Clarithromycin hapten, artificial antigen and antibody and their preparation method and application

A technology of clarithromycin and hapten, which is applied in clarithromycin hapten, artificial antigen and antibody and its preparation method, can solve the problems of high cost, inapplicability to large-scale sample screening and detection, cumbersome operation steps, etc., and achieve Low cost, high sensitivity, and the effect of analyzing large sample volumes

Active Publication Date: 2019-05-10
深圳市绿诗源生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have strong specificity and high sensitivity, the sample pretreatment steps are cumbersome and costly, and they are not suitable for the screening and detection of large batches of samples.

Method used

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  • Clarithromycin hapten, artificial antigen and antibody and their preparation method and application
  • Clarithromycin hapten, artificial antigen and antibody and their preparation method and application
  • Clarithromycin hapten, artificial antigen and antibody and their preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of the clarithromycin hapten provided by the present invention is as follows: add 748.3 mg (1 mmol) of clarithromycin into a 50 ml reaction bottle, and add 10 ml of anhydrous acetonitrile to dissolve it. Weigh 120.4mg (1.2mmol) of succinic anhydride into the reaction flask, and measure 1ml of triethylamine into the reaction flask. Room temperature, magnetic stirring, reaction for 4 hours. Afterwards, spin evaporate under reduced pressure, concentrate, add 50ml of water, adjust the pH to about 5 with glacial acetic acid, precipitate an off-white solid, filter, take the filter cake, and crystallize with 15ml of a mixed solvent (V ethyl acetate: V n-hexane = 1:2) , filtered, and dried to obtain 237.6 mg of product, the structural formula of which is formula (I). Example 2

Embodiment 2

[0043] A preparation method of the clarithromycin hapten provided by the present invention is as follows: add 748.3 mg (1 mmol) of clarithromycin into a 50 ml reaction bottle, and add 10 ml of anhydrous acetonitrile to dissolve it. Weigh 100.2 mg (1 mmol) of succinic anhydride into the reaction flask, and measure 1 ml of triethylamine into the reaction flask. Room temperature, magnetic stirring, reaction for 3 hours. Afterwards, spin evaporate under reduced pressure, concentrate, add 50ml of water, adjust the pH to about 5 with glacial acetic acid, precipitate an off-white solid, filter, take the filter cake, and crystallize with 15ml of a mixed solvent (V ethyl acetate: V n-hexane = 1:2) , filtered, and dried to obtain 216.2 mg of product, the structural formula of which was formula (I).

Embodiment 3

[0045] A preparation method of the clarithromycin hapten provided by the present invention is as follows: add 748.3 mg (1 mmol) of clarithromycin into a 50 ml reaction bottle, and add 10 ml of anhydrous acetonitrile to dissolve it. Weigh 150.7mg (1.5mmol) of succinic anhydride into the reaction flask, and measure 1ml of triethylamine into the reaction flask. Room temperature, magnetic stirring, reaction for 5 hours. Afterwards, spin evaporate under reduced pressure, concentrate, add 50ml of water, adjust the pH to about 5 with glacial acetic acid, precipitate an off-white solid, filter, take the filter cake, and crystallize with 15ml of a mixed solvent (V ethyl acetate: V n-hexane = 1:2) , filtered, and dried to obtain 241.5 mg of product, the structural formula of which is formula (I).

[0046]

[0047]

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Abstract

The invention discloses a clarithromycin hapten, an artificial antigen and an antibody, and a preparation method and application thereof. The invention aims to provide the clarithromycin hapten which can maintain the feature structure of clarithromycin to the greatest degree and has an active group capable of coupling with carrier protein; the artificial antigen and antibody are prepared from the clarithromycin hapten to be used for detecting macrolide compounds; according to the technical scheme, the structure of the clarithromycin hapten is as shown in the following formula; the clarithromycin artificial antigen is prepared by coupling the clarithromycin hapten with the carrier protein; the invention belongs to the field of a biotechnology; and (the formula is as shown in the description).

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a clarithromycin hapten, an artificial antigen, an antibody and a preparation method thereof. Background technique [0002] Clarithromycin belongs to macrolide antibiotics and is effective against most Gram-positive bacteria, some Gram-negative bacteria and some atypical pathogenic bacteria (mycoplasma, chlamydia, etc.). It is one of the second-generation derivatives of erythromycin. The main members of the second-generation derivatives of erythromycin include roxithromycin, azithromycin, clarithromycin, and dirithromycin. Because its second-generation derivatives avoid being destroyed by gastric acid, improve the absorption rate, and have stronger antibacterial activity, they are widely used as first-line drugs for the treatment of respiratory tract infections. [0003] However, it has been reported recently that long-term and continuous administration of the second-ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08C07H1/00C07K14/765C07K14/795C07K16/44G01N33/53G01N33/577
CPCC07H1/00C07H17/08C07K14/765C07K14/795C07K16/44C07K19/00G01N33/53G01N33/577
Inventor 宁波李隆军刘明如赖启隆朱永利蒋永青吴育春
Owner 深圳市绿诗源生物技术有限公司
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