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Lysinibacillus macroides capable of enriching phosphorus and degrading organophosphorus pesticides and application of lysinibacillus macroides

An organophosphorus pesticide and phosphorus-rich technology, which is applied in the field of microorganisms to achieve good application prospects, reduce eutrophication, and avoid inorganic phosphorus pollution.

Active Publication Date: 2017-04-19
山东省神农生态科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, domestic and foreign researchers have reported some Lysinibacillus The research on the biological effects of some strains mainly focuses on the degradation of dyes in wastewater, such as patent CN 103937697 A; the production of extracellular protease and intracellular esterase such as ethyl carbamate hydrolase, such as patent CN 103013948 A; used in the environment Reduction of hexavalent chromium ions, such as patent CN103395893 A; degradation of negative divalent sulfide ions in petrochemical wastewater, such as patent CN 102827786 A; dissolution or infection of cyanobacteria, such as patent CN 102965298 A; degradation of toxic substances polybrominated diphenyl ethers, such as patent CN 102363756 A; and about Lysinibacillus However, there are few reports on the degradation of organophosphorus pesticides, and only a small amount of literature involves

Method used

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  • Lysinibacillus macroides capable of enriching phosphorus and degrading organophosphorus pesticides and application of lysinibacillus macroides
  • Lysinibacillus macroides capable of enriching phosphorus and degrading organophosphorus pesticides and application of lysinibacillus macroides
  • Lysinibacillus macroides capable of enriching phosphorus and degrading organophosphorus pesticides and application of lysinibacillus macroides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Lysinibacillus macroides Isolation and identification of MEW88

[0021]Collect soil and water samples near a phosphate chemical factory in Yichang, weigh 2g of soil and water samples into 100mL LB culture medium, and enrich and culture at 28°C and 150 r / min for 3 days. The formula of LB medium is as follows: Peptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, water 1L, pH7.0-7.2. Inoculate the above bacterial suspension into the fermentation medium containing glyphosate and dimethoate (peptone 2 g / L, yeast extract 1 g / L, NaCl 2 g / L, Water 1L, pH7.0-7.2), 28°C, 150 r / min enrichment culture for 7 days, transfer to the same medium, continue to cultivate and acclimatize under the same conditions, gradually increase glyphosate and le fruit content until its concentration was 800 mg / L; the acclimatized and stable bacterial liquid was streaked on the inorganic salt medium (glucose10.0 g, (NH 4 ) 2 SO 4 0.5 g, NaCl 0.3 g, KCl 0.3 g, MgSO 4 •7H 2 O 0.3g, FeSO 4 •...

Embodiment 2

[0024] Example 2 Lysinibacillus macroides MEW88 phosphorus enrichment test

[0025] Take Example 1 Lysinibacillus macroides MEW88 at 28 o Activate overnight at C 150 r / min, centrifuge the activated bacterial solution, remove the supernatant, wash the pellet once with sterilized PBS (pH 7.2), resuspend and adjust the concentration of the bacterial solution to OD 600 =1.0. Inoculate the above-mentioned bacterial suspension into 200mL PAM medium with a phosphorus content (calculated as P) of 10 mg / L and 20 mg / L at an inoculation amount of 10% (V / V), at 28°C and 150rpm Shake flask culture, sampling at 0, 12, 24, 48, 72 hours, after centrifugation at 8000r / min for 10min, measure the supernatant inorganic phosphorus content (GB 11893-89-molybdenum antimony anti-colorimetric method). The PAM medium component is: Na 3 C 6 h 5 o 7 4.0 g / L, NaCl0.5 g / L, (NH4) 2 SO 4 2.5 g / L, CaCl 2 0.25 g / L, MgSO 4 0.25 g / L, KH 2 PO 4 0.0878 g / L (phosphorus content 20mg / L), maltose 0.01 g / ...

Embodiment 3

[0029] Example 3 Lysinibacillus macroides Test of MEW88 on degradation effect of different concentrations of dimethoate

[0030] Take Example 1 Lysinibacillus macroides After overnight activation of MEW88, wash the bacteria once with PBS (pH 7.2-7.4), resuspend and adjust the OD of the bacteria solution 600 It was 2.0, and then transferred to the fermentation medium containing different concentrations of dimethoate (50mg / L, 100 mg / L, 150 mg / L, 200 mg / L,) according to the inoculation amount of 4% (V / V). Periodic sampling was carried out to determine the degradation rate of organophosphorus pesticides, and the results were as follows: image 3 .

[0031] From image 3 It can be seen that MEW88 has a high ability to degrade dimethoate, especially at a low concentration of 50 mg / L, the degradation rate of the strain reached 97.5% on the 5th day, and basically no dimethoate was detected on the 7th day. Therefore, this bacterium has good application value in aspects such as dim...

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PUM

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Abstract

The invention discloses a lysinibacillus macroides capable of enriching phosphorus and degrading organophosphorus pesticides. The strain is lysinibacillus macroides MEW88, which is preserved in China Center for Type Culture Collection on October 18, 2016 with preservation number of CCTCC NO:M2016574. The strain lysinibacillus macroides has an instant and efficient phosphorus enriching capacity and is capable of effectively degrading dimethoate and glyphosate; and the strain has a good application prospect in the aspect of biological treatment of phosphorus chemical industry wastewater.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of the genus Lysinobacillus that is rich in phosphorus and degrades organophosphorus pesticides Lysinibacillus macroides and applications. Background technique [0002] With the development and application of multiphase taxonomy, the genus Bacillus ( Bacillus sp. ) classification has changed a lot, and many species have been isolated from Bacillus and assigned to other genera or new genera have been established. Lysinibacillus ( Lysinibacillus sp. ) was recently obtained from the genus Bacillus ( Bacillus sp. ) is a new genus isolated from the genus Bacillus ( Bacillus sp. ) to distinguish. Bacillus lysinica ( Lysinibacillus sp. ) classification status is: Bacteria ; Firmicutes ; Bacilli ; Bacillales ; Bacillaceae ; Lysinibacillus . The strains of this genus are isolated from various environments such as soil, river bed sediment, pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/04A62D101/28C02F101/10C02F101/38
CPCA62D3/02A62D2101/04A62D2101/28C02F3/34C02F2101/105C02F2101/306C02F2101/38C02F2101/40C12N1/205C12R2001/01
Inventor 倪红刘诚万娟唐梦君邵闯
Owner 山东省神农生态科技股份有限公司
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