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Serum amyloid protein A detection method and reagent

A technology of serum starch and detection reagents, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsatisfactory automatic rapid batch detection, narrow linear range, high detection cost, etc., to achieve fast and simple detection and ensure accuracy , the effect of high accuracy

Inactive Publication Date: 2017-04-19
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Latex-enhanced rate scattering turbidimetry can only be used with a specific protein analyzer and the detection cost is relatively high. The immunocolloidal gold method can only be used with a gold standard digital quantitative analyzer, which cannot meet the requirements of automated rapid batch detection. The latex-enhanced immune turbidimetric method published in related patents in the past two years uses polyclonal antibody latex-enhanced method, which is suitable for automatic biochemical analyzers, but its linear range is narrow, and it needs to be continuously diluted if encountering high-concentration SAA samples in clinical applications. , cumbersome operation

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  • Serum amyloid protein A detection method and reagent
  • Serum amyloid protein A detection method and reagent
  • Serum amyloid protein A detection method and reagent

Examples

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Effect test

Embodiment 1

[0040] Serum amyloid A detection reagents include reagent R1 and reagent R2. Specifically:

[0041] Reagent R1 includes: buffer, surfactant, reaction accelerator, electrolyte, preservative, stabilizer. Wherein: the buffer solution is a Tris buffer solution with a pH value of 6.5 and a concentration of 100mmol / L, the surfactant is 0.01% Tween20 by mass percentage, the reaction accelerator is 5.00% polyethylene glycol 2000 by mass percentage, and the electrolyte is The mass percentage is 1.20% of sodium chloride, the preservative is 0.20% by mass of sodium azide, and the stabilizer is 0.90% by mass of bovine serum albumin.

[0042]Reagent R2 includes: latex cross-linked product, surfactant, stabilizer, preservative, electrolyte, buffer. Among them: the mixing ratio of large latex particles and small latex particles in the latex cross-linked product is 1:2, the percentage of large latex particles is 0.06%, coated with SAA rabbit anti-human polyclonal antibody, the particle size...

Embodiment 2

[0047] Serum amyloid A detection reagents include reagent R1 and reagent R2. Specifically:

[0048] Reagent R1 includes: buffer, surfactant, reaction accelerator, electrolyte, preservative, stabilizer. Wherein: the buffer solution is a glycine buffer solution with a pH value of 8.5 and a concentration of 100mmol / L, the surfactant is 2.00% Tween80 by mass percentage, the reaction accelerator is 0.01% polyethylene glycol 4000 by mass percentage, and the electrolyte is The mass percentage is 0.01% of magnesium chloride, the preservative is 0.01% by mass of lithium azide, and the stabilizer is 0.01% by mass of gelatin.

[0049] Reagent R2 includes: latex cross-linked product, surfactant, stabilizer, preservative, electrolyte, buffer. Among them: the mixing ratio of large latex particles and small latex particles in the latex cross-linked product is 1:16, the percentage of large latex particles is 0.01%, coated with SAA rabbit anti-human polyclonal antibody, the particle size is ...

Embodiment 3

[0054] Serum amyloid A detection reagents include reagent R1 and reagent R2. Specifically:

[0055] Reagent R1 includes: buffer, surfactant, reaction accelerator, electrolyte, preservative, stabilizer. Wherein: buffering solution is the HEPES damping fluid that pH value is 7.0, concentration is 50mmol / L, surfactant is the Emulgen B-66 that mass percent is 0.50%, and reaction accelerator is the polyethylene glycol 8000 that mass percent is 2.50% , the electrolyte is 1.50% potassium chloride by mass percentage, the preservative is 1.00% benzoic acid and salts by mass percentage, and the stabilizing agent is a mixture of gelatin (2.00%) and bovine serum albumin (2.00%) that is 4.00% by mass .

[0056] Reagent R2 includes: latex cross-linked product, surfactant, stabilizer, preservative, electrolyte, buffer. Among them: the mixing ratio of large latex particles and small latex particles in the latex cross-linked product is 1:8, the percentage of large latex particles is 0.03%, ...

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Abstract

The invention discloses a serum amyloid protein A detection method. The serum amyloid protein A detection method comprises following steps: serum SAA antigen of a tested body is mixed with a latex cross-linked substance coated with SAA antibody, in vitro agglutination reaction is carried out, and quantitative determination of the content of SAA in serum is carried out. The invention also discloses a serum amyloid protein A detection reagent. The serum amyloid protein A detection method and the reagent possesses following advantages: linearity range is wide, detection is rapid, detection sensitivity is high, accuracy is high, cost is low, and the reagent is stable.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a method for detecting serum amyloid A. The invention also relates to a reagent for detecting serum amyloid A. Background technique [0002] Serum amyloid A protein (SAA) is a polymorphic protein family encoded by multiple genes, the precursor of tissue amyloid A protein, and belongs to the acute phase response protein. During the period of inflammation or disease infection, SAA rises rapidly within 48-72 hours, which can increase to 1000 times of the initial concentration, and decreases rapidly during the recovery period of the disease (Urieli Shoval S, et, al. Expression and function of serum amyloid A, a major acute phase protein, in normal and disease states [J]. Curr Opin Hematol, 2000, 7(1):64-69.). SAA is elevated in both viral and bacterial infections, while CRP is hardly or not significantly elevated in viral infections. The combined detection of the two is ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803G01N2333/4737
Inventor 王剑林清菁
Owner PUREBIO LAB NINGBO
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