.

[0073] Under laboratory conditions, the process and process of Haematococcus pluvialis cell culture in the factory were simulated, and it was carried out in a 10L culture system. The test material of this example is Haematococcus pluvialis GB-235 bacterial strain. The morphology, physiological characteristics and strain characteristics of Haematococcus pluvialis strains are shown in Table 7.

[0074] Table 7 Haematococcus pluvialis strains and their characteristics

[0075]

[0076] Haematococcus pluvialis cell culture refers to the factory production cell culture process conditions, the specific parameters are as follows:

[0077] (1) Expanded culture of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of high-activity haematococcus pluvialis cells, and belongs to the technical field of microalgae culture, seaweed deep processing and fermentation food. According to the preparation method, CO2, an organic carbon source and nutrition salts are used as a culture medium; haematococcus pluvialis is used as a microalgae culture strain; a self culture state and mixed state culture method is used; and the method comprises the steps and processes of strain breeding and domestication treatment, haematococcus pluvialis cell amplification culture, fermentation tank culture and culture. The high-activity haematococcus pluvialis cells produced by the preparation method provided by the invention has the advantages that the haematococcus pluvialis cells have high generated cell concentration, high cell activity and high astaxanthin biological transformation activity; and haematococcus pluvialis powder with high astaxanthin content can be prepared.

Description

technical field [0001] The invention belongs to the technical fields of microalgae cultivation, seaweed deep processing and fermented food. The invention relates to a preparation method suitable for cells containing high vigor of Haematococcus pluvialls, in particular to a method suitable for high-efficiency cultivation of Haematococcus pluvialls cells and its preparation, endowing the haematococcus pluvialls cells with high vigor cells and astaxanthin Biotransformation activity and high cell concentration can prepare high-viability Haematococcus pluvialis cells. Background technique [0002] Haematococcus pluvialis, also known as Haematococcus pluvialis. It is a kind of unicellular green alga widely distributed in nature, and belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococcus, and Haematococcus in taxonomy. The cells are oval or elliptical. The size of swimming cells is 3-5 μm in width and 3-8 μm in length; the size of immobile cells is 19-51 μm in width and 28-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12N1/36C12R1/89
CPCC12N1/12C12N1/36
Inventor 李悦明管斌李霞徐建春孔青韩建友曲文强
Owner QINGDAO KEHAI BIOLOGICAL