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Synthetic stent for promoting growth of bone cells and making method thereof

A technology of bone cells and composite materials, which is applied in the field of synthetic scaffolds promoting bone cell growth and its preparation, can solve the problems of slow blood vessel growth, general prognosis, and slow cell growth speed, and achieve high response repeatability, easy post-processing, The effect of the simple preparation process

Inactive Publication Date: 2017-04-26
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Osteogenic effects of existing scaffolds are greatly limited due to lack of vascular network formation
The role of the stent is not obvious, no blood vessels grow or the blood vessels grow slowly, resulting in slow cell growth, and the prognosis is average

Method used

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  • Synthetic stent for promoting growth of bone cells and making method thereof
  • Synthetic stent for promoting growth of bone cells and making method thereof
  • Synthetic stent for promoting growth of bone cells and making method thereof

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preparation example Construction

[0044] A kind of preparation method of the synthetic support that promotes bone cell growth of the present invention, comprises the following steps:

[0045]First, add 2g of chitosan into 100ml of acetic acid with a volume ratio of 2%, and stir to prepare chitosan acetic acid colloid; Look, it may actually be more than 72mg) Salvianolic acid B was dissolved in 1mL ethanol and added to the acetic acid of chitosan and stirred evenly, then the Ca(NO 3 ) 2 、KH 2 PO 4 Add to the mixed solution and stir for a long time to prepare salvianolic acid B-chitosan / hydroxyapatite precursor solution, wherein the molar ratio of Ca / P is 5:3, and the final mass of chitosan / hydroxyapatite The ratio is about 1:2;

[0046] Then, the precursor solution was stirred for 4-5 hours and then centrifuged to remove air bubbles; then the solution was poured into a plastic mold, placed in an environment of 4°C for 2 hours, and in an environment of -10°C for 3 hours to freeze the solution into a solid (o...

Embodiment 1

[0049] Salvianolic acid B (Sal B) (molecular formula: C 36 h 30 o 16 ,, molecular weight: 718.6) was purchased from sigma company (Tianjin, China); chitosan (CS) (molecular weight = 2.5 × 105, deacetylation degree ≥ 90%, viscosity > 100cps, biomedical grade) was purchased from Pioneer Biotechnology Company (Xi'an, China). First, 2g of chitosan was added to 100ml of 2% (V / V) acetic acid, and stirred for 20min to prepare a CS solution. Dissolve 72 mg of salvianolic acid B in 1 mL of ethanol and add it to the CS solution and stir for 2 h, then Ca(NO 3 ) 2 、KH 2 PO 4 (molar ratio Ca / P=1.67) was added to the mixed solution to prepare a Sal B-CS / HA precursor solution (the final mass ratio of CS / HA was 1 / 2). Then, the precursor solution was homogenized for 4 h and centrifuged for 10 min to remove air bubbles. The solution was poured into a plastic mold (diameter 4.0mm, length 20mm), placed at 4°C for 2h, -10°C for 3h, and then the solution was freeze-dried for 24h. After ful...

Embodiment 2

[0051] Salvianolic acid B (Sal B) (molecular formula: C 36 h 30 o 16 ,, molecular weight: 718.6) was purchased from sigma (Tianjin, China); chitosan (CS) (molecular weight = 2.5 × 105, degree of deacetylation ≥ 90%, viscosity > 100 cps, biomedical grade) was purchased from Pioneer Biotechnology Company ( Xi'an, China). First, 2g of chitosan was added to 100ml of 2% (V / V) acetic acid, and stirred for 20min to prepare a CS solution. Dissolve 48 mg of salvianolic acid B in 1 mL of ethanol and add it to the CS solution and stir for 2 h, then Ca(NO 3 ) 2 、KH 2 PO 4 (molar ratio Ca / P=1.67) was added to the mixed solution to prepare a Sal B-CS / HA precursor solution (the final mass ratio of CS / HA was 1 / 2). Then, the precursor solution was homogenized for 5 h and centrifuged for 10 min to remove air bubbles. The solution was poured into a plastic mold (diameter 4.0mm, length 20mm), placed at 4°C for 2h, -10°C for 3h, and then the solution was freeze-dried for 24h. After being ...

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Abstract

The invention discloses a synthetic stent for promoting the growth of bone cells and a making method thereof. The method includes the steps that first, chitosan is added into acetic acid, and the mixture is stirred to be completely dissolved to prepare an acetic acid colloid of the chitosan; then, salvia miltiorrhiza phenolic acid B is dissolved in ethyl alcohol, the mixture is added into the acetic acid of the chitosan to be stirred uniformly, so that a mixed solution is obtained, and Ca(NO3)2 and KH2PO4 are added into the mixed solution to prepare a salvia miltiorrhiza phenolic acid B-chitosan / hydroxylapatite precursor solution; then, the precursor solution is subjected to homogenation treatment, and then foams are removed through stirring and centrifuging; then the precursor solution is poured into a mold, the mold is put in the environment of 4 DEG C for 2 hours and the environment of 10 DEG C for 3 hours in sequence, and then the solution is subjected to freeze-drying for 24 hours; after sufficient drying, a salvia miltiorrhiza phenolic acid B-chitosan / hydroxylapatite stent is soaked in an NaOH solution for 2 hours and washed several times with distilled water; then, the stent is subjected to freeze-drying again, the two ends are cut off, and finally the synthetic stent for promoting the growth of the bone cells is obtained. The stent can stably and continuously release medicine within 56 days.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of bone scaffolds, in particular to a synthetic scaffold for promoting the growth of bone cells and a preparation method thereof. [0003] 【Background technique】 [0004] At present, there are scaffolds used to promote the growth of bone cells after bone injury, and the main material is hydroxyapatite (Shuai et al.2013). In order to overcome the brittleness of this material, adding chitosan to the subsequent scaffold can effectively improve the biocompatibility and biodegradability of the scaffold (Ma et al.2014). [0005] Osteogenesis-promoting effects of existing scaffolds are greatly limited due to the lack of vascular network formation (Phelps et al. 2010). [0006] Salvianolic acid B, extracted from Salvia miltiorrhiza, is one of the most effective pro-angiogenic substances (Guo et al.2014). For example, the following documents record: [0007] Shuai C, Gao C, Feng P, Peng S and Wen X (2013) ...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/46
CPCA61L27/46A61L27/54A61L2300/216A61L2300/412A61L2300/602A61L2430/02C08L5/08
Inventor 樊俊俊戴春秋吴岩林楷丰李东林李晓祥毕龙裴国献
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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