Oceanobacillus sp. XC22919 and application thereof

A technology of XC22919 and bacillus, which is applied to the application field of Pacific bacillus and its secondary metabolites in the field of quorum sensing inhibition, can solve problems such as difficulty in eradicating Pseudomonas aeruginosa, and achieve the effect of simple and easy-to-control preparation process and simple conditions.

Inactive Publication Date: 2017-04-26
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The eradication of Pseudomonas aeruginosa is particularly difficult due to strong resistance to traditional antibiotics

Method used

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  • Oceanobacillus sp. XC22919 and application thereof
  • Oceanobacillus sp. XC22919 and application thereof
  • Oceanobacillus sp. XC22919 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Isolation, purification and identification of marine bacteria

[0040] 1. Strain screening

[0041] The 10 sea mud samples were collected from different depths in the Pacific seamount, and the 10 samples were placed in sterile sample bags and stored at -20°C. The 10 sea mud samples were subjected to the following operations to screen bacteria.

[0042] (1) Take 5g of spare sea mud, add an appropriate amount of sterile seawater in a sterile mortar, grind it into fine particles, place it in a conical flask pre-filled with sterilized LB liquid medium, and culture it with shaking at 30°C and 180rpm , enriched for 12 hours to obtain enriched sea mud suspension.

[0043] (2) Then take 1ml of the enriched sea mud suspension and carry out gradient dilution with sterile deionized water to obtain 10 -1 -10 -9 Nine dilutions of sea mud suspension.

[0044] (3) Take 100 μL of the sea mud suspension of each dilution and spread it on the LB plate, culture it in a cons...

Embodiment 2

[0072] Example 2: Growth Inhibition and Violet Inhibition of Violet Bacillus ATCC12472 by Pacibacterium XC22919

[0073] 1. The effect of different concentrations of Pacific bacillus XC22919 extract on the purple pigment production of C.violaceum ATCC12472

[0074] 1.1 C.violaceum ATCC12472 bacteria solution and culture medium preparation

[0075] 1.1.1 Pick a single colony of Violet Bacillus ATCC12472 from the plate and inoculate it in a pre-prepared conical flask (250ml conical flask, 100ml medium) filled with LB liquid medium, cultivate overnight at 30°C, 180rpm to logarithm During the period, the violaceum ATCC12472 bacterial liquid was obtained for future use.

[0076] 1.1.2 Use 27 conical vials of 25ml, and divide them into 3 groups in total, which are blank group, negative control group and experimental group, among which 7 concentration gradients are set in the experimental group, and each group has 3 parallel samples. Put 10ml LB liquid culture medium in the vial, ste...

Embodiment 3

[0091] Example 3: Pyocyanin inhibition and cluster motility inhibition of wild-type Pseudomonas aeruginosa by Pacibacterium XC22919

[0092] 1. Pyocyanin inhibition of wild-type Pseudomonas aeruginosa by Pacific bacillus XC22919

[0093] 1.1.1 Inoculate wild-type Pseudomonas aeruginosa in PB liquid medium, grow to the logarithmic phase at 37°C, and divide 27 25ml Erlenmeyer vials into 3 groups, namely blank group and negative control group , the experimental group, wherein the experimental group is set with 7 concentration gradients, and each group has 3 parallel samples, and 10ml of PB liquid medium is put in each conical vial, sterilized by high temperature and high pressure, and set aside.

[0094] 1.1.2 Dilute Pseudomonas aeruginosa bacteria solution with fresh PB liquid medium, adjust the concentration of the bacteria solution to OD≈0.05, and add it to the conical vials of each experimental group and negative control group (except the blank group). 10ml of bacterial solu...

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Abstract

The invention discloses Oceanobacillus sp. XC22919 and application in preparation of a quorum sensing bacteriostatic agent. Fermentation culture conditions of the Oceanobacillus sp. XC22919 are simple, the preparation technological process of active secondary metabolites is simple and easy to control, prepared metabolites have the advantages of obviously inhibiting bacterial quorum sensing activity and the like, and the optimal effective concentration of quorum sensing inhibition, namely, the quorum sensing inhibition activity maximum concentration within the minimum inhibitory concentration range of chromobacterium violaceum is 0.15 mg/ml.

Description

[0001] (1) Technical field [0002] The invention relates to the field of marine microorganisms and antibiotics, specifically the application of a strain of Pacific bacillus (Oceanobacillus sp. XC22919) and its secondary metabolites in quorum sensing inhibition. [0003] (2) Background technology [0004] Bacterial infection is an acute systemic infection caused by pathogenic bacteria or conditional pathogenic bacteria invading the blood circulation, growing and multiplying, and producing toxins and other metabolites. Bacterial infections have always threatened human health, and humans are looking for and fighting against these seemingly inconspicuous microorganisms. Penicillin began to be used clinically and successfully solved the problem of Staphylococcus aureus infection, creating a new era in the treatment of bacterial infectious diseases. Subsequently, a variety of antibiotics were discovered at an alarming rate. After half a century of rapid development, most of the acu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P31/04C12R1/01
CPCA61K35/74C12N1/205C12R2001/01
Inventor 王鸿陈小春严银春张甲生许学伟
Owner ZHEJIANG UNIV OF TECH
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